大鼠干扰素-γ ELISA定量试剂盒(IFN-γ)

大鼠干扰素‑γ ELISA定量试剂盒 (Rat IFN‑γ ELISA Kit) - KTE9017‑1

产品概述

本套件为细胞分析核心产品，专为大鼠来源的干扰素‑γ (IFN‑γ) 定量检测设计，采用高灵敏度夹心ELISA技术，适用于细胞培养上清、血清、血浆及其他生物液体的研究工作。

主要应用

• 蛋白类研究
• ELISA应用（夹心法）
• 免疫调节、抗病毒、抗菌及免疫应答机制的功能验证

技术参数

检测指标	IFN‑γ（大鼠）
检测范围	150 pg/mL – 2400 pg/mL
检测灵敏度	15 pg/mL
样本类型	细胞培养上清、血清、血浆、其它生物液体
检测方法	夹心ELISA（双抗体捕获‑检测体系）

试剂盒组成

• 大鼠源 IFN‑γ 抗体预包被微孔板
• 大鼠源 IFN‑γ 蛋白标准品
• 大鼠源 IFN‑γ 检测抗体
• EliKine™ Streptavidin‑HRP
• 标准品稀释液
• 实验缓冲液
• HRP底物
• 反应终止液
• 洗涤缓冲液
• 封板膜

特点 & 优势

• 高灵敏度：最低检测限 15 pg/mL，满足低丰度样品的定量需求。
• 特异性强：对大鼠 IFN‑γ 具备优异的选择性，未观察到显著交叉反应或背景干扰。
• 采用 EliKine™ Streptavidin‑HRP 体系，信号放大一致可靠。
• 全套即用配方，简化实验流程，适合高通量筛选。

操作要点（推荐）

• 试剂需在使用前于室温（≈20‑25 °C）平衡 30 分钟。
• 全过程使用一次性吸头，防止交叉污染。
• 未使用的板条请重新密封并置于 4 °C 保存。
• 混匀关键：建议使用低速振荡器或每 10 分钟手动轻摇一次。
• 样品与标准建议设置双孔或三孔（复孔）以提高数据可靠性。

保存与运输

• 未开封试剂盒：保存于 2‑8 °C 冰箱。
• 开启后请依据说明书对各组分进行分装保存。
• 运输方式：冰袋（蓝冰）冷链运输，确保全程温度控制。

规格 & 价格

规格	每套含孔数	单价 (人民币)	交货周期
48 T	48 孔	1,598.00	期货（次日发货）
96 T	96 孔	2,558.00	期货（次日发货）

警示与合规

仅限科研用途，本产品不适用于人类或临床诊断。使用本试剂盒进行的任何专利或许可证相关行为，均由使用者自行承担风险，供应商不承担相应责任。

背景知识

干扰素 (IFNs) 是宿主细胞在病毒、细菌、寄生虫或肿瘤等病原体侵袭时产生的关键免疫信号分子。II 型干扰素 IFN‑γ 以二聚体形式分泌，主要来源于 NK、NKT、CD4⁺ Th1 及 CD8⁺ 细胞毒性 T 细胞，在先天免疫和适应性免疫中均发挥关键作用。IFN‑γ 能激活巨噬细胞、促进 MHC II 表达，且与多种自身炎症及自身免疫疾病的异常表达密切相关，因而成为免疫学研究中常用的功能标记。

参考信息

• 基因 ID：25712
• 蛋白质 ID：P01581 (UniProt)
• 常用别名：IFG、IFI、IFN‑γ、Interferon gamma、Macrophage Activating Factor 等

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Rat IFN‑γ ELISA Quantitation Kit - KTE9017‑1

Product Overview

This kit is a core product for cell analysis, specifically designed for the quantitative detection of rat interferon-gamma (IFN‑γ) using a highly sensitive sandwich ELISA technique. It is suitable for research involving cell culture supernatants, serum, plasma, and other biological fluids.

Main Applications

• Protein Research
• ELISA Applications (Sandwich Method)
• Functional validation of immunoregulation, antiviral, antibacterial, and immune response mechanisms

Technical Parameters

Analyte	IFN‑γ (Rat)
Detection Range	150 pg/mL – 2400 pg/mL
Sensitivity	15 pg/mL
Sample Type	Cell Culture Supernatant, Serum, Plasma, Other Biological Fluids
Assay Method	Sandwich ELISA (Dual-Antibody Capture-Detection System)

Kit Components

• Pre-coated Microplate with Rat IFN‑γ Antibody
• Rat IFN‑γ Protein Standard
• Rat IFN‑γ Detection Antibody
• EliKine™ Streptavidin-HRP
• Standard Diluent
• Assay Buffer
• HRP Substrate
• Stop Solution
• Wash Buffer
• Plate Sealer

Features & Advantages

• High Sensitivity: Minimum detection limit of 15 pg/mL, meeting the quantitation needs for low-abundance samples.
• High Specificity: Exhibits excellent selectivity for rat IFN‑γ, with no significant cross-reactivity or background interference observed.
• Utilizes the EliKine™ Streptavidin-HRP system for consistent and reliable signal amplification.
• Complete ready-to-use formulations simplify experimental procedures, suitable for high-throughput screening.

Key Operational Notes (Recommended)

• Allow all reagents to equilibrate to room temperature (≈20-25 °C) for 30 minutes before use.
• Use disposable pipette tips throughout the procedure to prevent cross-contamination.
• Reseal unused wells and store at 4 °C.
• Thorough Mixing: Use a low-speed shaker or gently shake manually every 10 minutes.
• It is recommended to run samples and standards in duplicate or triplicate to enhance data reliability.

Storage & Shipping

• Unopened Kit: Store at 2-8 °C.
• After opening, aliquot components according to the instructions for storage.
• Shipping Method: Shipped with ice packs (blue ice) under cold chain conditions to ensure temperature control throughout transit.

Specifications & Pricing

Specification	Tests per Kit	Unit Price (RMB)	Delivery Lead Time
48 T	48 tests	1,598.00	Stock (Ships Next Day)
96 T	96 tests	2,558.00	Stock (Ships Next Day)

Warning & Compliance

For Research Use Only. This product is not intended for human or clinical diagnostic use. Any actions related to patents or licenses conducted using this kit are the sole responsibility of the user; the supplier assumes no liability.

Background

Interferons (IFNs) are critical immune signaling molecules produced by host cells in response to pathogens such as viruses, bacteria, parasites, or tumor cells. Type II interferon IFN‑γ is secreted as a dimer, primarily derived from NK cells, NKT cells, CD4⁺ Th1 cells, and CD8⁺ cytotoxic T cells, playing key roles in both innate and adaptive immunity. IFN‑γ activates macrophages, promotes MHC class II expression, and is closely associated with aberrant expression in various autoinflammatory and autoimmune diseases, making it a common functional marker in immunological research.

Reference Information

• Gene ID: 25712
• Protein ID: P01581 (UniProt)
• Common Aliases: IFG, IFI, IFN‑γ, Interferon gamma, Macrophage Activating Factor, etc.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Yakeyin Company for specific details.