大鼠白介素-2 ELISA定量试剂盒(IL-2)

产品概览

英文名称：Rat IL-2 ELISA Kit
中文名称：大鼠白介素-2 ELISA定量试剂盒 (IL-2)
货号：KTE9002-1
检测对象：大鼠 IL‑2（白介素‑2）
产品分类：核心产品 → 细胞分析 → ELISA

适用范围

本套件专为大鼠来源的细胞培养上清、血清、血浆及其他生物液体中 IL‑2 的定量检测而设计，符合 ELISA（夹心法）实验需求。

一级应用

• ELISA 应用
• 蛋白类分析

检测原理

采用经典的双抗体夹心 ELISA 方案：

1. 将大鼠源 IL‑2 抗体预包覆于 96 孔微孔板上。
2. 加入待测样本或标准品，IL‑2 与捕获抗体特异性结合。
3. 加入标记的检测抗体形成二抗复合物。
4. 加入 EliKine™ Streptavidin‑HRP，HRP 与底物反应产生可比色的信号。
5. 使用终止液终止酶反应后，在酶标仪上读取 450 nm 吸光度，依据标准曲线定量。

试剂盒组成

• 大鼠源 IL‑2 抗体预包被微孔板
• 大鼠源 IL‑2 蛋白标准品（含梯度稀释系列）
• 大鼠源 IL‑2 检测抗体
• EliKine™ Streptavidin‑HRP
• 标准品稀释液
• 实验缓冲液
• HRP 底物（TMB）
• 反应终止液
• 洗涤缓冲液
• 封板膜

关键特点 & 优势

• 高灵敏度与特异性，能够在复杂基质中准确捕获 IL‑2。
• 经验证对同类细胞因子（如 IL‑1、TNF‑α）无明显交叉反应，背景噪声低。
• 配套的预包被板和标准品保证实验一致性，适合研发实验室及质量控制中心大批量使用。

检测范围 & 灵敏度

本产品的具体检出范围与灵敏度请参照说明书中的实验验证数据（当前信息暂缺）。

适用样本类型

细胞培养上清、血清、血浆以及其他生物液体。

规格与定价

规格	单价 (¥)	货期	备注
48 条	1598.0	期货（4 天内发货）	适合小规模实验
96 条	2558.0	期货（4 天内发货）	性价比更高的批量采购

储存与运输

• 未开封试剂盒请存放于 2–8 ℃ 冰箱。
• 启用后请根据说明书分别保存各组分（多数组分在 2–8 ℃，底物类可放于 -20 ℃）。
• 运输采用蓝冰（冷藏）包装，确保全程低温。

使用注意事项

• 同一批次内的试剂方可混用，避免跨批次或过期试剂混合。
• 使用前请让所有试剂在室温平衡 30 分钟。
• 实验过程中务必使用一次性吸头，以防交叉污染。
• 未使用的板条请立即放回包装，密闭保存于 4 ℃。
• 混匀对实验结果尤为重要，建议使用低速振荡器或每 10 分钟手动轻摇一次。
• 样品及标准品建议采用双孔或三孔复孔检测，提高数据可靠性。

警告 & 合规声明

本试剂盒仅供科研使用，不适用于人类或临床诊断。使用本品进行的任何专利或许可证相关的行为风险均由使用者自行承担，本公司不承担相应责任。

产品背景

白细胞介素‑2（IL‑2）是一种 15 kDa 的糖蛋白，编码基因为 GeneID 116562（位于人类染色体 4q26–28），在免疫调节中起关键作用，促进 T 细胞增殖与分化。大鼠 IL‑2 与人类 IL‑2 在序列上保守，可用于免疫学研究、药理学筛选以及细胞功能评估。

更多蛋白信息请访问 UniProt – P17108。

检索关键字

白介素‑2 ELISA 试剂盒、Rat IL‑2 ELISA Kit、IL‑2、TCGF、T cell growth factor、aldesleukin、KTE9002-1、KET9002

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Rat IL-2 ELISA Kit
Chinese Name: Rat Interleukin-2 ELISA Quantitative Kit (IL-2)
Catalog Number: KTE9002-1
Detection Target: Rat IL-2 (Interleukin-2)
Product Category: Core Products → Cell Analysis → ELISA

Intended Use

This kit is specifically designed for the quantitative detection of IL-2 in rat-derived cell culture supernatants, serum, plasma, and other biological fluids, meeting the requirements for ELISA (Sandwich Method) experiments.

Primary Applications

• ELISA Application
• Protein Analysis

Detection Principle

Utilizes the classic double-antibody sandwich ELISA protocol:

1. Rat IL-2 antibody is pre-coated onto the 96-well microplate.
2. Add test samples or standards; IL-2 binds specifically to the capture antibody.
3. Add the labeled detection antibody to form an antibody-antigen-antibody complex.
4. Add EliKine™ Streptavidin-HRP; HRP reacts with the substrate to produce a colorimetric signal.
5. After adding the stop solution to terminate the enzyme reaction, measure the absorbance at 450 nm using a microplate reader and quantify based on the standard curve.

Kit Components

• Pre-coated microplate with rat IL-2 antibody
• Rat IL-2 protein standard (including serial dilutions)
• Rat IL-2 detection antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate (TMB)
• Stop solution
• Wash buffer
• Plate sealer

Key Features & Advantages

• High sensitivity and specificity enable accurate capture of IL-2 in complex matrices.
• Validated for minimal cross-reactivity with related cytokines (e.g., IL-1, TNF-α), resulting in low background noise.
• Pre-coated plates and matched standards ensure experimental consistency, suitable for R&D laboratories and quality control centers requiring high-throughput analysis.

Detection Range & Sensitivity

Please refer to the experimental validation data in the instruction manual for the specific detection range and sensitivity of this product (current information is temporarily unavailable).

Compatible Sample Types

Cell culture supernatants, serum, plasma, and other biological fluids.

Specifications & Pricing

Specification	Unit Price (¥)	Lead Time	Remarks
48 tests	1598.0	Stock (ships within 4 days)	Suitable for small-scale experiments
96 tests	2558.0	Stock (ships within 4 days)	Cost-effective for bulk purchases

Storage & Shipping

• Store the unopened kit at 2–8 °C.
• After opening, store individual components separately as per the manual (most components at 2–8 °C; substrates can be stored at -20 °C).
• Shipped with blue ice (refrigerated) packaging to ensure consistent low temperature during transit.

Precautions for Use

• Use reagents only from the same kit lot; avoid mixing reagents from different lots or expired reagents.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable pipette tips throughout the assay to prevent cross-contamination.
• Return unused strips to their pouch immediately and store sealed at 4 °C.
• Thorough mixing is critical; use a low-speed shaker or gently shake manually every 10 minutes.
• It is recommended to test samples and standards in duplicate or triplicate to improve data reliability.

Warning & Compliance Statement

This kit is for research use only. Not intended for human or clinical diagnostic use. The user assumes all risks associated with any patent or licensing activities related to the use of this product. The company bears no corresponding responsibility.

Product Background

Interleukin-2 (IL-2) is a 15 kDa glycoprotein, encoded by the gene GeneID 116562 (located on human chromosome 4q26–28). It plays a key role in immune regulation, promoting T cell proliferation and differentiation. Rat IL-2 is conserved in sequence with human IL-2 and is useful for immunology research, pharmacological screening, and cell function assessment.

For more protein information, visit UniProt – P17108.

Search Keywords

Interleukin-2 ELISA Kit, Rat IL-2 ELISA Kit, IL-2, TCGF, T cell growth factor, aldesleukin, KTE9002-1, KET9002

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.