CCL4/MIP-1 beta ELISA KIT 趋化因子

人CCL4/MIP-1 beta ELISA试剂盒：高灵敏度的趋化因子定量分析解决方案

本试剂盒是一款基于双抗体夹心法原理开发的优质ELISA检测产品，专门用于精准定量人血清、血浆或细胞培养上清样本中的趋化因子配体4 (CCL4/MIP-1β)浓度。作为EliKine™系列核心产品，其设计旨在为免疫学、炎症研究和相关疾病机制探索提供可靠、重复性高的检测数据。

产品核心优势

• 高灵敏度与特异性：最低检测浓度达15.63 pg/mL，检测范围为15.63–1000 pg/mL，并对目标蛋白类似物无明显交叉反应。
• 稳定可靠的性能：试剂盒组分经过严格优化和质控，确保批间一致性，降低实验误差。
• 灵活的样本兼容性：适用于多种生物样本类型，包括细胞培养上清、血清和血浆。
• 即用型试剂配置：提供预包被微孔板、冻干标准品、检测抗体及全套缓冲液，简化实验流程。

试剂盒组分详情

组分名称	规格	状态/说明
Human CCL4 Microplate	1块	预包被微孔板
Human CCL4 Standard (lyophilized)	2支	冻干标准品
Standard Diluent & Assay Buffer (10×)	各1瓶	即用型缓冲液
Human CCL4 Detect Antibody (100×)	1支	生物素标记检测抗体
Streptavidin-HRP (100×) & HRP Substrate (TMB)	各1瓶	信号放大与显色系统
Stop Solution & Wash Buffer (20×)	各1瓶	终止液与洗涤液
Plate Covers	2张	封板膜

技术参数一览

• 产品货号：KTE3113-2
• 反应性：人
• 检测方法：夹心法ELISA
• 灵敏度：15.63 pg/mL
• 检测范围：15.63–1000 pg/mL
• 样本类型：细胞培养上清、血清、血浆

背景与应用价值

CCL4（巨噬细胞炎性蛋白-1β）是一种重要的CC趋化因子，通过特异性结合CCR5受体，参与调控自然杀伤细胞、单核细胞等免疫细胞的趋化活动。其在炎症反应、感染性疾病（如HIV抑制机制）及免疫调节研究中具有关键作用。本试剂盒通过高度特异性的抗体对，实现对生物样本中CCL4的精准定量，助力科研人员深入探索其在病理生理过程中的功能。

规格与订购信息

规格	价格（元）	货期
48 T	1598.0	请咨询
96 T	2558.0	期货（5天内发货）

保存与运输

• 保存条件：未开封试剂盒避光保存于2–8℃，有效期24个月；启用后请参照说明书保存各组分。
• 运输方式：冰袋（蓝冰）运输，确保试剂活性。

重要注意事项

• 请勿混合不同批号或过期试剂；使用前需室温平衡30分钟。
• 推荐使用一次性吸头，避免交叉污染；未用的板条需密封保存于4℃。
• 实验过程中建议对样品和标准品进行复孔检测，并通过振荡充分混匀。
• 本产品仅用于科学研究，不适用于临床诊断。

如需了解更多技术细节或订购支持，请联系我们的产品顾问获取信息。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human CCL4/MIP-1 beta ELISA Kit: High-Sensitivity Chemokine Quantitative Analysis Solution

This kit is a high-quality ELISA detection product developed based on the double antibody sandwich method, specifically designed for the accurate quantification of chemokine ligand 4 (CCL4/MIP-1β) concentration in human serum, plasma, or cell culture supernatant samples. As a core product of the EliKine™ series, it is designed to provide reliable and highly reproducible detection data for immunology, inflammation research, and exploration of related disease mechanisms.

Core Product Advantages

• High Sensitivity and Specificity: Minimum detectable concentration reaches 15.63 pg/mL, with a detection range of 15.63–1000 pg/mL, and shows no significant cross-reactivity with analogous proteins.
• Stable and Reliable Performance: Kit components undergo rigorous optimization and quality control, ensuring lot-to-lot consistency and reducing experimental errors.
• Flexible Sample Compatibility: Suitable for various biological sample types, including cell culture supernatant, serum, and plasma.
• Ready-to-Use Reagent Configuration: Provides pre-coated microplates, lyophilized standards, detection antibodies, and a full set of buffers to simplify the experimental procedure.

Kit Component Details

Component Name	Specification	Status/Description
Human CCL4 Microplate	1 plate	Pre-coated microplate
Human CCL4 Standard (lyophilized)	2 vials	Lyophilized standard
Standard Diluent & Assay Buffer (10×)	1 bottle each	Ready-to-use buffers
Human CCL4 Detect Antibody (100×)	1 vial	Biotin-labeled detection antibody
Streptavidin-HRP (100×) & HRP Substrate (TMB)	1 bottle each	Signal amplification and chromogenic system
Stop Solution & Wash Buffer (20×)	1 bottle each	Stop solution and wash buffer
Plate Covers	2 sheets	Plate sealer

Technical Parameters Overview

• Catalog Number: KTE3113-2
• Reactivity: Human
• Detection Method: Sandwich ELISA
• Sensitivity: 15.63 pg/mL
• Detection Range: 15.63–1000 pg/mL
• Sample Types: Cell culture supernatant, serum, plasma

Background and Application Value

CCL4 (Macrophage Inflammatory Protein-1 beta) is an important CC chemokine that participates in regulating the chemotactic activity of immune cells such as natural killer cells and monocytes by specifically binding to the CCR5 receptor. It plays a key role in inflammatory responses, infectious diseases (such as HIV inhibition mechanisms), and immunoregulation research. This kit enables precise quantification of CCL4 in biological samples through highly specific antibody pairs, assisting researchers in exploring its function in pathophysiological processes.

Specifications and Ordering Information

Specification	Price (RMB)	Lead Time
48 T	1598.0	Please inquire
96 T	2558.0	Future goods (shipped within 5 days)

Storage and Transportation

• Storage Conditions: Unopened kit should be stored protected from light at 2–8°C, valid for 24 months; after opening, please store components according to the instructions.
• Transportation Method: Shipped with ice packs (blue ice) to ensure reagent activity.

Important Notes

• Do not mix reagents from different lots or use expired reagents; allow reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable tips to avoid cross-contamination; unused strips should be sealed and stored at 4°C.
• It is recommended to perform duplicate tests for samples and standards during the experiment, and mix thoroughly by shaking.
• This product is for research use only, not for clinical diagnosis.

For more technical details or ordering support, please contact our product consultant for the latest information.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact YaKeyin Company for specific details.