CCL17/TARC ELISA KIT 趋化因子17ELI

产品概述

英文名称：Human CCL17/TARC ELISA Kit
中文名称：CCL17/TARC ELISA KIT 趋化因子 17 ELISA 试剂盒（人）
货号：KTE3110-2
产品分类：核心产品 → 细胞分析 → ELISA（夹心法）

适用领域

• 蛋白类研究
• ELISA 应用（定量检测）
• 细胞培养上清、血清、血浆等液体样本

检测原理

本套件基于双抗体夹心法（Sandwich ELISA），使用特异性包被抗体捕获样本中人 CCL17/TARC，随后加入生物素化检测抗体与链霉亲和素‑HRP 复合物形成免疫复合体，最后通过 TMB 底物显色并在 450 nm 读取 OD 值，实现定量测定。

主要成分

• Human CCL17 Microplate（96 孔酶标板）
• Human CCL17 Standard (lyophilized) – 提供完整标准曲线
• Standard Diluent
• Assay Buffer (10×)
• Human CCL17 Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

性能指标

检测范围	15.63 pg/mL ~ 1,000 pg/mL
灵敏度 (LOD)	15.63 pg/mL
特异性	未检测到显著的交叉反应或背景干扰
样本类型	细胞培养上清、血清、血浆
读取波长	450 nm（双波长模式可选 620 nm 参考）

使用提示 & 注意事项

• 试剂请勿与其他批号或超过有效期的试剂混用。
• 使用前于室温平衡 30 分钟。
• 实验全程建议使用一次性吸头，防止交叉污染。
• 未使用的酶标板需及时放回原包装，密封后在 4 ℃保存。
• 洗涤和混匀步骤对结果影响显著，推荐使用低速振荡器或每 10 分钟手动轻摇一次。
• 样品及标准品建议采用双孔或三孔复孔设置，以提高数据可靠性。

保存与运输

• 未开封试剂盒：避光保存于 2 ℃ ~ 8 ℃，保质期 24 个月。
• 已开封各组分请参考随盒说明书的存放条件。
• 运输采用蓝色冰袋冷链模式，确保全程 2 ℃ ~ 8 ℃。

包装规格 & 报价

规格	含量	单价（人民币）	货期
48 T	48 套	1,598.00	期货（5 天内发货）
96 T	96 套	2,558.00	期货（5 天内发货）

法规声明 & 警告

本产品仅限用于科研目的，不适用于临床诊断或人类治疗。使用本套件不得违反任何专利或许可证，因侵权或其他违规行为产生的后果由使用者自行承担。

技术背景

趋化因子 17（CCL17/TARC）是胸腺活化调节趋化因子，属于 C‑C 趋化因子家族，主要通过 CCR4 受体介导 T 细胞的趋化。在免疫调节、炎症及肿瘤微环境研究中，CCL17 的定量检测常用于评估细胞因子谱及信号通路活性。本套件提供了高灵敏度、低背景的定量平台，帮助实验室快速获取可靠数据。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Human CCL17/TARC ELISA Kit
Chinese Name: CCL17/TARC ELISA KIT Chemokine 17 ELISA Reagents (Human)
Catalog No.: KTE3110-2
Product Category: Core Products → Cell Analysis → ELISA (Sandwich)

Applicable Fields

• Protein research
• ELISA applications (quantitative detection)
• Cell culture supernatants, serum, plasma and other liquid specimens

Assay Principle

This kit utilizes a double‑antibody sandwich ELISA. A capture antibody coated on the plate specifically binds human CCL17/TARC in the sample. A biotinylated detection antibody and streptavidin‑HRP conjugate then form an immune complex. Color development is achieved with TMB substrate and optical density is measured at 450 nm for quantitative determination.

Key Components

• Human CCL17 Microplate (96‑well plate)
• Human CCL17 Standard (lyophilized) – provides a full standard curve
• Standard Diluent
• Assay Buffer (10×)
• Human CCL17 Detection Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Performance Specifications

Detection Range	15.63 pg/mL ~ 1,000 pg/mL
Sensitivity (LOD)	15.63 pg/mL
Specificity	No significant cross‑reactivity or background interference detected
Sample Types	Cell culture supernatant, serum, plasma
Read Wavelength	450 nm (dual‑wavelength mode optional at 620 nm reference)

Usage Tips & Precautions

• Do not mix reagents with different lot numbers or with expired reagents.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable pipette tips throughout the assay to avoid cross‑contamination.
• Unutilized microplates should be returned to their original packaging, sealed, and stored at 4 °C.
• Washing and mixing steps significantly affect results; recommend a low‑speed orbital shaker or gentle manual inversion every 10 minutes.
• Employ duplicate or triplicate well layouts for samples and standards to increase data reliability.

Storage & Shipping

• Unopened kit: store protected from light at 2 °C ~ 8 °C; shelf life 24 months.
• Opened components: follow the storage conditions described in the accompanying manual.
• Shipping is performed with blue ice packs in a cold‑chain to maintain 2 °C ~ 8 °C throughout transit.

Package Specifications & Pricing

Specification	Quantity	Unit Price (CNY)	Lead Time
48 T	48 kits	1,598.00	In stock (shipment within 5 days)
96 T	96 kits	2,558.00	In stock (shipment within 5 days)

Regulatory Statement & Warning

This product is intended for research use only and is not suitable for clinical diagnosis or human therapy. Use of this kit must not infringe any patents or licenses; any liability arising from infringement or other non‑compliant activities is the sole responsibility of the user.

Technical Background

Chemokine 17 (CCL17/TARC) is a thymus‑activated regulatory chemokine belonging to the C‑C chemokine family and mediates T‑cell chemotaxis primarily through the CCR4 receptor. Quantitative measurement of CCL17 is widely employed in immunoregulation, inflammation, and tumor microenvironment studies to assess cytokine profiles and signaling pathway activity. This kit offers a high‑sensitivity, low‑background quantitative platform, enabling laboratories to obtain reliable data rapidly.

Note: The information above may change with product optimization or upgrades. For the most current details, please contact Yakoin Co., Ltd.