人白介素2受体 ELISA定量试剂盒(sIL-2Rα)

EliKine™ 人白介素2受体α (sIL-2Rα/CD25) ELISA定量试剂盒

货号：KTE3046-1 | 产品中心：细胞分析 | 产品分类：核心产品

产品概述

EliKine™ Human sIL-2Rα/CD25 ELISA Kit是一款基于经典双抗体夹心法原理开发的优质试剂盒，专门用于精准定量检测人血清、血浆或细胞培养上清样本中可溶性白细胞介素-2受体α (sIL-2Rα)的浓度。本试剂盒凭借其出色的高灵敏度和高特异性，成为免疫学研究、疾病机制探索及生物标志物分析的可靠工具。

主要特点与优势

• 卓越的性能指标：检测范围宽广，达78.13 pg/mL - 5,000 pg/mL；灵敏度高，最低可检测至5.76 pg/mL。
• 高度的特异性：经过严格验证，对目标蛋白的类似物无明显交叉反应或背景干扰，确保检测结果的准确性。
• 操作流程优化：试剂盒组分经过精心配比和优化，提供清晰的实验方案，有助于获得稳定且可重复的实验结果。
• 灵活的规格选择：提供48孔与96孔两种规格，满足不同通量的实验需求。

试剂盒组分（详细清单）

• 预包被微孔板 (Human sIL-2Rα Microplate)
• 冻干标准品 (Human sIL-2Rα Standard, lyophilized)
• 标准品稀释液 (Standard Diluent)
• 10倍浓缩检测缓冲液 (Assay Buffer 10×)
• 100倍浓缩生物素化检测抗体 (Human sIL-2Rα Detect Antibody 100×)
• 100倍浓缩链霉亲和素-HRP (Streptavidin-HRP 100×)
• TMB显色底物 (HRP Substrate)
• 终止液 (Stop Solution)
• 20倍浓缩洗涤液 (Wash Buffer 20×)
• 板盖 (Plate Covers)

背景信息

可溶性白细胞介素-2受体α (sIL-2Rα/CD25)是免疫调节中的重要分子。其水平变化与T细胞、B细胞的活化状态密切相关，是反映机体免疫系统激活程度的关键指标。在类风湿性关节炎、系统性红斑狼疮、某些类型的白血病和淋巴瘤等疾病的研究中，监测sIL-2Rα的浓度变化能为病理状态提供有价值的见解。

技术参数

参数	详情
反应种属	人 (Human)
样本类型	细胞培养上清、血清、血浆
检测方法	夹心法ELISA
保存条件	未开封试剂盒请避光保存于2-8℃，保质期24个月
运输条件	冰袋（蓝冰）运输

订购信息

规格	价格 (人民币)	货期
48 T	1,598.0	期货（预计5个工作日内发货）
96 T	2,558.0	期货（预计5个工作日内发货）

重要注意事项

• 请勿混合使用不同批号的试剂或已过有效期的试剂。
• 使用前，所有试剂应在室温下平衡约30分钟。
• 为避免交叉污染，实验过程中请务必使用一次性吸头。
• 未使用的板条需立即放回原包装袋并密封，于4℃保存。
• 充分的混匀对实验结果至关重要，建议使用低速振荡器或定期手动摇匀。
• 为获得更可靠的数据，建议对样品和标准品进行双孔或三孔复孔检测。

警告：本产品仅供科研使用，不适用于人类或临床诊断。使用本产品可能受相关专利许可限制，请使用者确保其应用符合当地法律法规。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Human Interleukin‑2 Receptor α (sIL‑2Rα/CD25) ELISA Quantitation Kit

Catalog No.: KTE3046-1 | Product Center: Cell Analysis | Product Category: Core Product

Product Overview

The EliKine™ Human sIL‑2Rα/CD25 ELISA Kit is a high‑quality reagent kit developed on the classic sandwich ELISA principle, specifically designed for precise quantitative measurement of soluble interleukin‑2 receptor α (sIL‑2Rα) concentrations in human serum, plasma, or cell‑culture supernatant samples. This kit offers outstanding high sensitivity and high specificity, making it a reliable tool for immunology research, disease‑mechanism studies, and biomarker analysis.

Key Features and Advantages

• Exceptional performance metrics: Broad detection range of 78.13 pg/mL – 5,000 pg/mL; high sensitivity with a lower limit of detection of 5.76 pg/mL.
• High specificity: Rigorously validated to exhibit negligible cross‑reactivity or background interference with protein homologs, ensuring accurate results.
• Optimized workflow: Reagents are precisely formulated and optimized, providing a clear experimental protocol that yields stable and reproducible data.
• Flexible format options: Available in 48‑well and 96‑well formats to accommodate varying throughput needs.

Kit Components (Detailed List)

• Pre‑coated microplate (Human sIL‑2Rα Microplate)
• Lyophilized standard (Human sIL‑2Rα Standard, lyophilized)
• Standard Diluent
• Assay Buffer 10×
• Human sIL‑2Rα Detect Antibody 100× (biotinylated)
• Streptavidin‑HRP 100×
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer 20×
• Plate Covers

Background Information

Soluble interleukin‑2 receptor α (sIL‑2Rα/CD25) is a pivotal molecule in immune regulation. Its concentration correlates closely with the activation status of T and B lymphocytes and serves as a key indicator of overall immune system activation. Monitoring sIL‑2Rα levels provides valuable insights into pathological states in diseases such as rheumatoid arthritis, systemic lupus erythematosus, certain leukemias, and lymphomas.

Technical Specifications

Parameter	Details
Species Reactivity	Human
Sample Types	Cell‑culture supernatant, serum, plasma
Assay Method	Sandwich ELISA
Storage Conditions	Unopened kits should be stored protected from light at 2‑8 °C; shelf‑life 24 months
Shipping Conditions	Transport with ice packs (blue ice)

Ordering Information

Format	Price (CNY)	Lead Time
48 T	1,598.0	Futures (estimated shipment within 5 business days)
96 T	2,558.0	Futures (estimated shipment within 5 business days)

Critical Precautions

• Avoid mixing reagents from different lots or using expired reagents.
• Allow all reagents to equilibrate to room temperature for approximately 30 minutes before use.
• Use disposable pipette tips throughout the assay to prevent cross‑contamination.
• Unutilized plates should be returned to their original packaging, sealed, and stored at 4 °C.
• Thorough mixing is essential for reliable results; low‑speed vortexing or periodic manual agitation is recommended.
• For increased data robustness, perform duplicate or triplicate measurements of samples and standards.

Warning: This product is intended for research use only and is not suitable for human or clinical diagnostic applications. Use of this product may be subject to patent licensing restrictions; users are responsible for ensuring compliance with applicable laws and regulations.

Note: The information above may be updated as product improvements occur. For the most current details, please contact EliKine.