TIM-1/KIM-1/HAVCR ELISA KIT T细

产品概述

货号：KTE3068-2

中文名称：人T细胞免疫球蛋白域粘蛋白域蛋白-1 (TIM-1/KIM-1/HAVCR) ELISA试剂盒

英文名称：Human TIM-1/KIM-1/HAVCR ELISA Kit

本产品是EliKine™系列中的核心产品，专为人源样本中TIM-1蛋白的精确定量检测而设计。采用经典的夹心法ELISA原理，适用于细胞培养上清、血清、血浆等多种样本类型，为免疫学研究、疾病机制探索及生物标志物分析提供可靠的工具。

主要特性与优势

• 高灵敏度：检测下限低至31.25 pg/mL，能够有效检测样本中低丰度的TIM-1蛋白。
• 优异特异性：经过严格验证，对目标蛋白的类似物未观察到明显的交叉反应或背景干扰，确保检测结果的准确性。
• 宽检测范围：定量范围覆盖31.25 pg/mL至2,000 pg/mL，满足不同浓度样本的检测需求。
• 操作便捷：试剂盒提供完整的预包被微孔板及优化好的缓冲液体系，简化实验流程。

试剂盒组分

• Human TIM-1 Microplate (预包被微孔板)
• Human TIM-1 Standard (冻干标准品)
• Assay Buffer (10×)
• Human TIM-1 Detect Antibody (100×，生物素化检测抗体)
• Streptavidin-HRP (100×，链霉亲和素-HRP偶联物)
• HRP Substrate (TMB，显色底物)
• Stop Solution (终止液)
• Wash Buffer (20×，洗涤缓冲液)
• Plate Covers (板盖)

注：具体组分含量请参考随货说明书。

技术参数

产品规格与价格

背景信息

T细胞免疫球蛋白域粘蛋白域蛋白-1 (TIM-1)，也被称为肾损伤因子-1 (KIM-1) 或甲型肝炎病毒细胞受体1 (HAVcr1)，是TIM基因家族的重要成员。它在调控免疫细胞活性，特别是对病毒感染的宿主免疫应答中扮演着关键角色。TIM-1优先表达于Th2细胞，作为T细胞活化的刺激分子，并参与过敏反应、哮喘及移植耐受等病理生理过程。值得注意的是，在人类中，TIM-1可作为甲型肝炎病毒、登革病毒等多种病毒进入细胞的受体，使其成为感染性疾病研究中的重要靶点。

实验注意事项

• 请勿将不同批号的试剂或过期试剂混合使用。
• 使用前，所有试剂应在室温下平衡约30分钟。
• 为避免交叉污染，实验过程中请使用一次性吸头。
• 未使用的板条应立即放回原包装袋并密封，于4℃保存。
• 充分的混匀对实验结果至关重要，建议使用低速振荡器或定期手动摇匀。
• 为获得更可靠的数据，建议对样品和标准品进行复孔（双孔或三孔）检测。

警告：本产品仅供研究使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Catalog No.:KTE3068-2

Chinese Name: Human T‑cell Immunoglobulin and Mucin Domain‑1 (TIM‑1/KIM‑1/HAVCR) ELISA Kit

English Name: Human TIM‑1/KIM‑1/HAVCR ELISA Kit

This product is a core component of the EliKine™ series, specifically designed for precise quantitative detection of TIM‑1 protein in human-derived samples. Utilizing the classic sandwich‑ELISA principle, it is suitable for a variety of sample types including cell culture supernatants, serum, and plasma, providing a reliable tool for immunology research, disease mechanism investigation, and biomarker analysis.

Key Features and Advantages

• High Sensitivity: Lower detection limit of 31.25 pg/mL, enabling effective detection of low‑abundance TIM‑1 protein in samples.
• Excellent Specificity: Rigorously validated; no significant cross‑reaction or background interference with protein analogs, ensuring accurate results.
• Wide Dynamic Range: Quantitative range spanning 31.25 pg/mL to 2,000 pg/mL, accommodating samples with varying concentrations.
• User‑Friendly Operation: Kit includes a fully pre‑coated microplate and optimized buffer system, streamlining the workflow.

Kit Components

• Human TIM‑1 Microplate (pre‑coated microplate)
• Human TIM‑1 Standard (lyophilized standard)
• Assay Buffer (10×)
• Human TIM‑1 Detect Antibody (100×, biotinylated detection antibody)
• Streptavidin‑HRP (100×, streptavidin‑HRP conjugate)
• HRP Substrate (TMB, chromogenic substrate)
• Stop Solution (termination solution)
• Wash Buffer (20×, washing buffer)
• Plate Covers (plate seals)

Note: Refer to the accompanying instructions for detailed component quantities.

Technical Specifications

Product Specifications and Pricing

Background Information

T‑cell immunoglobulin and mucin domain‑1 (TIM‑1), also known as kidney injury molecule‑1 (KIM‑1) or hepatitis A virus cellular receptor 1 (HAVcr1), is a key member of the TIM gene family. It plays a pivotal role in regulating immune cell activity, particularly in host immune responses to viral infections. TIM‑1 is preferentially expressed on Th2 cells, functioning as a co‑stimulatory molecule for T‑cell activation and is involved in allergic reactions, asthma, and transplant tolerance. Notably, in humans, TIM‑1 serves as a cellular receptor for multiple viruses, including hepatitis A virus, dengue virus, and Ebola virus, rendering it an important target in infectious disease research.

Experimental Considerations

• Do not mix reagents from different batches or use expired reagents.
• Allow all reagents to equilibrate at room temperature for approximately 30 minutes before use.
• Use disposable pipette tips throughout the experiment to avoid cross‑contamination.
• Unutilized strips should be promptly returned to their original packaging, sealed, and stored at 4 °C.
• Thorough mixing is critical for reliable results; use a low‑speed shaker or manually invert periodically.
• For enhanced data reliability, perform replicate wells (duplicate or triplicate) for samples and standards.

Warning: This product is for research use only and is not intended for human or clinical diagnostic applications. Users must ensure that their use does not infringe any patents or licensing agreements.

Note: The above information is subject to change due to product optimization or upgrades; please contact 亚科因公司 for the most current details.