IL-33 ELISA KIT 白介素33ELISA试剂盒(

产品概览 – Human IL-33 ELISA Kit (KTE3058-2)

中文名称：IL-33 ELISA KIT 白介素33 ELISA 试剂盒（人）
英文名称：Human IL-33 ELISA Kit
产品分类：核心产品 | 细胞分析平台 | ELISA 夹心法检测

适用领域

• 蛋白类分析 – 重点用于 IL-33 的定量检测
• 一级应用：ELISA（酶联免疫吸附测定）
• 样本类型：细胞培养上清、血清、血浆
• 研究用途：细胞信号通路、炎症因子、免疫调节等基础与药理研究

检测概述

本套件采用 EliKine™ 双抗体夹心法，通过固相抗体捕获样品中的人 IL-33，再利用生物素化检测抗体、链霉亲和素‑HRP 以及 TMB 显色剂实现酶标板上 450 nm 读取的定量。检测范围 31.25 pg/mL – 2,000 pg/mL，检出限（灵敏度）为 31.25 pg/mL。

关键优势

• 极高灵敏度与特异性：针对 IL-33 设计的捕获与检测抗体在交叉反应测试中未出现明显干扰。
• 宽广线性范围：31.25 pg/mL‑2,000 pg/mL，覆盖多数实验需求。
• 标准化试剂组合：全部关键试剂均为预配比，减少配制误差。
• 兼容常规仪器：适用于市面上主流酶标仪（450 nm）进行光密度读取。

试剂盒组成（每套）

• Human IL-33 Microplate（预包被）
• Human IL-33 Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Human IL-33 Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

推荐实验步骤（概览）

1. 将微孔板放置于室温平衡 30 分钟。
2. 加入 50 µL–100 µL 样本或标准品至每孔。
3. 孵育 1–2 小时（或依据说明书），随后用 20× Wash Buffer 洗涤 3 次。
4. 加入 100 µL 检测抗体（100× 稀释）并孵育 1 小时。
5. 洗涤后加入 100 µL 链霉亲和素‑HRP（100×）孵育 30 分钟。
6. 再洗涤后加入 100 µL TMB 底物，显色 10–20 分钟。
7. 加入 50 µL 停止液，读取 450 nm OD 值。

建议使用双孔或三孔复孔设置，以提高结果的重复性。

技术参数表

检测指标	IL-33
检测范围	31.25 pg/mL – 2,000 pg/mL
灵敏度	31.25 pg/mL
样本类型	细胞培养上清、血清、血浆
反应性	人 (Human)
检测方法	ELISA（双抗体夹心法）

包装规格 & 价格

规格	价格 (CNY)	交货期
48 T	1,598.00	请咨询
96 T	2,558.00	期货（5 天内发货）

储存与运输

• 未开封：避光保存于 2–8 ℃，有效期 24 个月。
• 开启后：请参照说明书分别保存各组分（多数组分建议 4 ℃，避免反复冻融）。
• 运输条件：使用蓝冰（冷链）包装，确保全程低温。

使用注意事项

• 不同批号或过期的试剂不可混用。
• 使用一次性吸头防止交叉污染。
• 未使用的板条请立即放回包装，密闭保存于 4 ℃。
• 充分混匀对结果至关重要，推荐低速振荡或每10 分钟手动轻摇。
• 样品和标准建议做双孔或三孔复孔检测。

警示声明

本产品仅供科研使用，不适用于人类或临床诊断。使用本套件进行任何专利或许可证受限的实验，需自行确保合规，制造商不承担相应法律责任。

产品背景

白细胞介素 33（IL-33）是 IL-1 家族成员，分子量约 30 kDa，由成纤维细胞、肥大细胞、树突细胞、巨噬细胞、成骨细胞、内皮细胞和上皮细胞等多种细胞分泌。IL-33 通过与受体 ST2 及 IL‑1RAP 结合，激活 NF‑κB 与 MAPK 信号通路，诱导 Th2 细胞分泌 IL-5、IL-13 等 II 型细胞因子，在粘膜免疫、炎症及过敏等多种生理病理过程中发挥关键作用。对 IL‑33 的精准定量，是解析其生物学功能及评价药物干预效果的重要手段。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview – Human IL-33 ELISA Kit (KTE3058-2)

Chinese Name: IL-33 ELISA KIT 白介素33 ELISA 试剂盒（人）
English Name: Human IL-33 ELISA Kit
Product Category: Core Products | Cell Analysis Platform | ELISA Sandwich Assay

Application

• Protein Analysis – Specifically designed for quantitative detection of IL-33
• Primary Application: ELISA (Enzyme-Linked Immunosorbent Assay)
• Sample Types: Cell culture supernatant, serum, plasma
• Research Use: Basic and pharmacological research in cell signaling pathways, inflammatory factors, immune regulation, etc.

Assay Principle

This kit employs the EliKine™ Double-Antibody Sandwich method. Human IL-33 in samples is captured by a solid-phase antibody, followed by detection using a biotinylated detection antibody, streptavidin-HRP, and TMB substrate for colorimetric quantification read at 450 nm on a microplate reader. The detection range is 31.25 pg/mL – 2,000 pg/mL, with a sensitivity (limit of detection) of 31.25 pg/mL.

Key Advantages

• High Sensitivity and Specificity: Capture and detection antibodies specifically designed for IL-33 show no significant cross-reactivity in interference tests.
• Wide Linear Range: 31.25 pg/mL – 2,000 pg/mL, covering most experimental requirements.
• Standardized Reagent Set: All key reagents are pre-formulated and pre-diluted, minimizing preparation errors.
• Compatibility with Standard Instruments: Suitable for use with common microplate readers (450 nm).

Kit Components (per kit)

• Human IL-33 Microplate (pre-coated)
• Human IL-33 Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Human IL-33 Detect Antibody (100×)
• Streptavidin-HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Recommended Procedure (Overview)

1. Allow the microplate to equilibrate at room temperature for 30 minutes.
2. Add 50 µL – 100 µL of sample or standard to each well.
3. Incubate for 1 – 2 hours (or as per instructions), then wash 3 times using diluted 20× Wash Buffer.
4. Add 100 µL of Detection Antibody (diluted 100×) and incubate for 1 hour.
5. After washing, add 100 µL of Streptavidin-HRP (diluted 100×) and incubate for 30 minutes.
6. After washing again, add 100 µL of TMB Substrate and develop color for 10 – 20 minutes.
7. Add 50 µL of Stop Solution and read the Optical Density (OD) at 450 nm.

It is recommended to use duplicate or triplicate wells for samples and standards to improve result reproducibility.

Technical Parameters

Analyte	IL-33
Detection Range	31.25 pg/mL – 2,000 pg/mL
Sensitivity	31.25 pg/mL
Sample Types	Cell culture supernatant, serum, plasma
Reactivity	Human
Assay Method	ELISA (Double-Antibody Sandwich)

Packaging & Price

Size	Price (CNY)	Delivery Time
48 T	1,598.00	Please inquire
96 T	2,558.00	Futures (Ships within 5 days)

Storage & Shipping

• Unopened: Store protected from light at 2–8 ℃; shelf life is 24 months.
• After Opening: Refer to the manual for storage of individual components (most components should be stored at 4 ℃; avoid repeated freeze-thaw cycles).
• Shipping Conditions: Shipped with blue ice (cold chain packaging) to maintain low temperature.

Precautions for Use

• Do not mix reagents from different lots or expired reagents.
• Use disposable pipette tips to prevent cross-contamination.
• Return unused strips to their original pouch immediately, seal, and store at 4 ℃.
• Thorough mixing is crucial; low-speed shaking or manual gentle shaking every 10 minutes is recommended.
• It is recommended to test samples and standards in duplicate or triplicate.

Warning Statement

This product is for research use only. Not intended for human or clinical diagnostic use. Users conducting experiments subject to patent or license restrictions are responsible for ensuring compliance; the manufacturer assumes no corresponding legal liability.

Product Background

Interleukin-33 (IL-33) is a member of the IL-1 family with a molecular weight of approximately 30 kDa. It is secreted by various cell types including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells. IL-33 binds to its receptor ST2 and IL-1RAP, activating NF-κB and MAPK signaling pathways, which induces Th2 cells to secrete type 2 cytokines such as IL-5 and IL-13. It plays a key role in various physiological and pathological processes including mucosal immunity, inflammation, and allergy. Accurate quantification of IL-33 is essential for elucidating its biological functions and evaluating the effects of pharmacological interventions.

Note: The above information is subject to change due to product optimization and updates. Please contact Ya Keyin Company for specific details.