IL-8 ELISA KIT 白介素-8ELISA试剂盒(人

产品概述

EliKine™ 人白介素-8 (IL-8) ELISA试剂盒 (货号: KTE6018-2) 是一款基于夹心法ELISA原理开发的高品质核心产品，专门用于定量检测人细胞培养上清、血清、血浆及其他生物液体样本中的IL-8含量。本试剂盒凭借其卓越的检测性能与可靠性，为细胞因子研究、免疫反应监测及疾病机制探索提供了强有力的工具。

核心特性与优势

• 高灵敏度与宽检测范围：最低检测浓度达2 pg/mL，线性检测范围为3.9 pg/mL - 250 pg/mL，确保对低丰度样本的精准定量。
• 卓越的特异性：经严格验证，与目标蛋白类似物无明显交叉反应或背景干扰，结果准确可靠。
• 即用型预包装：试剂盒组分齐全，包括预包被微孔板、标准品、检测抗体及全套检测试剂，优化了实验流程，节省准备时间。
• 稳定的重复性：推荐使用复孔检测，确保实验数据的稳定性和可重复性。

产品背景信息

白细胞介素-8 (IL-8) 是一种重要的趋化因子，由巨噬细胞、上皮细胞等多种细胞分泌，在基因ID 3576 (UniProt: P10145) 编码下发挥关键生理功能。IL-8通过招募和激活中性粒细胞参与炎症调节，并在血管生成、支气管炎、结肠癌等病理过程中扮演重要角色。此外，其表达水平与精神分裂症治疗反应、肥胖及囊性纤维化等疾病密切相关。

试剂盒组分

• 人源 IL-8 抗体预包被微孔板
• 人源 IL-8蛋白标准品
• 人源 IL-8检测抗体
• EliKine™ Streptavidin-HRP
• 标准品稀释液、实验缓冲液、HRP底物、反应终止液、洗涤缓冲液、封板膜

技术参数

反应性	人	检测方法	夹心法ELISA
样本类型	细胞培养上清、血清、血浆、其他生物液体
灵敏度	2 pg/mL	检测范围	3.9-250 pg/mL
保存条件	未开封试剂盒2-8℃保存；启用后请参照说明书保存各组分
运输条件	冰袋运输（蓝冰）

规格与价格

• 48 T：价格 1598.0 元 | 货期：现货（次日发货）
• 96 T：价格 2558.0 元 | 货期：现货（次日发货）

注意事项

• 请勿混合使用不同批号或过期试剂。
• 使用前请室温平衡30分钟，并按标签说明储存。
• 实验过程中建议使用一次性吸头，避免交叉污染。
• 未使用的板条需立即密封并4℃保存。
• 推荐使用低速振荡器或定期手动混匀，以确保反应充分。

警告：本品仅供研究使用，不适用于人类或临床诊断。使用前请查阅相关专利或许可证要求，避免侵权风险。

别名参考：NAF; GCP1; LECT; LUCT; NAP1; CXCL8; 白介素-8 ELISA试剂盒; Human IL-8 ELISA Kit

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

EliKine™ Human Interleukin-8 (IL-8) ELISA Kit (Catalog No.: KTE6018-2) is a high‑quality core product developed based on the sandwich‑ELISA principle, specifically designed for quantitative detection of IL‑8 in human cell culture supernatants, serum, plasma, and other biological fluid samples. This kit, with its outstanding analytical performance and reliability, provides a powerful tool for cytokine research, immune response monitoring, and disease‑mechanism investigation.

Key Features and Advantages

• High Sensitivity and Wide Dynamic Range: Minimum detectable concentration of 2 pg/mL, linear range of 3.9 pg/mL – 250 pg/mL, ensuring accurate quantification of low‑abundance samples.
• Excellent Specificity: Rigorously validated with no significant cross‑reactivity or background interference from homologous proteins, delivering accurate and reliable results.
• Ready‑to‑Use Pre‑packaged Format: Complete kit components including pre‑coated microplates, standards, detection antibody, and the full set of reagents, streamlining workflow and saving preparation time.
• Robust Reproducibility: Duplicate well testing is recommended to ensure data stability and repeatability.

Product Background Information

Interleukin‑8 (IL‑8) is an important chemokine secreted by macrophages, epithelial cells and various other cell types, encoded by gene ID 3576 (UniProt: P10145) and playing critical physiological roles. IL‑8 mediates inflammation by recruiting and activating neutrophils and is also involved in angiogenesis, bronchitis, colorectal cancer, and other pathological processes. Moreover, its expression level is closely associated with therapeutic response in schizophrenia, obesity, and cystic fibrosis.

Kit Components

• Human IL‑8 antibody pre‑coated microplate
• Human IL‑8 protein standard
• Human IL‑8 detection antibody
• EliKine™ Streptavidin‑HRP
• Standard diluent, assay buffer, HRP substrate, stop solution, wash buffer, plate sealing film

Technical Specifications

Species Reactivity	Human	Detection Method	Sandwich ELISA
Sample Types	Cell culture supernatants, serum, plasma, other biological fluids
Sensitivity	2 pg/mL	Detection Range	3.9–250 pg/mL
Storage Conditions	Unopened kit stored at 2–8 °C; after opening, store individual components as indicated in the manual
Shipping Conditions	Transport on ice packs (blue ice)

Specifications and Pricing

• 48‑test: Price ¥1,598.0 | Availability: In stock (next‑day shipment)
• 96‑test: Price ¥2,558.0 | Availability: In stock (next‑day shipment)

Precautions

• Do not mix reagents from different lot numbers or expired reagents.
• Allow reagents to equilibrate to room temperature for 30 minutes before use, and store according to label instructions.
• Use disposable pipette tips during the assay to avoid cross‑contamination.
• Unutilized plate strips should be immediately sealed and stored at 4 °C.
• Recommend gentle shaking or periodic manual mixing to ensure complete reaction.

Warning: This product is for research use only and is not intended for human or clinical diagnostic applications. Please review relevant patents or licensing requirements before use to avoid infringement risk.

Synonyms/References: NAF; GCP1; LECT; LUCT; NAP1; CXCL8; Interleukin‑8 ELISA Kit; Human IL‑8 ELISA Kit

Note: The above information may change as the product is optimized or upgraded; please contact EliKine™ for the most current details.