TGF-β1 ELISA KIT 转化生长因子-β1ELIS

人转化生长因子-β1 (TGF-β1) ELISA试剂盒

货号：KTE6030-2 | 反应性：人 | 检测方法：夹心法ELISA

产品概述

本试剂盒采用双抗体夹心酶联免疫吸附法（ELISA），专用于定量检测人血清、血浆、细胞培养上清及其他生物液体样本中的转化生长因子-β1（TGF-β1）蛋白浓度。该试剂盒是细胞分析领域的核心产品，为免疫学研究、疾病机制探索及生物标志物分析提供可靠工具。

核心特点与优势

• 高灵敏度：最低检测浓度可达8 pg/mL，适用于低丰度样本的精准定量。
• 优异特异性：经严格验证，与目标蛋白类似物无显著交叉反应或背景干扰。
• 宽线性范围：15.63 pg/mL - 1000 pg/mL的检测范围，满足多样本浓度需求。
• 即用型组分：预包被微孔板及优化试剂，减少操作步骤，提升实验效率。

试剂盒组分（48T/96T）

• 人TGF-β1抗体预包被微孔板
• 人TGF-β1蛋白标准品
• 人TGF-β1检测抗体
• EliKine™ Streptavidin-HRP
• 标准品稀释液、实验缓冲液、HRP底物、终止液
• 洗涤缓冲液、封板膜

样本类型

适用于细胞培养上清、血清、血浆及其他生物液体样本。

技术参数

检测范围	15.63 - 1000 pg/mL
灵敏度	8 pg/mL
种属反应性	人
基因ID	7040
蛋白质ID	P01137（UniProt链接：P01137）

背景信息

转化生长因子-β1（TGF-β1）是一种25 kDa的二聚体细胞因子，具有细胞生长抑制与促进双重功能，在组织修复、免疫调节及疾病进程中发挥关键作用。其基因高度保守，人与小鼠同源性达99%，是伤口愈合、骨软骨修复及自身免疫疾病研究的重要靶点。

注意事项

• 请勿混合不同批号或过期试剂使用。
• 使用前需室温平衡30分钟，建议样本与标准品进行复孔检测。
• 未使用的板条应密封后4℃保存，避免反复冻融。
• 实验过程中需使用一次性吸头，防止交叉污染。

保存与运输

保存：未开封试剂盒2-8℃保存；启用后请按说明书分装储存各组分。
运输：蓝冰冰袋运输，保障试剂活性。

规格与价格

• 48T：1598.0元（现货，次日发货）
• 96T：2558.0元（现货，次日发货）

警告：本品仅供科研使用，不适用于临床诊断。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human Transforming Growth Factor‑β1 (TGF‑β1) ELISA Kit

Catalog No.: KTE6030-2 | Species Reactivity: Human | Detection Method: Sandwich ELISA

Product Overview

This kit utilizes a double‑antibody sandwich enzyme‑linked immunosorbent assay (ELISA) for the quantitative detection of transforming growth factor‑β1 (TGF‑β1) protein levels in human serum, plasma, cell culture supernatants, and other biological fluid samples. It is a core product in cellular analysis, providing a reliable tool for immunological research, disease‑mechanism investigation, and biomarker analysis.

Key Features & Advantages

• High Sensitivity: Minimum detectable concentration of 8 pg/mL, suitable for precise quantification of low‑abundance samples.
• Excellent Specificity: Rigorously validated with no significant cross‑reactivity or background interference from homologous proteins.
• Broad Linear Range: 15.63 pg/mL – 1000 pg/mL, accommodating a wide range of sample concentrations.
• Ready‑to‑Use Components: Pre‑coated microplates and optimized reagents reduce procedural steps and increase workflow efficiency.

Kit Components (48T/96T)

• Human TGF‑β1 antibody‑coated microplate
• Human TGF‑β1 recombinant protein standard
• Human TGF‑β1 detection antibody
• EliKine™ Streptavidin‑HRP
• Standard dilution buffer, assay buffer, HRP substrate, stop solution
• Wash buffer, plate sealing film

Sample Types

Compatible with cell culture supernatants, serum, plasma, and other biological fluid samples.

Technical Specifications

Detection Range	15.63 – 1000 pg/mL
Sensitivity	8 pg/mL
Species Reactivity	Human
Gene ID	7040
Protein ID	P01137 (UniProt link: P01137)

Background

Transforming growth factor‑β1 (TGF‑β1) is a 25 kDa dimeric cytokine with dual functions of growth inhibition and promotion. It plays critical roles in tissue repair, immune regulation, and disease progression. The gene is highly conserved, showing 99% homology between human and mouse, making it a key target for studies of wound healing, osteochondral regeneration, and autoimmune diseases.

Precautions

• Do not mix reagents from different lot numbers or use expired components.
• Allow the kit to equilibrate to room temperature for 30 minutes before use; duplicate wells are recommended for samples and standards.
• Unused plates should be sealed and stored at 4 °C to avoid repeated freeze‑thaw cycles.
• Use disposable pipette tips throughout the assay to prevent cross‑contamination.

Storage & Shipping

Storage: Unopened kits should be stored at 2‑8 °C; after opening, aliquot and store each component according to the instruction manual.
Shipping: Transport on dry ice packs to preserve reagent activity.

Specifications & Pricing

• 48T: ¥1,598.0 (in stock, next‑day dispatch)
• 96T: ¥2,558.0 (in stock, next‑day dispatch)

Warning: This product is for research use only and is not intended for clinical diagnosis.

Note: The above information may be subject to change due to product optimization or upgrades. Please contact Yakebi Co. for the most current details.