人糖抗原125 ELISA定量试剂盒(Cancer Anti

Human Carbohydrate Antigen 125 (CA125) ELISA Kit (One Step Method)
人糖抗原125 ELISA定量试剂盒（单步法）

产品概览

货号：KTE4054-1   检测类型：ELISA（双抗体夹心法）
适用反应物种：人   样本类型：血清、血浆、组织、细胞上清及其他相关液体样本

核心应用

• 蛋白类分析——CA125（Cancer Antigen 125）定量检测
• ELISA平台的研究型实验
• 肿瘤标志物（尤其是卵巢癌相关）探索与机制研究

试剂盒组成

• Human CA125 Microplate（96孔或48孔预包覆板）
• Human CA125 Standard (200 U/mL)
• Standard Diluent
• Human CA125 Detect Antibody
• Streptavidin‑HRP
• HRP Substrate A & B（TMB显色底物）
• Stop Solution（酸性停止液）
• Wash Buffer
• Plate Covers（密封盖）

检测范围与灵敏度

• 检测范围：1.25 U/mL – 100 U/mL
• 检测灵敏度（LOD）：0.25 U/mL

技术优势

• 采用EliKine™技术，提供高灵敏度和高特异性的定量结果。
• 对CA125同系物的交叉反应极低，背景信号干扰可忽略。
• 一次加样，操作流程简洁，适合中高通量筛选。

检测原理（双抗体夹心法）

1. 微孔板预包被纯化的人CA125抗体，形成固相捕获层。
2. 加入标本或标准品，CA125抗原与捕获抗体特异性结合。
3. 加入生物素化的检测抗体，形成“抗体‑抗原‑抗体”复合物。
4. 加入链霉亲和素‑HRP，完成“抗体‑抗原‑抗体‑酶”三步组装。
5. 洗涤除去未结合成分后，加入TMB底物显色，HRP催化产生蓝色。
6. 加入Stop Solution，颜色转为黄色，用酶标仪在450 nm读取OD值，依据标准曲线计算样本中CA125浓度。

实验操作要点

• 所有试剂均应在使用前于室温（20‑25 °C）平衡30 分钟。
• 实验中请使用一次性吸头，防止交叉污染。
• 未使用的酶标板应立即放回原包装，密闭后在4 °C保存。
• 充分混匀（推荐低速振荡或每10 分钟手动轻摇）是确保反应均一的关键。
• 建议对每个样品和标准做双孔或三孔复测，以提升数据可靠性。

储存与运输

• 未开封的试剂盒请避光保存于2 °C – 8 °C，保质期6个月。
• 启用后的各组分请参照说明书中对应的保存条件进行管理。
• 运输方式：蓝色冰袋冷链运输，确保全程低温。

警示声明

本产品仅限于**科研用途**，不适用于临床诊断或人类治疗。任何因使用本产品而产生的专利侵权或其他法律责任，均由使用者自行承担。

订购规格及价格

规格	单价 (¥)	交货期
48 T（48孔/套）	1998.0	期货（3 天内发货）
96 T（96孔/套）	3198.0	期货（3 天内发货）

关键词检索

Cancer Antigen 125, Ovarian Cancer‑Related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human Carbohydrate Antigen 125 (CA125) ELISA Kit (One Step Method)
Human Carbohydrate Antigen 125 ELISA Quantitative Kit (One Step Method)

Product Overview

Catalog Number: KTE4054-1   Assay Type: ELISA (Sandwich Method)
Reactive Species: Human   Sample Types: Serum, plasma, tissue, cell culture supernatant, and other related liquid samples

Core Applications

• Protein Analysis – Quantitative detection of CA125 (Cancer Antigen 125)
• Research experiments on the ELISA platform
• Exploration and mechanism research of tumor markers (especially ovarian cancer-related)

Kit Components

• Human CA125 Microplate (96-well or 48-well pre-coated plate)
• Human CA125 Standard (200 U/mL)
• Standard Diluent
• Human CA125 Detect Antibody
• Streptavidin‑HRP
• HRP Substrate A & B (TMB Substrate)
• Stop Solution (Acidic Stop Solution)
• Wash Buffer
• Plate Covers

Detection Range & Sensitivity

• Detection Range: 1.25 U/mL – 100 U/mL
• Detection Sensitivity (LOD): 0.25 U/mL

Technical Advantages

• Utilizes EliKine™ technology, providing high sensitivity and high specificity quantitative results.
• Extremely low cross-reactivity with CA125 homologs; background signal interference is negligible.
• One-step addition; simple operation procedure, suitable for medium to high-throughput screening.

Assay Principle (Sandwich Method)

1. The microplate is pre-coated with purified human CA125 antibody, forming a solid-phase capture layer.
2. Specimens or standards are added; CA125 antigen specifically binds to the capture antibody.
3. Biotinylated detection antibody is added, forming an "antibody-antigen-antibody" complex.
4. Streptavidin-HRP is added, completing the three-step assembly of "antibody-antigen-antibody-enzyme".
5. After washing away unbound components, TMB substrate is added for color development; HRP catalysis produces a blue color.
6. Stop Solution is added, turning the color yellow. The OD value is read at 450 nm using a microplate reader, and the CA125 concentration in the sample is calculated based on the standard curve.

Key Experimental Operation Points

• All reagents should be equilibrated to room temperature (20‑25 °C) for 30 minutes before use.
• Use disposable pipette tips during the experiment to prevent cross-contamination.
• Unused microplate strips should be immediately returned to the original packaging, sealed, and stored at 4 °C.
• Thorough mixing (recommended low-speed shaking or manual gentle shaking every 10 minutes) is key to ensuring uniform reaction.
• It is recommended to perform duplicate or triplicate measurements for each sample and standard to improve data reliability.

Storage & Shipping

• The unopened kit should be stored protected from light at 2 °C – 8 °C; shelf life is 6 months.
• After opening, please manage each component according to the corresponding storage conditions in the instructions.
• Shipping method: Cold chain shipping with blue ice packs to ensure low temperature throughout the process.

Warning Statement

This product is intended for **research use only**, not for clinical diagnosis or human treatment. Any patent infringement or other legal liability arising from the use of this product shall be borne by the user.

Ordering Specifications & Price

Specification	Unit Price (¥)	Delivery Period
48 T (48 wells/kit)	1998.0	Future goods (ships within 3 days)
96 T (96 wells/kit)	3198.0	Future goods (ships within 3 days)

Keywords for Search

Cancer Antigen 125, Ovarian Cancer‑Related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125

Note: The above information may change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.