Thrombospondin-1/THBS-1 ELISA

产品概述

英文名称：Human Thrombospondin-1/THBS-1 ELISA Kit
中文名称：Thrombospondin-1/THBS-1 ELISA KIT 血小板反应素-1 ELISA 试剂盒（人）
货号：KTE3150-2
检测类型：ELISA（双抗体夹心法）
适用领域：细胞分析 → 蛋白类 → ELISA 应用（核心产品）

关键特性 & 优势

• 采用 EliKine™ 双抗体夹心技术，提供 极高的灵敏度（0.78 ng/mL） 与特异性。
• 检测范围宽广：0.78 ng/mL – 50 ng/mL，满足低至中等浓度样本的定量需求。
• 对 Thrombospondin-1（THBS-1）类似物无显著交叉反应，背景信号低。
• 配套完整的 48‑孔或 96‑孔规格，适配常规酶标仪（450 nm）读取。
• 严格的批次一致性控制，推荐使用同批次试剂完成整套实验。

检测原理

本套件基于双抗体夹心 ELISA 原理：

1. 微孔板预先包被特异性抗人 THBS-1 抗体（固相抗体）。
2. 加入样品或标准品，THBS-1 与固相抗体结合。
3. 加入生物素化的检测抗体，形成抗体‑抗原‑生物素三联体。
4. 加入链霉亲和素‑HRP 偶联物，实现生物素‑链霉亲和素特异性结合。
5. 添加 TMB 底物，在 HRP 催化下显色（蓝），随后加入终止液转为黄色。
6. 在 450 nm 波长读取 OD 值，OD 与 THBS-1 浓度呈正比关系。

适用样本

本套件经验证可直接用于以下样本类型：

• 细胞培养上清
• 血清
• 血浆

样本须预先离心去除残渣，并在 4 °C 保存至实验前。

试剂盒组成

• Human THBS-1 微孔板
• Human THBS-1 标准品（冻干）
• Assay Buffer（10×）
• Human THBS-1 检测抗体（100×）
• Streptavidin‑HRP（100×）
• HRP 底物（TMB）
• Stop Solution
• Wash Buffer（20×）
• Plate Covers（密封盖）

检测范围 & 灵敏度

• 检测范围：0.78 ng/mL – 50 ng/mL
• 灵敏度（检测下限）：0.78 ng/mL

使用注意事项

• 同一实验中请勿混用不同批号或超过有效期的试剂。
• 使用前请将所有试剂在室温平衡 30 分钟。
• 实验过程中采用一次性吸头，避免交叉污染。
• 未使用的板条请立即放回包装并密封，4 °C 保存。
• 每 10 分钟轻度振荡或手动摇匀，确保反应均一。
• 建议对每个样本或标准品做双孔或三孔复测，以提升数据可靠性。

保存与运输

未开封试剂盒：避光保存于 2‑8 °C，保质期 24 个月。
开启后：请参照说明书对各组分分别储存。

运输条件：使用蓝冰冷链（冰袋）进行保温运输。

规格、价格 & 交付

规格	单价（元）	交货周期
48 T	1,598.00	期货（5 天内发货）
96 T	2,558.00	期货（5 天内发货）

背景与应用

血小板反应素-1（Thrombospondin‑1, THBS‑1）是血小板反应素家族的重要成员，参与细胞‑细胞及细胞‑基质的相互作用。它可结合纤维蛋白原、纤维连接蛋白、层粘连蛋白、V 型胶原及整合素 α‑V/β‑1，在血小板聚集、血管生成以及肿瘤发生过程中发挥调控作用。THBS‑1 同时是新血管形成和肿瘤发展的天然抑制因子，能够正向或负向调节内皮细胞的黏附、迁移与增殖。通过阻断 THBS‑1 与 CD47 的相互作用，可增强肿瘤对放疗的免疫响应，促进肿瘤细胞死亡。

本 ELISA 试剂盒为科研提供可靠的定量手段，适用于：

• 血管生成和血小板功能相关的基础研究。
• 肿瘤微环境、细胞外基质互作及其信号通路的探索。
• 药物干预（如抗‑THBS‑1 抗体、CD47 阻断剂）效果的定量评估。

合规声明

本产品仅供科研使用，**不适用于人类或临床诊断**。使用本产品的任何专利或许可证问题，均由使用者自行负责，供应方不承担相应责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Human Thrombospondin-1/THBS-1 ELISA Kit
Chinese Name: Thrombospondin-1/THBS-1 ELISA KIT 血小板反应素-1 ELISA 试剂盒（人）
Catalog No.: KTE3150-2
Assay Type: ELISA (dual‑antibody sandwich)
Application Area: Cell Analysis → Protein Assays → ELISA Applications (core product)

Key Features & Advantages

• Utilizes EliKine™ dual‑antibody sandwich technology, delivering exceptionally high sensitivity (0.78 ng/mL) and specificity.
• Broad quantitative range: 0.78 ng/mL – 50 ng/mL, meeting low‑ to medium‑concentration sample needs.
• No significant cross‑reactivity with Thrombospondin‑1 (THBS‑1) homologues; low background signal.
• Complete 48‑well or 96‑well formats, compatible with standard microplate readers (450 nm).
• Stringent batch‑to‑batch consistency control; recommended to use reagents from the same lot for the entire experiment.

Assay Principle

This kit is based on the dual‑antibody sandwich ELISA principle:

1. Microplate wells are pre‑coated with a capture antibody specific for human THBS‑1 (solid‑phase antibody).
2. Samples or standards are added; THBS‑1 binds to the capture antibody.
3. A biotin‑labeled detection antibody is introduced, forming an antibody‑antigen‑biotin ternary complex.
4. Streptavidin‑HRP conjugate is added, achieving specific biotin‑streptavidin interaction.
5. TMB substrate is added; HRP catalyzes color development (blue), followed by stop solution turning the signal yellow.
6. Optical density is read at 450 nm; OD is directly proportional to THBS‑1 concentration.

Applicable Sample Types

The kit has been validated for direct use with the following sample matrices:

• Cell culture supernatants
• Serum
• Plasma

Samples should be centrifuged to remove debris and stored at 4 °C until analysis.

Kit Contents

• Human THBS‑1 microplate
• Human THBS‑1 standard (lyophilized)
• Assay Buffer (10×)
• Human THBS‑1 detection antibody (100×)
• Streptavidin‑HRP (100×)
• HRP substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers (seal)

Detection Range & Sensitivity

• Detection Range: 0.78 ng/mL – 50 ng/mL
• Limit of Detection (LOD): 0.78 ng/mL

Important Use Notes

• Do not mix reagents from different lots or use expired components within the same experiment.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable pipette tips throughout the procedure to avoid cross‑contamination.
• Unutilized plate strips should be returned to their original packaging, sealed, and stored at 4 °C.
• Gently vortex or manually shake every 10 minutes to ensure uniform reactions.
• Perform each sample or standard in duplicate or triplicate to improve data reliability.

Storage & Shipping

Unopened kit: Store protected from light at 2‑8 °C; shelf life 24 months.
After opening: Follow the user manual for individual component storage conditions.

Shipping conditions: Transport with ice packs under cooled (blue‑ice) conditions.

Specifications, Pricing & Delivery

Specification	Unit Price (CNY)	Delivery Lead Time
48 T	1,598.00	Spot‑market (dispatch within 5 days)
96 T	2,558.00	Spot‑market (dispatch within 5 days)

Background & Applications

Thrombospondin‑1 (THBS‑1) is a key member of the thrombospondin family, mediating cell‑cell and cell‑matrix interactions. It binds fibrinogen, fibronectin, laminin, type V collagen, and integrin α‑V/β‑1, influencing platelet aggregation, angiogenesis, and tumorigenesis. THBS‑1 acts as a natural inhibitor of neovascularization and tumor progression, modulating endothelial cell adhesion, migration, and proliferation positively or negatively. Blocking the THBS‑1/CD47 interaction can enhance the immune response to radiotherapy, promoting tumor cell death.

This ELISA kit provides a reliable quantitative tool for research, suitable for:

• Fundamental studies on angiogenesis and platelet function.
• Investigation of tumor microenvironment, extracellular matrix interactions, and associated signaling pathways.
• Quantitative evaluation of therapeutic interventions (e.g., anti‑THBS‑1 antibodies, CD47 blockers).

Regulatory Statement

This product is intended for research use only and **is not for human or clinical diagnostic applications**. Any patent or licensing issues arising from the use of this product are the sole responsibility of the user; the supplier disclaims all related liability.

Note: Information is subject to change due to product upgrades or optimizations. For the most current details, please contact Yakein Co., Ltd.