IGHG3/HDC ELISA KIT 免疫球蛋白G3ELI

Human IgG3 ELISA Kit (One Step Method)
IGHG3/HDC ELISA KIT 免疫球蛋白G3 ELISA 试剂盒（人）

产品定位

• 应用领域：蛋白类分析 – 细胞分析中心 – ELISA 检测
• 检测类型：双抗体夹心法（One‑Step）ELISA
• 适用样本：血清、血浆、组织匀浆、细胞上清以及其他相关液体样本
• 目标分子：人免疫球蛋白 G3（IgG3）

核心优势

• 极高灵敏度：0.5 μg/mL 检测下限
• 宽广定量范围：2.5 μg/mL – 200 μg/mL
• 特异性强：未检测到显著交叉反应或背景干扰
• 一步法操作简化实验流程，降低人为误差
• 配套完整，包含所有必需试剂和一次性耗材

试剂盒组分

• Human IgG3 Microplate（预包被）
• Human IgG3 Standard (400 μg/mL)
• Standard Diluent
• Human IgG3 Detect Antibody（生物素化）
• Streptavidin‑HRP
• HRP Substrate A & B（TMB 体系）
• Stop Solution
• Wash Buffer
• Plate Covers（防尘）

实验流程概述（建议 96 孔板）

1. 温育板至室温（30 min），轻轻摇匀。
2. 加入 100 µL 样品或标准稀释液至对应孔。
3. 加入 100 µL 检测抗体，37 ℃孵育 60 min。
4. 加入 100 µL Streptavidin‑HRP，37 ℃孵育 30 min。
5. 用 Wash Buffer 进行 5 次冲洗，每次 200 µL。
6. 加入 100 µL HRP Substrate A/B，室温暗避光 10‑15 min。
7. 加入 50 µL Stop Solution，终止反应。
8. 使用酶标仪在 450 nm（参考 630 nm）读取 OD 值，绘制标准曲线。

建议每个样本设置双孔或三孔（复孔）以提高数据可靠性。

技术参数

检测范围	2.5 μg/mL – 200 μg/mL
灵敏度	0.5 μg/mL
样本体积	≈100 µL/孔（根据实验需求可调）
检测原理	双抗体夹心法 + HRP‑TMB 发色
适用物种	人（Human）
储存条件	未开封：2‑8 °C，避光；开封后请参照说明书分别保存各组分
有效期	6 个月（自生产日期起）
运输方式	冰袋（蓝冰）冷链运输

包装规格 & 价格

包装规格	单价（人民币）	交货周期
48 T	1998.0	期货（3 天内发货）
96 T	3198.0	期货（3 天内发货）

使用注意事项

• 不同批号或过期的试剂不得混用。
• 所有试剂使用前需在室温平衡 30 分钟。
• 实验操作应使用一次性吸头，防止交叉污染。
• 未使用的板条请立即放回原包装、密封保存于 4 °C。
• 混匀对结果影响显著，推荐低速振荡或每 10 分钟手动轻摇一次。
• 样品与标准建议进行双孔或三孔检测（复孔），以提升数据可重复性。

安全与合规声明

本试剂盒仅供科研用途，不适用于临床诊断或人类治疗。使用本产品的任何专利或许可证相关行为，风险与责任均由使用者自行承担。

产品背景

EliKine™ 人 IgG3 ELISA 定量试剂盒采用双抗体夹心法，将抗人 IgG3 的特异性捕获抗体预包被于酶标板孔内。样品中的 IgG3 与捕获抗体结合后，加入生物素化检测抗体形成“捕获‑抗原‑检测”复合物；随后加入链霉亲和素‑HRP，使酶标复合物得以显色。显色后在 450 nm 读取光密度，OD 与 IgG3 浓度呈正比，可通过标准曲线实现定量。

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关键词：IGHG3, HDC, Heavy chain disease protein, Ig Gamma‑3 Chain C Region, Immunoglobulin Heavy Constant Gamma 3, G3m Marker, Immunoglobulin Gm3

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human IgG3 ELISA Kit (One Step Method)
IGHG3/HDC ELISA Kit Immunoglobulin G3 ELISA Kit (Human)

Product Positioning

• Application field: Protein analysis – Cell analysis core – ELISA detection
• Assay type: Sandwich double‑antibody (One‑Step) ELISA
• Applicable samples: Serum, plasma, tissue homogenate, cell supernatant and other related liquid samples
• Target molecule: Human immunoglobulin G3 (IgG3)

Key Advantages

• Ultra‑high sensitivity: detection limit 0.5 μg/mL
• Broad quantitative range: 2.5 μg/mL – 200 μg/mL
• High specificity: no significant cross‑reactivity or background interference detected
• One‑step protocol simplifies workflow and reduces human error
• Complete kit includes all required reagents and disposables

Kit Components

• Human IgG3 Microplate (pre‑coated)
• Human IgG3 Standard (400 μg/mL)
• Standard Diluent
• Human IgG3 Detect Antibody (biotinylated)
• Streptavidin‑HRP
• HRP Substrate A & B (TMB system)
• Stop Solution
• Wash Buffer
• Plate Covers (dust‑proof)

Overview of Procedure (96‑well plate recommended)

1. Incubate plate to room temperature (≈30 min) and gently shake to equilibrate.
2. Add 100 µL of sample or standard dilution to the designated wells.
3. Add 100 µL detection antibody; incubate at 37 °C for 60 min.
4. Add 100 µL Streptavidin‑HRP; incubate at 37 °C for 30 min.
5. Wash 5 times with Wash Buffer, 200 µL per wash.
6. Add 100 µL HRP Substrate A/B; incubate in the dark at room temperature for 10–15 min.
7. Add 50 µL Stop Solution to terminate the reaction.
8. Measure optical density at 450 nm (reference 630 nm) using a microplate reader and generate the standard curve.

It is recommended to run each sample in duplicate or triplicate (replicate wells) to enhance data reliability.

Technical Specifications

Detection range	2.5 μg/mL – 200 μg/mL
Sensitivity	0.5 μg/mL
Sample volume	≈100 µL per well (adjustable per experimental need)
Assay principle	Sandwich double‑antibody + HRP‑TMB color development
Applicable species	Human
Storage conditions	Unopened: 2‑8 °C, protected from light; after opening, follow the manual for each component
Shelf life	6 months from manufacturing date
Shipping method	Ice pack (blue ice) cold‑chain transport

Packaging Specification & Price

Package Size	Unit Price (CNY)	Delivery Lead Time
48 T	1998.0	Spot (shipment within 3 days)
96 T	3198.0	Spot (shipment within 3 days)

Precautions

• Do not mix reagents from different batches or expired lots.
• All reagents must be brought to room temperature for 30 minutes before use.
• Use disposable pipette tips to prevent cross‑contamination.
• Unused plate strips should be returned to their original packaging and stored sealed at 4 °C promptly.
• Mixing significantly affects results; recommend low‑speed orbital shaking or manually invert gently every 10 minutes.
• Run samples and standards in duplicate or triplicate to improve reproducibility.

Safety and Compliance Statement

This kit is for research use only and is not intended for clinical diagnostics or human therapy. Any patent or license‑related activities associated with this product are undertaken at the user’s own risk and liability.

Product Background

The EliKine™ Human IgG3 ELISA Quantitative Kit utilizes a sandwich double‑antibody format, with a specific capture antibody against human IgG3 pre‑coated onto microplate wells. IgG3 in the sample binds to the capture antibody, followed by addition of a biotinylated detection antibody to form a “capture‑antigen‑detection” complex; subsequently, Streptavidin‑HRP is added to generate color. After development, optical density is read at 450 nm; OD is directly proportional to IgG3 concentration, allowing quantitation via the standard curve.

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Keywords: IGHG3, HDC, Heavy chain disease protein, Ig Gamma‑3 Chain C Region, Immunoglobulin Heavy Constant Gamma 3, G3m Marker, Immunoglobulin Gm3

Note: The above information may change due to product optimization or upgrades; please contact Yakein Co. for the latest details.