Apolipoprotein A1/Apo A1 ELISA

产品概述

EliKine™ 人载脂蛋白A1 (Apo A1) ELISA试剂盒是一款专门用于定量检测人血清、血浆及细胞培养上清液中Apo A1含量的核心试剂盒。该试剂盒采用成熟的夹心法ELISA原理，具有卓越的检测性能，为心血管疾病研究、脂质代谢分析及临床前研究提供了可靠的工具。

核心参数

• 产品货号：KTE3149-2
• 检测物种：人 (Human)
• 样本类型：细胞培养上清、血清、血浆
• 检测范围：0.63 ng/mL - 40 ng/mL
• 灵敏度：0.63 ng/mL
• 规格选择：
  • 48 T - 价格：1598.0元 (货期：请咨询)
  • 96 T - 价格：2558.0元 (货期：期货，5天内发货)

产品特点与优势

• 高灵敏度与特异性：基于EliKine™平台优化，对目标蛋白表现出极高的检测灵敏度和特异性。经严格验证，与类似物无明显交叉反应或背景干扰。
• 可靠的检测性能：宽泛的检测范围（0.63-40 ng/mL）使其能够精准定量不同浓度水平的样本。
• 操作便捷：试剂盒提供完整的预包装组分，优化后的实验流程减少了操作步骤，提高了实验效率的一致性。
• 批次稳定性：严格的质量控制确保不同批次间试剂盒性能的稳定可靠。

试剂盒组分

• Human Apo A1 预包被微孔板
• Human Apo A1 标准品 (冻干)
• Assay Buffer (10×浓缩)
• Human Apo A1 检测抗体 (100×浓缩)
• 链霉亲和素-HRP (100×浓缩)
• HRP底物 (TMB)
• 终止液
• 洗涤液 (20×浓缩)
• 板盖

背景介绍

载脂蛋白A1 (ApoA1)是高密度脂蛋白(HDL)的主要结构蛋白，在胆固醇逆向转运过程中发挥核心作用，负责将外周组织的胆固醇运至肝脏进行代谢。ApoA1水平及其与ApoB-100的比率是评估心血管疾病风险的重要生物标志物，与心肌梗死等疾病的预测密切相关。此外，ApoA1基因缺陷也与丹吉尔病等遗传性疾病相关。本试剂盒为深入研究ApoA1的生物学功能及相关疾病机制提供了精准的检测手段。

检测原理

本试剂盒采用双抗体夹心法。微孔板预包被有抗人Apo A1抗体，样本中的Apo A1与固相抗体结合后，依次加入生物素化的检测抗体和链霉亲和素-HRP偶联物，形成复合物。加入TMB底物显色，颜色深浅与样本中Apo A1浓度成正比，通过测定OD450值进行定量分析。

注意事项与保存

• 请勿混合不同批号或已过有效期的试剂。
• 使用前，所有试剂应在室温平衡30分钟。
• 实验过程中请使用一次性吸头，避免交叉污染。
• 建议对样品和标准品进行复孔检测，以确保结果的准确性。
• 保存建议：未开封的试剂盒请避光保存于2-8℃，保质期24个月。启用后，请参照说明书妥善保存各组分。
• 运输条件：冰袋（蓝冰）运输。

重要声明

本产品仅供科学研究使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定。对于因使用本产品可能导致的专利侵权或其他违规行为，我方不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

The EliKine™ Human Apolipoprotein A1 (Apo A1) ELISA Kit is a core kit specifically designed for the quantitative detection of Apo A1 levels in human serum, plasma, and cell culture supernatants. This kit employs the well-established sandwich ELISA principle and offers excellent detection performance, providing a reliable tool for cardiovascular disease research, lipid metabolism analysis, and preclinical studies.

Core Parameters

• Catalog Number: KTE3149-2
• Species Reactivity: Human
• Sample Types: Cell culture supernatants, Serum, Plasma
• Detection Range: 0.63 ng/mL - 40 ng/mL
• Sensitivity: 0.63 ng/mL
• Size Options:
  • 48 T - Price: 1598.0 CNY (Availability: Please inquire)
  • 96 T - Price: 2558.0 CNY (Availability: Stock, ships within 5 days)

Features and Advantages

• High Sensitivity and Specificity: Optimized based on the EliKine™ platform, it demonstrates extremely high detection sensitivity and specificity for the target protein. Rigorously validated, it shows no significant cross-reactivity or background interference with analogues.
• Reliable Detection Performance: The wide detection range (0.63-40 ng/mL) enables accurate quantification of samples with varying concentration levels.
• User-Friendly Operation: The kit provides complete pre-packaged components. The optimized protocol reduces operational steps and improves experimental efficiency and consistency.
• Lot-to-Lot Consistency: Strict quality control ensures stable and reliable performance across different kit lots.

Kit Components

• Human Apo A1 Pre-coated Microplate
• Human Apo A1 Standard (Lyophilized)
• Assay Buffer (10× Concentrate)
• Human Apo A1 Detection Antibody (100× Concentrate)
• Streptavidin-HRP (100× Concentrate)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20× Concentrate)
• Plate Sealer

Background

Apolipoprotein A1 (ApoA1) is the primary structural protein of High-Density Lipoprotein (HDL) and plays a central role in reverse cholesterol transport, responsible for transporting cholesterol from peripheral tissues to the liver for metabolism. ApoA1 levels and its ratio to ApoB-100 are important biomarkers for assessing cardiovascular disease risk and are closely related to the prediction of conditions such as myocardial infarction. Furthermore, ApoA1 gene defects are associated with inherited diseases like Tangier disease. This kit provides a precise detection method for in-depth research into the biological functions of ApoA1 and related disease mechanisms.

Assay Principle

This kit uses a double-antibody sandwich method. The microplate is pre-coated with an anti-human Apo A1 antibody. After Apo A1 in the sample binds to the solid-phase antibody, a biotinylated detection antibody and then a Streptavidin-HRP conjugate are added sequentially to form a complex. The addition of TMB substrate produces a colorimetric reaction, where the color intensity is proportional to the concentration of Apo A1 in the sample. Quantification is achieved by measuring the OD450 value.

Precautions and Storage

• Do not mix reagents from different lots or expired kits.
• All reagents should be equilibrated to room temperature for 30 minutes before use.
• Use disposable pipette tips during the experiment to avoid cross-contamination.
• It is recommended to perform duplicate tests for both samples and standards to ensure result accuracy.
• Storage Recommendation: Store the unopened kit protected from light at 2-8°C; it is stable for 24 months. After opening, store individual components properly as instructed in the manual.
• Shipping Conditions: Shipped with ice packs (blue ice).

Important Notice

This product is for research use only, not intended for human or clinical diagnostic use. Users should ensure their application does not infringe upon any patents or license agreements. We assume no responsibility for patent infringements or other violations that may result from the use of this product.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Yakorin Inc. for specific details.