fT3 ELISA KIT 游离三碘甲状腺原氨酸ELISA试

Free Triiodothyronine (fT3) ELISA Kit — 游离三碘甲状腺原氨酸 ELISA 试剂盒 (牛)

产品概述

本套装为 EliKine™ 系列核心产品，专为 ELISA（竞争法） 设计，可在血清、血浆及其他生物液体中对 游离三碘甲状腺原氨酸（fT3） 进行定量检测。适用于牛以及包括人、小鼠、大鼠、山羊、绵羊、狗、兔子、猴子、鸡、猪等多种动物种属的研究样本。

关键应用

• 蛋白类与甲状腺功能相关的基础研究
• ELISA 免疫分析平台的细胞分析与激素水平监测
• 跨种属的比较性甲状腺功能研究

检测原理

本套件采用 竞争酶联免疫吸附法（Competition ELISA）：样本中的 fT3 与酶标记的 fT3（生物素偶联）竞争结合预包被在微孔板上的 fT3 抗体。随后加入 Avidin‑HRP 复合物并通过 HRP 底物显色，光密度（OD）与样本中 fT3 含量呈负相关，通过标准曲线进行定量。

检测范围与灵敏度

• 检测范围：2 pmol/L – 32 pmol/L
• 灵敏度（检测下限）：2.0 pmol/L

产品组成

• fT3 抗体预包被微孔板
• fT3 标准品（梯度浓度）
• 生物素偶联的 fT3
• Avidin‑HRP 复合物
• HRP 底物 A & B（显色剂）
• 反应终止液
• 洗涤缓冲液
• 封板膜（防尘）

使用注意事项

• 不同批号或已过期的试剂不得混用。
• 使用前请将所有试剂在室温下平衡 30 分钟。
• 实验过程中请使用一次性吸头，防止交叉污染。
• 未使用的板条需立即放回原包装，密闭保存于 4 ℃。
• 充分混匀是获得可靠结果的前提，推荐使用低速振荡器或每 10 分钟手动轻摇一次。
• 建议对每个样品及标准品进行双孔或三孔复测，以提高数据的重复性。

存储与运输

• 未开封试剂盒请存于 2 °C–8 °C（冷藏）。
• 启用后请依据说明书对各组分进行相应温度或光暗条件的保存。
• 运输方式：冰袋（蓝冰）冷链运输，确保全程温度控制。

安全与合规

警告：本产品仅供科研使用，严禁用于人类临床诊断或治疗。使用本套件不构成对任何专利或许可证的侵权行为的授权，若因使用产生的专利纠纷或其他违规行为，本公司不承担责任。

技术规格 & 采购信息

规格	包装数量	单价 (CNY)	交货周期
48 T	48 tests	1598.0	期货（4 天内发货）
96 T	96 tests	2558.0	期货（4 天内发货）

产品编号 & 参考信息

• 货号：KTE0004‑B7‑2
• 检测指标：fT3（游离三碘甲状腺原氨酸）
• 适用物种：人、鼠、猪、狗、牛等多种动物
• 别名检索词：游离三碘甲状腺原氨酸 ELISA试剂盒; fT3; fT3 ELISA试剂盒; KET0004

背景信息（供参考）

血清中的总甲状腺素（T4）和总三碘甲状腺原氨酸（T3）大部分（>99%）与结合蛋白（主要是甲状腺素结合球蛋白，TBG）结合。游离形式的 T4（fT4）和 fT3 才是具有生物活性的部分，能够真实反映甲状腺功能状态，而不受蛋白结合的影响。因此，精准测定血清或血浆中的 fT3 对甲状腺研究尤为关键。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Free Triiodothyronine (fT3) ELISA Kit — Free Triiodothyronine ELISA Kit (Bovine)

Product Overview

This kit is a core product of the EliKine™ series, specifically designed for ELISA (Competitive Method) to quantitatively detect Free Triiodothyronine (fT3) in serum, plasma, and other biological fluids. It is suitable for research samples from bovine and various other animal species, including human, mouse, rat, goat, sheep, dog, rabbit, monkey, chicken, and pig.

Key Applications

• Basic research on proteins and thyroid function
• Cellular analysis and hormone level monitoring on the ELISA immunoassay platform
• Comparative thyroid function studies across species

Assay Principle

This kit employs the Competitive Enzyme-Linked Immunosorbent Assay (Competition ELISA): fT3 in the sample competes with enzyme-labeled fT3 (biotin-conjugated) for binding to the fT3 antibody pre-coated on the microplate. Subsequently, the Avidin-HRP complex is added, and color is developed via HRP substrate. The optical density (OD) is inversely proportional to the fT3 concentration in the sample, allowing quantification via a standard curve.

Detection Range & Sensitivity

• Detection Range: 2 pmol/L – 32 pmol/L
• Sensitivity (Lower Detection Limit): 2.0 pmol/L

Kit Components

• fT3 Antibody Pre-coated Microplate
• fT3 Standards (Gradient Concentrations)
• Biotin-conjugated fT3
• Avidin-HRP Conjugate
• HRP Substrate A & B (Chromogen)
• Stop Solution
• Wash Buffer
• Plate Sealer (Dust Cover)

Usage Notes

• Do not mix reagents from different lots or expired kits.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable pipette tips throughout the procedure to prevent cross-contamination.
• Unused strips should be immediately returned to the original packaging and sealed for storage at 4 °C.
• Thorough mixing is essential for reliable results; use a low-speed shaker or manually shake gently every 10 minutes.
• It is recommended to perform duplicate or triplicate measurements for each sample and standard to improve data reproducibility.

Storage & Shipping

• Store unopened kit at 2 °C–8 °C (refrigeration).
• After opening, store components according to the instructions at specified temperatures or under light/dark conditions.
• Shipping Method: Shipped with ice packs (blue ice) under cold chain conditions to ensure temperature control throughout transit.

Safety & Compliance

Warning: This product is for research use only. Not for use in human clinical diagnosis or treatment. The use of this kit does not grant any license or authorization under any patent rights. The company assumes no responsibility for patent disputes or other violations arising from the use of this product.

Specifications & Ordering Information

Specification	Package Size	Unit Price (CNY)	Delivery Lead Time
48 T	48 tests	1598.0	Future supply (ships within 4 days)
96 T	96 tests	2558.0	Future supply (ships within 4 days)

Product Number & Reference Information

• Catalog Number: KTE0004‑B7‑2
• Analyte: fT3 (Free Triiodothyronine)
• Applicable Species: Human, mouse, pig, dog, bovine, and other animals
• Alias/Search Terms: Free Triiodothyronine ELISA Kit; fT3; fT3 ELISA Kit; KET0004

Background Information (For Reference)

The majority (>99%) of total thyroxine (T4) and total triiodothyronine (T3) in serum are bound to binding proteins (primarily thyroxine-binding globulin, TBG). The free forms of T4 (fT4) and fT3 are the biologically active fractions, which accurately reflect thyroid functional status without being influenced by protein binding. Therefore, accurate measurement of fT3 in serum or plasma is particularly critical for thyroid research.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Yacoin Company for specific details.