T4 ELISA KIT 甲状腺素ELISA试剂盒(牛)

EliKine™ 牛甲状腺素 (T4) ELISA 检测试剂盒

货号：KTE0007-B7-2

产品类型：竞争法ELISA核心试剂盒

应用中心：细胞分析

产品概述

本试剂盒采用高效的竞争法ELISA原理，专为精准定量检测牛血清、血浆及其他生物液体样本中的甲状腺素 (T4) 含量而设计。作为甲状腺功能评估的基础筛选工具，该试剂盒在药物研发、兽医诊断及内分泌研究领域具有重要的应用价值。

核心特性与优势

• 广泛的物种反应性：经验证，适用于人、小鼠、大鼠、山羊、绵羊、狗、兔子、牛、猴子、鸡、猪等多种物种的样本检测。
• 高灵敏度与特异性：检测灵敏度达 10 ng/ml，检测范围为 20 ng/ml - 320 ng/ml。经严格测试，对待测物类似物未发现明显的交叉反应或背景干扰，确保结果的可靠性。
• 预包被微孔板：提供T4抗体预包被板，简化操作步骤，节省实验准备时间，有利于实验的标准化和高通量筛选。
• 优化的试剂系统：包含生物素-Avidin-HRP信号放大系统，有效提升检测信号，使结果更加稳定和清晰。

检测参数

检测方法	竞争法ELISA
样本类型	血清、血浆、其它生物液体
检测范围	20 ng/ml - 320 ng/ml
灵敏度	10 ng/ml

试剂盒组分

• T4 抗体预包被微孔板
• T4 标准品
• 生物素偶联的T4
• Avidin-HRP
• HRP底物A & B
• 反应终止液
• 洗涤缓冲液
• 封板膜

操作注意事项

• 请勿混合使用不同批号或超出有效期的试剂。
• 使用前所有试剂需室温平衡30分钟。
• 为避免交叉污染，实验过程中请使用一次性吸头。
• 未使用的板条应立即放回包装袋并密封，于4℃保存。
• 充分混匀是确保结果准确的关键，建议使用低速振荡器或定期手动摇匀。
• 为获得更可靠的数据，建议对样品和标准品进行复孔（双孔或三孔）检测。

保存与运输

保存建议：未开封的试剂盒请保存于2-8℃。启用后，请参照说明书妥善保存各组分。

运输条件：冰袋（蓝冰）运输，以确保试剂活性。

规格与价格

规格	价格 (人民币)	货期
48 T	1,598.0	期货（预计4个工作日内发货）
96 T	2,558.0	期货（预计4个工作日内发货）

重要提示：本产品仅供研究使用，不适用于人类或临床诊断。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Bovine Thyroxine (T4) ELISA Detection Kit

Catalog No.: KTE0007-B7-2

Product Type: Competitive ELISA Core Kit

Application Area: Cellular Analysis

Product Overview

This kit utilizes a highly efficient competitive ELISA format, specifically engineered for precise quantitative measurement of thyroxine (T4) concentrations in bovine serum, plasma, and other biological fluid samples. As a foundational screening tool for thyroid function assessment, the kit holds significant utility in drug development, veterinary diagnostics, and endocrine research.

Key Features and Advantages

• Broad Species Reactivity: Validated for sample detection across multiple species including human, mouse, rat, goat, sheep, dog, rabbit, bovine, monkey, chicken, and pig.
• High Sensitivity and Specificity: Detection sensitivity reaches 10 ng/mL with a quantifiable range of 20 ng/mL – 320 ng/mL. Rigorous testing revealed no significant cross‑reactivity or background interference from structurally related analytes, ensuring result reliability.
• Pre‑coated Microplates: T4 antibody‑coated plates are supplied, simplifying workflow, reducing assay preparation time, and facilitating standardized high‑throughput screening.
• Optimized Reagent System: Includes a biotin‑Avidin‑HRP signal amplification module, effectively enhancing detection signals for more stable and clear outcomes.

Assay Parameters

Assay Method	Competitive ELISA
Sample Type	Serum, plasma, other biological fluids
Dynamic Range	20 ng/mL - 320 ng/mL
Sensitivity	10 ng/mL

Kit Components

• T4 Antibody Pre‑coated Microplate
• T4 Standard
• Biotin‑conjugated T4
• Avidin‑HRP
• HRP Substrate A & B
• Reaction Stop Solution
• Wash Buffer
• Plate Sealing Film

Operational Considerations

• Do not mix reagents from different lots or beyond their expiration dates.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable tips throughout the assay to avoid cross‑contamination.
• Unutilized plate strips should be promptly returned to their packaging, sealed, and stored at 4 °C.
• Thorough mixing is critical for accurate results; a low‑speed shaker or periodic manual inversion is recommended.
• For increased data reliability, perform replicates (duplicate or triplicate wells) for samples and standards.

Storage and Shipping

Storage Recommendation: Store unopened kits at 2–8 °C. After opening, follow the instruction manual for proper storage of individual components.

Shipping Conditions: Ship on ice packs (blue ice) to maintain reagent activity.

Specifications and Pricing

Specification	Price (CNY)	Lead Time
48 T	1,598.0	In stock (estimated shipment within 4 business days)
96 T	2,558.0	In stock (estimated shipment within 4 business days)

Important Note: This product is for research use only and is not intended for human or clinical diagnostic applications.

Note: The above information may change due to product optimization or upgrades. For the most current details, please contact Yakein Inc.