人脂质运载蛋白2 ELISA定量试剂盒(Lipocalin-

Human Lipocalin-2/NGAL ELISA Kit (人脂质运载蛋白2 ELISA定量试剂盒)

货号: KTE3005-1

产品类型: EliKine™ 夹心法ELISA试剂盒

产品分类: 核心产品 - 细胞分析

规格与价格:

• 48 T: 1598.0元 (期货，预计5个工作日内发货)
• 96 T: 2558.0元 (货期请咨询)

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产品概述

本试剂盒是一款基于双抗体夹心法原理的高质量ELISA检测试剂盒，专门用于精准定量人源样本（如细胞培养上清、血清、血浆）中的脂质运载蛋白2 (Lipocalin-2/NGAL)含量。该试剂盒是研究肾脏损伤、先天免疫及相关疾病机制的理想工具。

背景信息

脂质运载蛋白2 (Lipocalin-2)，也被称为中性粒细胞明胶酶相关脂质运载蛋白（NGAL），是一种重要的功能性蛋白。它主要由中性粒细胞表达，并在肾脏、前列腺及呼吸道等组织的上皮细胞中低水平表达。Lipocalin-2通过螯合铁参与先天免疫反应，从而有效限制细菌生长。更重要的是，它已被广泛认为是急性肾损伤（AKI）的早期、灵敏生物标志物，在损伤发生后2小时内即可在血液和尿液中检测到其水平显著升高，且其浓度与损伤严重程度呈正相关，在慢性肾病、造影剂肾病等诊断中也具有重要价值。

检测原理

本试剂盒采用经典的双抗体夹心法。预先包被在微孔板上的特异性抗体捕获样本中的Lipocalin-2，随后通过生物素化的检测抗体、链霉亲和素-HRP偶联物以及TMB底物进行信号放大和检测。最终在450 nm波长下测定吸光度（OD值），其信号强度与样本中Lipocalin-2的浓度成正比。

核心特点与优势

• 高灵敏度：最低检测浓度可达125 pg/mL。
• 宽检测范围：提供125 pg/mL 至 8,000 pg/mL的宽广定量范围，适用于不同浓度的样本检测。
• 优异特异性：经严格验证，对目标蛋白的类似物无明显交叉反应或背景干扰，确保结果准确可靠。
• 操作便捷：试剂盒提供完整组分，优化后的实验流程方便快捷。

试剂盒组分

• 预包被Human Lipocalin-2微孔板
• Human Lipocalin-2标准品（冻干）
• 10× Assay Buffer
• 100× Human Lipocalin-2检测抗体（生物素标记）
• 100× Streptavidin-HRP
• HRP显色底物 (TMB)
• 终止液
• 20× 洗涤液
• 板盖

样本类型

适用于检测细胞培养上清、血清、血浆中的人Lipocalin-2。

保存与运输

保存：未开封试剂盒请避光保存于2-8℃，保质期24个月。启用后，请参照说明书妥善保存各组分。

运输：全程蓝冰（冰袋）低温运输，保障试剂活性。

重要注意事项

• 请勿混合使用不同批号或已过有效期的试剂。
• 使用前所有试剂应室温平衡约30分钟。
• 为避免交叉污染，实验过程中请使用一次性吸头。
• 未使用的板条需立即放回原包装袋并密封，置于4℃保存。
• 实验过程中充分的混匀至关重要，建议使用低速振荡器或定期手动摇匀。
• 为获得更准确的结果，建议对样品和标准品进行复孔（双孔或三孔）检测。

警告：本产品仅用于科学研究用途，不适用于人类或临床诊断。使用前请务必查阅产品说明书。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human Lipocalin-2/NGAL ELISA Kit

Catalog Number: KTE3005-1

Product Type: EliKine™ Sandwich ELISA Kit

Product Category: Core Products - Cell Analysis

Specifications & Price:

• 48 T: ¥1598.0 (Futures, expected to ship within 5 business days)
• 96 T: ¥2558.0 (Please inquire for availability)

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Product Overview

This kit is a high-quality ELISA detection kit based on the sandwich ELISA principle, specifically designed for the accurate quantification of Human Lipocalin-2/NGAL in human samples (such as cell culture supernatant, serum, plasma). It is an ideal tool for researching kidney injury, innate immunity, and related disease mechanisms.

Background Information

Lipocalin-2 (LCN2), also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), is an important functional protein. It is primarily expressed by neutrophils and is expressed at low levels in epithelial cells of tissues such as the kidney, prostate, and respiratory tract. Lipocalin-2 participates in innate immune responses by sequestering iron, thereby effectively limiting bacterial growth. More importantly, it has been widely recognized as an early, sensitive biomarker for Acute Kidney Injury (AKI), with significantly elevated levels detectable in blood and urine within 2 hours of injury onset. Its concentration is positively correlated with the severity of injury and also holds significant value in the diagnosis of conditions such as chronic kidney disease and contrast-induced nephropathy.

Assay Principle

This kit employs the classic sandwich ELISA method. A specific antibody pre-coated on the microplate captures Lipocalin-2 from the sample. Signal amplification and detection are then achieved using a biotinylated detection antibody, streptavidin-HRP conjugate, and TMB substrate. The final absorbance (OD value) is measured at 450 nm, and the signal intensity is proportional to the concentration of Lipocalin-2 in the sample.

Key Features & Advantages

• High Sensitivity: Minimum detectable concentration can reach 125 pg/mL.
• Wide Detection Range: Provides a broad quantification range from 125 pg/mL to 8,000 pg/mL, suitable for samples with varying concentrations.
• Excellent Specificity: Rigorously validated to show no significant cross-reactivity or background interference
• User-Friendly Operation: The kit provides complete components, and the optimized protocol is convenient and efficient.

Kit Components

• Pre-coated Human Lipocalin-2 Microplate
• Human Lipocalin-2 Standard (Lyophilized)
• 10× Assay Buffer
• 100× Human Lipocalin-2 Detection Antibody (Biotin-labeled)
• 100× Streptavidin-HRP
• HRP Colorimetric Substrate (TMB)
• Stop Solution
• 20× Wash Buffer
• Plate Sealers

Sample Types

Suitable for detecting Human Lipocalin-2 in cell culture supernatant, serum, and plasma.

Storage & Shipping

Storage: Store the unopened kit protected from light at 2-8°C. Shelf life is 24 months. After opening, please store the components properly according to the instructions.

Shipping: Shipped with blue ice (ice packs) to maintain a low temperature throughout transit, ensuring reagent activity.

Important Notes

• Do not mix reagents from different lots or use reagents beyond their expiration date.
• All reagents should be brought to room temperature and equilibrated for approximately 30 minutes before use.
• To avoid cross-contamination, use disposable pipette tips throughout the assay procedure.
• Unused strips should be returned to the original packaging bag immediately, sealed, and stored at 4°C.
• Thorough mixing is crucial during the assay; using a low-speed shaker or regular manual shaking is recommended.
• For more accurate results, it is recommended to test samples and standards in duplicate or triplicate.

Warning: This product is for research use only. Not for use in human or clinical diagnosis. Please refer to the product manual before use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Yakeyin Company for specific details.