T4 ELISA KIT 甲状腺素ELISA试剂盒(人)

产品概述

英文名称：Thyroxine (T4) ELISA Kit
中文名称：T4 ELISA KIT 甲状腺素 ELISA 试剂盒（人）
货号：KTE0007-2

适用领域

• 产品类别：核心产品 → 细胞分析 → ELISA
• 一级应用：ELISA（竞争法）
• 主要检测对象：血清总甲状腺素（T4）
• 检测类型：ELISA（竞争法）
• 样本类型：血清、血浆、其它生物液体

技术要点

检测范围	20 ng/ml – 320 ng/ml
灵敏度	10 ng/ml
交叉反应	未发现显著交叉反应或背景干扰
竞争试剂	生物素偶联的 T4
检测平台	预包被微孔板（T4 抗体）
适用物种	人、鼠、鼠、大鼠、山羊、绵羊、狗、兔子、牛、猴子、鸡、猪

试剂盒组成

• T4 抗体预包被微孔板
• T4 标准品（系列稀释浓度）
• 生物素偶联的 T4
• Avidin‑HRP 复合物
• HRP 底物 A 与 B（显色剂）
• 反应终止液
• 洗涤缓冲液
• 封板膜（防尘）

优势亮点

• 高灵敏度与特异性：EliKine™ 平台经过优化，能够在低至 10 ng/ml 的浓度上可靠检测 T4。
• 宽检测范围：一次实验即可覆盖 20 ng/ml – 320 ng/ml，满足临床前研究及药理学筛选的需求。
• 多物种兼容：同一套试剂盒可用于人及常见实验动物，降低实验室库存管理成本。
• 低背景噪声：经过严格的抗体筛选，未观察到显著交叉反应。
• 操作便利：配套的封板膜与预包装试剂减少了实验室准备时间。

实验操作要点（建议）

• 所有试剂须在室温（约 22 °C）平衡 30 分钟后使用。
• 使用一次性吸头，避免交叉污染。
• 未使用的板条请立即放回原包装，密封后在 4 °C 保存。
• 混匀是获得可重复结果的关键，推荐使用低速振荡器或每 10 分钟手动轻摇。
• 样品与标准品建议采用双孔或三孔复孔方式，提高数据可靠性。
• 不同批号的试剂不可混用，超过有效期的试剂请勿使用。

储存与运输

• 未开封试剂盒：2 °C–8 °C 保存。
• 启用后：各组分请参照随包装提供的说明书进行分装、短期冷藏或冷冻保存。
• 运输条件：使用蓝色冰袋冷链运输，确保全程温度控制。

使用声明

本产品仅限于科研用途，不得用于人类临床诊断或治疗。使用本套件进行的任何实验均应遵守当地法律法规及知识产权规定。因使用本产品可能产生的专利侵权或其他违规行为，供应方不承担任何责任。

产品规格与报价

包装规格	单价（人民币）	交付周期
48 T	1,598.00	期货（4 天内发货）
96 T	2,558.00	期货（4 天内发货）

背景信息

血清总甲状腺素（T4）是评估甲状腺功能的关键指标。血清中约 99% 的 T4 与甲状腺素结合球蛋白（TBG）结合，只有游离 T4（fT4）能够直接反映甲状腺的生理活性。通过本套件可准确定量血清总 T4，为药物代谢、内分泌干扰及疾病模型研究提供可靠数据支撑。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Thyroxine (T4) ELISA Kit
Chinese Name: T4 ELISA KIT Thyroid Hormone ELISA Kit (Human)
Catalog Number: KTE0007-2

Applications

• Product Category: Core Products → Cell Analysis → ELISA
• Primary Application: ELISA (Competitive Method)
• Main Analyte: Serum Total Thyroxine (T4)
• Assay Type: ELISA (Competitive Method)
• Sample Types: Serum, Plasma, Other Biological Fluids

Key Specifications

Detection Range	20 ng/ml – 320 ng/ml
Sensitivity	10 ng/ml
Cross Reactivity	No significant cross-reactivity or background interference observed
Competitor Reagent	Biotin-conjugated T4
Assay Platform	Pre-coated Microplate (T4 Antibody)
Species Reactivity	Human, Mouse, Rat, Goat, Sheep, Dog, Rabbit, Bovine, Monkey, Chicken, Porcine

Kit Components

• T4 Antibody Pre-coated Microplate
• T4 Standard (serial dilution concentrations)
• Biotin-conjugated T4
• Avidin-HRP Conjugate
• HRP Substrate A & B (Chromogen)
• Stop Solution
• Wash Buffer
• Plate Sealer (Dust-proof)

Key Advantages

• High Sensitivity and Specificity: The EliKine™ platform is optimized for reliable detection of T4 at concentrations as low as 10 ng/ml.
• Wide Detection Range: Covers 20 ng/ml – 320 ng/ml in a single assay, meeting the needs of preclinical research and pharmacological screening.
• Multi-Species Compatibility: A single kit can be used for humans and common laboratory animals, reducing inventory management costs.
• Low Background Noise: Rigorous antibody screening ensures no significant cross-reactivity is observed.
• Convenient Operation: Included plate sealer and pre-packaged reagents reduce laboratory preparation time.

Assay Procedure Notes (Recommendations)

• All reagents must be equilibrated to room temperature (approximately 22 °C) for 30 minutes before use.
• Use disposable pipette tips to avoid cross-contamination.
• Immediately return unused strips to the original packaging, seal, and store at 4 °C.
• Thorough mixing is critical for reproducible results; use a low-speed shaker or gently shake manually every 10 minutes.
• It is recommended to run samples and standards in duplicate or triplicate wells to improve data reliability.
• Do not mix reagents from different lot numbers. Do not use reagents beyond their expiration date.

Storage and Shipping

• Unopened Kit: Store at 2 °C – 8 °C.
• After Opening: Aliquot components as instructed in the provided manual for short-term refrigeration or freezing.
• Shipping Conditions: Shipped with blue ice packs under cold chain conditions to ensure temperature control throughout transit.

Intended Use Statement

This product is intended for research use only. It is not for use in human clinical diagnosis or therapy. Any experiments conducted using this kit must comply with local laws, regulations, and intellectual property rights. The supplier assumes no liability for patent infringement or other violations resulting from the use of this product.

Specifications and Pricing

Package Size	Unit Price (RMB)	Delivery Time
48 T	1,598.00	Stock (Ships within 4 days)
96 T	2,558.00	Stock (Ships within 4 days)

Background Information

Serum total thyroxine (T4) is a key indicator for assessing thyroid function. Approximately 99% of T4 in serum is bound to thyroxine-binding globulin (TBG); only free T4 (fT4) directly reflects the physiological activity of the thyroid gland. This kit enables accurate quantification of serum total T4, providing reliable data support for drug metabolism studies, endocrine disruption research, and disease model investigations.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Yakeyin Company for specific details.