人B细胞活化因子 ELISA定量试剂盒(BAFF/BlyS/

产品概述

欢迎使用EliKine™ 人B细胞活化因子(BAFF/BlyS/TNFSF13B) ELISA定量试剂盒（货号：KTE3122-1）。本试剂盒专为精准定量人血清、血浆及细胞培养上清样本中的BAFF蛋白浓度而设计，采用经典的夹心法ELISA原理，具备优异的检测性能与可靠性，是免疫学、细胞生物学及自身免疫疾病研究领域的理想工具。

核心特性与优势

• 高灵敏度与宽检测范围：检测灵敏度达31.25 pg/mL，线性检测范围为31.25 pg/mL至2,000 pg/mL，可满足低丰度样本的精准检测需求。
• 卓越的特异性：经严格验证，对目标蛋白类似物无明显交叉反应或背景干扰，确保检测结果的准确性。
• 即用型优化组分：预包被微孔板、冻干标准品及优化缓冲液体系，简化操作流程，缩短实验时间。
• 多样本类型兼容：适用于人源细胞培养上清、血清、血浆等多种生物样本，应用场景灵活。

产品背景

B细胞活化因子（BAFF，亦称BLys或TNFSF13B）是肿瘤坏死因子配体超家族成员，在B细胞的增殖、分化及存活中发挥核心调控作用。其表达水平与免疫稳态密切相关：BAFF不足可能导致免疫缺陷，而过量表达则与系统性红斑狼疮、类风湿性关节炎等自身免疫疾病的发生发展相关。本试剂盒通过双抗体夹心法精准捕获并定量BAFF蛋白，为免疫机制研究及疾病生物标志物分析提供可靠数据支持。

试剂盒组分（详细列表）

组分名称	规格	状态
人BAFF预包被微孔板	48T/96T	即用型
人BAFF标准品（冻干）	1支	使用前重建
标准品稀释液	1瓶	即用型
检测抗体（100×）	1支	使用前稀释
链霉亲和素-HRP（100×）	1支	使用前稀释
TMB底物溶液	1瓶	避光保存
终止液	1瓶	即用型
洗涤缓冲液（20×）	1瓶	使用前稀释
板盖	3片	一次性使用

技术参数

• 检测方法：夹心法ELISA
• 反应种属：人
• 样本类型：细胞培养上清、血清、血浆（建议避免溶血或高脂样本）
• 检测波长：450 nm（参考波长570 nm或630 nm）
• 复孔建议：推荐标准品及样本均做双孔或三孔检测以提高数据可靠性

保存与运输

• 保存条件：未开封试剂盒避光保存于2-8℃，有效期24个月；启用后请参照说明书分装保存各组分。
• 运输条件：全程蓝冰低温运输，确保试剂活性。

注意事项

• 请勿混合不同批号试剂或使用过期组分。
• 使用前所有试剂需室温平衡30分钟，充分混匀后使用。
• 实验操作需使用一次性吸头，避免交叉污染。
• 未使用的板条需立即密封并4℃保存。
• 推荐使用低速振荡器或定期手动摇匀以确保反应均匀性。

订购信息

规格	货号	价格（元）	货期
48 T	KTE3122-1	1598.0	请咨询
96 T	KTE3122-1	2558.0	期货（5个工作日内发货）

警告：本产品仅用于科学研究，不适用于临床诊断或人体直接使用。使用者应确保应用符合相关法规及知识产权要求。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Welcome to the EliKine™ Human B-Cell Activating Factor (BAFF/BLyS/TNFSF13B) ELISA Quantitation Kit (Cat. No.: KTE3122-1). This kit is specifically designed for the accurate quantitation of BAFF protein concentration in human serum, plasma, and cell culture supernatant samples. Utilizing the classic sandwich ELISA principle, it offers excellent detection performance and reliability, making it an ideal tool for research in immunology, cell biology, and autoimmune diseases.

Core Features & Advantages

• High Sensitivity & Broad Detection Range: Detection sensitivity reaches 31.25 pg/mL, with a linear detection range from 31.25 pg/mL to 2,000 pg/mL, meeting the needs for precise quantitation of low-abundance samples.
• Excellent Specificity: Rigorously validated to show no significant cross-reactivity or background interference with target protein analogs, ensuring accurate results.
• Ready-to-Use Optimized Components: Pre-coated microplate, lyophilized standard, and optimized buffer system simplify the operation process and reduce experimental time.
• Compatibility with Diverse Sample Types: Suitable for various biological samples including human cell culture supernatant, serum, and plasma, offering flexible application scenarios.

Product Background

B-cell activating factor (BAFF, also known as BLyS or TNFSF13B) is a member of the tumor necrosis factor ligand superfamily and plays a central regulatory role in the proliferation, differentiation, and survival of B cells. Its expression level is closely related to immune homeostasis: BAFF deficiency may lead to immunodeficiency, while overexpression is associated with the development and progression of autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis. This kit accurately captures and quantifies BAFF protein via a dual-antibody sandwich method, providing reliable data support for immunological mechanism research and disease biomarker analysis.

Kit Components (Detailed List)

Component Name	Specification	Status
Human BAFF Pre-coated Microplate	48T/96T	Ready to Use
Human BAFF Standard (Lyophilized)	1 vial	Reconstitute before use
Standard Diluent	1 bottle	Ready to Use
Detection Antibody (100×)	1 vial	Dilute before use
Streptavidin-HRP (100×)	1 vial	Dilute before use
TMB Substrate Solution	1 bottle	Store protected from light
Stop Solution	1 bottle	Ready to Use
Wash Buffer (20×)	1 bottle	Dilute before use
Plate Sealers	3 pieces	For single use

Technical Parameters

• Assay Method: Sandwich ELISA
• Reactivity: Human
• Sample Types: Cell culture supernatant, serum, plasma (Avoid hemolyzed or highly lipemic samples)
• Detection Wavelength: 450 nm (Reference wavelength: 570 nm or 630 nm)
• Replicate Recommendation: Duplicate or triplicate measurements for standards and samples are recommended to improve data reliability.

Storage & Shipping

• Storage Conditions: Store the unopened kit protected from light at 2-8°C; valid for 24 months. After opening, aliquot and store components according to the manual.
• Shipping Conditions: Shipped with blue ice throughout to ensure reagent activity.

Precautions

• Do not mix reagents from different lots or use expired components.
• Bring all reagents to room temperature and equilibrate for 30 minutes before use; mix thoroughly.
• Use disposable pipette tips to avoid cross-contamination.
• Immediately seal unused strips and store at 4°C.
• Use a low-speed shaker or periodic manual shaking to ensure reaction uniformity.

Ordering Information

Specification	Cat. No.	Price (RMB)	Availability
48 T	KTE3122-1	1598.0	Please Inquire
96 T	KTE3122-1	2558.0	Stock (Ships within 5 business days)

Warning: This product is for research use only, not for clinical diagnosis or direct human use. Users should ensure applications comply with relevant regulations and intellectual property rights.

Note: The above information is subject to change due to product optimization and upgrades. Please consult Yakeyin Company for specific details.