Inhibin beta A chain, Activin

重组人/小鼠/大鼠Activin A蛋白（无动物源）

产品概述

货号：PRP1088 | 产品名称：Human/Mouse/Rat Activin A Protein (Animal-Free)

本品为高纯度、高生物活性的重组Activin A蛋白，经大肠杆菌表达系统制备，无动物源成分，适用于严格的细胞培养与功能研究。该蛋白涵盖人、小鼠、大鼠三种物种的保守氨基酸序列（Gly311-Ser426），具有广泛的种属交叉反应性。

产品特性

• 高纯度：经SDS-PAGE检测，纯度 > 95%
• 低内毒素：LAL法检测，内毒素含量 < 0.1 EU/μg
• 高比活性：比活力 > 1.0 × 10³ IU/mg，ED₅₀ < 10 ng/mL（小鼠MPC-11细胞增殖抑制模型）
• 无动物源生产：表达宿主为大肠杆菌，避免动物源成分引入风险
• 冻干粉形态：经0.2 µm过滤冻干，便于运输与储存

技术参数

应用领域

Activin A作为TGF-β超家族成员，在细胞分化、增殖、凋亡及免疫调节中发挥关键作用：

• 干细胞定向分化（如红细胞分化、神经细胞分化）
• 肿瘤生物学研究（双向调控细胞生长）
• 炎症模型构建（如类风湿性关节炎、呼吸道疾病模型）
• 激素与生长因子信号通路研究

使用建议

• 复溶：推荐使用配套复溶缓冲液，浓度不低于100 μg/mL，避免剧烈涡旋
• 稀释：后续实验可用含载体蛋白的缓冲液（如0.1% BSA）进一步稀释
• 保存：冻干粉-20°C保存1年；复溶后2-8°C可保存1周，稀释后分装-20°C/-80°C保存3-6个月
• 运输：冰袋运输（蓝冰）

规格与定价

注意事项

本品仅供研究使用，不适用于临床诊断。使用前请离心处理冻干粉，避免反复冻融。具体应用需用户自行评估专利合规性。

参考文献序列：
人：UniProt P08476 | 小鼠：UniProt Q04998 | 大鼠：UniProt P18331

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human/Mouse/Rat Activin A Protein (Animal‑Free)

Product Overview

Catalog No.: PRP1088 | Product Name: Human/Mouse/Rat Activin A Protein (Animal‑Free)

This product is a high‑purity, high‑bioactivity recombinant Activin A protein prepared using an Escherichia coli expression system and contains no animal‑derived components, making it suitable for stringent cell‑culture and functional studies. The protein encompasses the conserved amino‑acid region (Gly311‑Ser426) from human, mouse, and rat, providing broad cross‑species reactivity.

Product Features

• High purity: SDS‑PAGE analysis shows purity > 95%
• Low endotoxin: LAL assay, endotoxin content < 0.1 EU/µg
• High specific activity: Specific activity > 1.0 × 10³ IU/mg, ED₅₀ < 10 ng/mL (mouse MPC‑11 cell proliferation inhibition model)
• Animal‑free production: Host expression system is E. coli, avoiding introduction of animal‑derived materials
• Lyophilized powder form: Filtered through 0.2 µm and lyophilized for convenient shipping and storage

Technical Specifications

Applications

Activin A, a member of the TGF‑β superfamily, plays pivotal roles in cell differentiation, proliferation, apoptosis, and immune regulation:

• Directed stem‑cell differentiation (e.g., erythroid, neuronal lineages)
• Cancer biology research (bidirectional regulation of cell growth)
• Inflammatory model development (e.g., rheumatoid arthritis, respiratory disease models)
• Hormone and growth‑factor signaling pathway studies

Usage Recommendations

• Reconstitution: Use the supplied reconstitution buffer; prepare at a concentration ≥ 100 µg/mL and avoid vigorous vortexing
• Dilution: Further dilute in a carrier‑protein buffer (e.g., 0.1 % BSA) for downstream assays
• Storage: Lyophilized powder – store at ‑20 °C for up to 1 year; after reconstitution, store at 2‑8 °C for up to 1 week; aliquoted dilutions can be kept at ‑20 °C/‑80 °C for 3‑6 months
• Shipping: Transport on ice packs (blue ice)

Specifications & Pricing

Precautions

This product is intended for research use only and is not suitable for clinical diagnosis. Prior to use, centrifuge the lyophilized powder to remove particulates and avoid repeated freeze‑thaw cycles. Users must assess patent compliance for their specific applications.

Reference sequence identifiers:
Human: UniProt P08476 | Mouse: UniProt Q04998 | Rat: UniProt P18331

Note: The information above may change due to product optimization or upgrades. Please contact Yakeyin Co. for the most current details.