Granulocyte Chemotactic Protei

产品概述

产品名称：Granulocyte Chemotactic Protein-2 (GCP-2)，重组人CXCL6蛋白，His标签（无动物源）

商品编号：PRP1072

分类：核心产品 → 细胞因子及蛋白 → 细胞因子

关键属性

• 氨基酸序列：人源CXCL6 (Val40‑Asn114) (UniProt P80162)
• 蛋白长度：75个氨基酸，预测分子量约 8.97 kDa
• 表达宿主：大肠杆菌
• 标签：6×His
• 纯度：≥ 98 %（SDS‑PAGE 检测）
• 内毒素：≤ 0.1 EU/µg（LAL法）
• 活性评价：对转染人CXCR2的BaF3细胞的趋化作用，ED₅₀ < 10 ng/mL

应用领域

本产品主要用于 细胞培养 中的功能实验，尤其适用于研究：

• 中性粒细胞趋化与炎症信号传导
• 细菌感染（革兰氏阳性/阴性）耐受机制
• CXCR1/CXCR2 介导的肿瘤细胞增殖与转移（如骨肉瘤）
• 其它与 C‑X‑C 族趋化因子相关的细胞生物学研究

产品形态与使用说明

本品提供 冻干粉 形式，包装于无菌包装袋中。配套的复溶缓冲液为 pH 7.4 的无菌 PBS。

复溶与稀释

1. 使用配套的 Reconstitution Buffer（PBS pH 7.4）复溶，目标浓度 ≥ 100 µg/mL。
2. 复溶后建议先离心（10 000 × g，1 min）除去可能的沉淀。
3. 依据实验需求可在含有 0.1 % BSA、10 % FBS 或 5 % HSA 的缓冲体系中进一步稀释。

储存与运输

• 冻干状态：‑20 °C，保质期 1 年。
• 复溶后：2 °C – 8 °C 最多保存 1 周；若加入载体蛋白或稳定剂，可分装后在 ‑20 °C 或 ‑80 °C 保存 3‑6 个月。
• 避免反复冻融循环。
• 运输方式：冰袋冷链（蓝冰）确保全程低温。

规格与价格

规格	含量	单价（人民币）	库存状态	备注
规格1	5 µg	588	现货（次日发货）	适用于小规模功能验证
规格2	20 µg	1498	现货（次日发货）	中等实验量
规格3	100 µg	5998	请咨询	批量实验或预研
规格4	1 mg	请咨询	请咨询	大规模生产验证

检测指标

产品经严格的质量控制，检测项目包括：CXCL6 蛋白含量、纯度（SDS‑PAGE）、内毒素（LAL）以及生物活性（BaF3 趋化实验）。

法规与安全提示

本产品仅限 科研使用，不适用于临床或诊断。使用前请阅读并遵守所在机构的生物安全规范。

警告：产品信息供参考，不构成任何专利、许可或法规的豁免。因使用本品而可能产生的专利侵权或其他合规风险，由用户自行承担。

参考文献

• UniProt Entry: P80162 (CXCL6)
• GeneID: 6372
• 相关研究：CXCL6 在细菌感染与肿瘤转移中的功能（文献可在PubMed检索）

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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Product Name: Granulocyte Chemotactic Protein-2 (GCP-2), Recombinant Human CXCL6 Protein, His-tag (Animal-Free)

Catalog Number: PRP1072

Category: Core Products → Cytokines & Proteins → Cytokines

Key Attributes

• Amino Acid Sequence: Human CXCL6 (Val40‑Asn114) (UniProt P80162)
• Protein Length: 75 amino acids, Predicted Molecular Weight: ~8.97 kDa
• Expression Host: E. coli
• Tag: 6×His
• Purity: ≥ 98 % (by SDS‑PAGE)
• Endotoxin: ≤ 0.1 EU/µg (LAL method)
• Activity Assay: Chemotactic activity on BaF3 cells transfected with human CXCR2, ED₅₀ < 10 ng/mL

Applications

This product is primarily intended for functional assays in cell culture, particularly suitable for investigating:

• Neutrophil chemotaxis and inflammatory signaling
• Tolerance mechanisms in bacterial infections (Gram-positive/negative)
• CXCR1/CXCR2-mediated tumor cell proliferation and metastasis (e.g., osteosarcoma)
• Other cell biology studies related to the C‑X‑C chemokine family

Product Form and Usage Instructions

This product is supplied as a lyophilized powder in sterile packaging. The accompanying reconstitution buffer is sterile PBS, pH 7.4.

Reconstitution and Dilution

1. Reconstitute using the provided Reconstitution Buffer (PBS pH 7.4) to a target concentration ≥ 100 µg/mL.
2. After reconstitution, brief centrifugation (10,000 × g, 1 min) is recommended to remove any potential precipitate.
3. For experimental use, further dilution can be performed in buffer systems containing 0.1 % BSA, 10 % FBS, or 5 % HSA.

Storage and Shipping

• Lyophilized State: Store at ‑20 °C, stable for 1 year.
• After Reconstitution: Store at 2 °C – 8 °C for up to 1 week; when supplemented with a carrier protein or stabilizer, aliquot and store at ‑20 °C or ‑80 °C for 3‑6 months.
• Avoid repeated freeze-thaw cycles.
• Shipping Method: Shipped on ice packs (blue ice) to maintain a cold chain.

Specifications and Pricing

Specification	Content	Price (RMB)	Stock Status	Remarks
Specification 1	5 µg	588	In Stock (Ships Next Day)	Suitable for small-scale functional validation
Specification 2	20 µg	1498	In Stock (Ships Next Day)	Medium experimental scale
Specification 3	100 µg	5998	Please Inquire	For batch experiments or pre-research
Specification 4	1 mg	Please Inquire	Please Inquire	For large-scale production validation

Quality Control

The product undergoes rigorous quality control, including testing for: CXCL6 protein content, purity (SDS‑PAGE), endotoxin level (LAL), and biological activity (BaF3 chemotaxis assay).

Regulatory and Safety Information

This product is intended for Research Use Only, not for clinical or diagnostic applications. Please read and adhere to your institution's biosafety guidelines before use.

Warning: Product information is for reference only and does not constitute any exemption from patents, licenses, or regulations. Users assume all risks associated with potential patent infringement or other compliance issues arising from the use of this product.

References

• UniProt Entry: P80162 (CXCL6)
• GeneID: 6372
• Related Research: Function of CXCL6 in bacterial infection and tumor metastasis (literature searchable via PubMed)

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Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Yakind for specific details.