FGFK, 重组人成纤维细胞生长因子20, 重组蛋白,细胞因

重组人成纤维细胞生长因子20 (FGF-20) | 无动物源，His标签

货号：PRP1924

产品名称：重组人FGF-20蛋白，His标签（无动物源）

英文全称：Human FGF-20 Protein, His tag (Animal-Free)

产品概述

本品为一种高纯度重组人源成纤维细胞生长因子20（FGF-20），采用无动物组分表达系统在大肠杆菌中表达，C末端带有His标签。该蛋白经过严格质控，适用于细胞培养及相关生命科学研究，尤其在神经发育与功能研究领域具有重要应用价值。

关键特性

• 高纯度：经SDS-PAGE验证，纯度 >98%
• 低内毒素：LAL法检测内毒素 <0.1 EU/µg
• 无动物源表达：生产过程无动物组分，降低外源因子风险
• 生物活性：ED₅₀范围为1.3–3.2 ng/mL，可诱导3T3细胞增殖，比活性 >2×10⁵ IU/mg

技术参数

• 种属来源：人
• 表达宿主：大肠杆菌
• 蛋白序列：Pro3-Ile211（UniProt: Q9NP95）
• 分子量：约24 kDa
• 标签类型：C末端His标签
• 产品形式：冻干粉（冻干缓冲液：PBS, pH 8.0）

应用方向

• 细胞增殖与分化研究
• 神经细胞培养与存活实验
• FGF/FGFR信号通路机制研究
• 多巴胺能神经元相关实验

使用建议

• 复溶：推荐使用配套复溶缓冲液，浓度不低于100 µg/mL
• 保存：冻干粉-20℃可保存1年；复溶后2-8℃可保存1周
• 长期储存：建议在含载体蛋白（如0.1% BSA）的缓冲液中分装保存于-20℃或-80℃，避免反复冻融

规格与价格

规格	价格（元）	货期
5 µg	588	现货，次日发货
20 µg	1498	现货，次日发货
100 µg	4539	请咨询
1 mg	请咨询	请咨询

背景信息

FGF-20是成纤维细胞生长因子家族成员，分子量约23.5 kDa，由211个氨基酸组成。其主要在小胶质细胞中表达，并通过FGF/FGFR信号通路参与中枢神经系统发育与功能调节，尤其对多巴胺能神经元的存活具有调控作用。

注意：本品仅供科研使用，不适用于人体或临床诊断。用户应确保使用方式不违反相关专利或许可规定。

运输条件：蓝冰冰袋运输

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Fibroblast Growth Factor 20 (FGF‑20) | Animal‑Free, His Tag

Item No.: PRP1924

Product Name: Recombinant Human FGF‑20 Protein, His Tag (Animal‑Free)

English Full Name: Human FGF‑20 Protein, His tag (Animal‑Free)

Product Overview

This product is a high‑purity recombinant human fibroblast growth factor 20 (FGF‑20) expressed in *Escherichia coli* using an animal‑component‑free expression system, featuring a C‑terminal His tag. The protein undergoes stringent quality control and is suitable for cell culture and related life‑science research, especially valuable in studies of neural development and function.

Key Characteristics

• High Purity: Verified by SDS‑PAGE, purity >98%
• Low Endotoxin: LAL assay endotoxin <0.1 EU/µg
• Animal‑Free Expression: Production process free of animal‑derived components, reducing risk of exogenous factors
• Biological Activity: ED₅₀ range 1.3–3.2 ng/mL, capable of inducing 3T3 cell proliferation, specific activity >2×10⁵ IU/mg

Technical Specifications

• Species: Human
• Expression Host: *Escherichia coli*
• Protein Sequence: Pro3‑Ile211 (UniProt: Q9NP95)
• Molecular Weight: Approximately 24 kDa
• Tag Type: C‑terminal His tag
• Product Form: Lyophilized powder (Lyophilization buffer: PBS, pH 8.0)

Application Areas

• Cell proliferation and differentiation studies
• Neuronal cell culture and survival assays
• FGF/FGFR signaling pathway mechanism studies
• Dopaminergic neuron‑related experiments

Recommended Usage

• Reconstitution: Recommended to use the supplied reconstitution buffer, at a concentration ≥100 µg/mL
• Storage: Lyophilized powder stable at ‑20 °C for 1 year; reconstituted solution stable at 2–8 °C for 1 week
• Long‑term storage: Aliquot in buffer containing carrier protein (e.g., 0.1% BSA) and store at ‑20 °C or ‑80 °C, avoiding repeated freeze‑thaw cycles

Specifications and Pricing

Specification	Price (CNY)	Lead Time
5 µg	588	In stock, next‑day shipping
20 µg	1498	In stock, next‑day shipping
100 µg	4539	Please inquire
1 mg	Please inquire	Please inquire

Background Information

FGF‑20 is a member of the fibroblast growth factor family, with a molecular weight of approximately 23.5 kDa and composed of 211 amino acids. It is primarily expressed in microglial cells and participates in central nervous system development and functional regulation via the FGF/FGFR signaling pathway, notably influencing the survival of dopaminergic neurons.

Note: This product is for research use only and is not intended for human or clinical diagnostic applications. Users should ensure that their use does not infringe any relevant patents or licensing agreements.

Shipping Condition: Shipped on dry ice

Note: The above information may change due to product optimization or upgrades; please contact Yakeyin Co. for the most current details.