Monocyte Chemotactic Protein-1

重组人CCL2蛋白 (His标签，无动物源)

产品货号: PRP1908

产品名称: Human CCL2 Protein, His tag (Animal-Free) / 重组人趋化因子配体2，His-标签（无动物源）

产品概述

本产品是一种高纯度、重组表达的人源单核细胞趋化蛋白-1 (MCP-1/CCL2)。该蛋白在大肠杆菌系统中表达，并采用无动物源工艺生产，有效降低了病毒污染风险，为细胞培养及相关生物医学研究提供了可靠的工具。

关键特性

• 高纯度: 经SDS-PAGE验证，纯度 > 95%。
• 低内毒素: 每微克蛋白内毒素含量 < 0.1 EU (LAL法测定)，适用于对内毒素敏感的实验。
• 已验证生物活性: 通过其对转染人CCR2A的BaF3细胞的趋化吸引实验测定，ED₅₀ < 20 ng/mL。
• 无动物源生产: 整个生产过程避免使用任何动物源性成分，提升了产品的安全性。
• 明确的分子量: 重组蛋白由75个氨基酸组成，预测分子量为8.5 kDa，实际观测分子量约为9.49 kDa。

产品描述

重组人CCL2蛋白包含人CCL2 (UniProt ID: P13500) 的Gln24-Lys98氨基酸序列，并在N端融合了6×His标签，便于检测和纯化。CCL2是一种重要的趋化因子，主要由单核细胞、巨噬细胞和树突状细胞分泌，通过与其受体CCR4等结合，在免疫细胞（如单核细胞、记忆T细胞）的募集、炎症反应、血管生成及细胞稳态维持等生理病理过程中发挥关键作用。

产品形式与规格

本品为经0.2 µm过滤PBS (pH 7.4) 缓冲液冻干而成的粉末。

使用说明

• 复溶: 开盖前请先离心。推荐使用配套的复溶缓冲液将冻干粉溶解至浓度不低于100 µg/mL，后续可根据实验需求进行稀释。
• 保存: 冻干粉可在-20°C下稳定保存1年。复溶后，在2-8°C下可保存1周。若在含有载体蛋白（如0.1% BSA、10% FBS、5% HSA）的缓冲液中稀释后分装，于-20°C或-80°C下可保存3-6个月，建议避免反复冻融。

运输与警告

运输条件: 冰袋运输（蓝冰）。

警告: 本品仅供研究使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定。

相关资源

蛋白质序列信息请参考UniProt数据库：P13500.1

如有任何疑问或特殊需求，欢迎随时联系我们。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human CCL2 Protein (His tag, Animal-Free)

Product Catalog No.: PRP1908

Product Name: Human CCL2 Protein, His tag (Animal-Free) / Recombinant Human Chemokine Ligand 2, His‑tag (Animal-Free)

Product Overview

This product is a high‑purity, recombinantly expressed human monocyte chemoattractant protein‑1 (MCP‑1/CCL2). The protein is produced in an Escherichia coli system using an animal‑free manufacturing process, effectively reducing the risk of viral contamination and providing a reliable tool for cell culture and related biomedical research.

Key Features

• High Purity: Verified by SDS‑PAGE, purity > 95%.
• Low Endotoxin: Endotoxin level < 0.1 EU per µg protein (LAL assay), suitable for endotoxin‑sensitive applications.
• Validated Bioactivity: Chemotaxis assay using BaF3 cells transfected with human CCR2A showed an ED₅₀ < 20 ng/mL.
• Animal‑Free Production: The entire production process avoids any animal‑derived components, enhancing product safety.
• Defined Molecular Weight: The recombinant protein comprises 75 amino acids, with a predicted molecular weight of 8.5 kDa and an observed molecular weight of approximately 9.49 kDa.

Product Description

The recombinant human CCL2 protein contains the Gln24‑Lys98 amino‑acid sequence of human CCL2 (UniProt ID: P13500) with an N‑terminal 6×His tag for convenient detection and purification. CCL2 is a pivotal chemokine secreted primarily by monocytes, macrophages, and dendritic cells. By binding to receptors such as CCR4, it plays essential roles in the recruitment of immune cells (e.g., monocytes, memory T cells), inflammation, angiogenesis, and maintenance of cellular homeostasis.

Product Form and Specifications

The product is supplied as a lyophilized powder reconstituted in 0.2 µm‑filtered PBS (pH 7.4).

Usage Instructions

• Reconstitution: Centrifuge the vial before opening. It is recommended to dissolve the lyophilized powder in the supplied reconstitution buffer to a concentration of at least 100 µg/mL, then dilute further as required for your experiments.
• Storage: The lyophilized powder is stable at –20 °C for up to 1 year. After reconstitution, store at 2–8 °C for up to 1 week. If aliquoted in a buffer containing carrier protein (e.g., 0.1% BSA, 10% FBS, or 5% HSA), the solution can be kept at –20 °C or –80 °C for 3–6 months; avoid repeated freeze‑thaw cycles.

Transportation and Warning

Transport Conditions: Shipped on ice packs (blue ice).

Warning: This product is for research use only and is not intended for human or clinical diagnostic applications. Users must ensure that its use does not infringe any patents or licensing agreements.

Related Resources

For protein sequence information, refer to the UniProt database: P13500.1

If you have any questions or special requirements, please feel free to contact us.

Note: The information above may change due to product optimization or other factors. For the most current details, please consult Yakeyin Company.