IL-T, 重组人白介素-15, IL-15, IL 15,

重组人白介素-15（IL-15），His标签（无动物源）

产品概述

本品为重组人白介素-15（IL-15）蛋白，采用无动物源表达系统在大肠杆菌中表达，并融合N端6×His标签，适用于各类细胞培养及相关免疫学研究应用。产品经严格质控，具备高纯度和稳定的生物活性，可满足科研实验中对细胞因子功能研究的需要。

产品详情

• 货号: PRP1063
• 蛋白名称: Human IL-15 protein, His tag (Animal-Free)
• 种属来源: 人源
• 表达宿主: 大肠杆菌表达系统
• 蛋白长度: 114个氨基酸，预测分子量约为12.7 kDa
• 纯度: > 95%（SDS-PAGE检测）
• 标签: N端6×His标签
• 活性（比活力）: > 2 × 10⁶ IU/mg，ED50 < 3 ng/mL（CTLL-2细胞增殖实验）
• 内毒素水平: < 0.01 EU/μg（LAL法检测）

产品应用建议

本品在多种免疫细胞功能研究中具有广泛用途，包括但不限于：

• NK细胞与T细胞的增殖、分化与存活研究
• 先天性免疫与适应性免疫应答机制分析
• 细胞趋化与炎症反应相关实验

产品形式与复溶方法

本品为经0.2 µm过滤并冻干后的蛋白质粉末，缓冲体系为1X PBS，pH 8.0。

复溶建议：使用无菌ddH₂O复溶，浓度建议不低于100 μg/mL。复溶后请在室温静置至少20分钟，避免振荡或剧烈涡旋，以保持蛋白生物活性。

保存与运输条件

• 保存: 冻干粉可于-20℃保存一年；复溶后于2-8℃可保存一周。如需长期保存，建议在含载体蛋白（如0.1% BSA、10% FBS或5% HSA）的缓冲液中进一步稀释后分装，并保存于-20℃或-80℃，避免反复冻融。
• 运输: 采用蓝冰（冰袋）低温运输，保障产品稳定性。

注意事项

使用前请以3000 rpm离心5分钟，确保产品完全沉降。本品仅供科研使用，不适用于人体或临床诊断。用户应自行负责确保其应用不违反相关专利或许可规定。

背景信息

人白介素-15（IL-15）是一种多效性细胞因子，在免疫调节中发挥核心作用。它参与NK细胞与T细胞的发育、存活及功能调控，并在单核细胞和树突状细胞中诱导趋化因子产生，具有重要的生理与病理意义。

规格与价格

别名: IL-T, IL-15, IL 15, IL15, 重组人白介素-15
UniProt编号: P40933

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Interleukin-15 (IL-15), His Tag (Animal-Free)

Product Overview

This product is a recombinant human interleukin‑15 (IL‑15) protein, expressed in Escherichia coli using an animal‑free expression system and fused with an N‑terminal 6×His tag. It is suitable for a variety of cell culture and related immunological research applications. The product undergoes stringent quality control, possesses high purity and stable bioactivity, and meets the requirements for cytokine functional studies in research.

Product Details

• Catalog No.: PRP1063
• Protein Name: Human IL-15 protein, His tag (Animal-Free)
• Species Origin: Human
• Expression Host: Escherichia coli expression system
• Protein Length: 114 amino acids, predicted molecular weight ~12.7 kDa
• Purity: >95% (SDS‑PAGE)
• Tag: N‑terminal 6×His tag
• Activity (specific activity): >2 × 10⁶ IU/mg, ED₅₀ < 3 ng/mL (CTLL‑2 proliferation assay)
• Endotoxin Level: <0.01 EU/μg (LAL assay)

Recommended Applications

This product has broad utility in various immune cell functional studies, including but not limited to:

• NK and T cell proliferation, differentiation, and survival studies
• Analysis of innate and adaptive immune response mechanisms
• Cell chemotaxis and inflammation‑related assays

Product Formulation and Reconstitution

The product is a lyophilized protein powder filtered through a 0.2 µm membrane, supplied in 1× PBS, pH 8.0.

Reconstitution Recommendation: Reconstitute with sterile ddH₂O to a concentration of no less than 100 µg/mL. After reconstitution, let stand at room temperature for at least 20 minutes; avoid vortexing or vigorous shaking to preserve protein bioactivity.

Storage and Shipping Conditions

• Storage: Lyophilized powder is stable at –20 °C for up to one year; reconstituted solution can be stored at 2–8 °C for one week. For long‑term storage, dilute into a buffer containing carrier protein (e.g., 0.1 % BSA, 10 % FBS, or 5 % HSA), aliquot, and store at –20 °C or –80 °C, avoiding repeated freeze‑thaw cycles.
• Shipping: Shipped on ice packs (blue ice) to maintain low temperature and product stability.

Precautions

Prior to use, centrifuge at 3000 rpm for 5 minutes to ensure complete sedimentation. This product is for research use only and is not intended for human or clinical diagnostic applications. Users are responsible for ensuring that their use does not infringe any patents or licensing agreements.

Background

Human interleukin‑15 (IL‑15) is a pleiotropic cytokine that plays a central role in immune regulation. It is involved in the development, survival, and functional regulation of NK and T cells, and induces chemokine production in monocytes and dendritic cells, bearing significant physiological and pathological relevance.

Specifications and Pricing

Synonyms: IL‑T, IL‑15, IL 15, IL15, recombinant human interleukin‑15
UniProt ID: P40933

Note: The above information is subject to change due to product optimization or upgrades; please contact Yakoin for the most current details.