Growth/Differentiation Factor-

重组人生长分化因子7 (GDF7/BMP-12) 蛋白，His标签

产品概述

Human GDF7 Protein, His tag（货号：PRP1230）是一种重组表达的人源生长分化因子7（GDF-7/BMP-12）蛋白片段，带His-TrxA融合标签。本品选用大肠杆菌表达系统，经SDS-PAGE验证纯度高于95%，适用于细胞培养及相关体外研究。

关键特性

• 高纯度：经SDS-PAGE检测，纯度 > 95%
• 低内毒素：LAL法检测，每微克蛋白内毒素 < 0.1 EU
• 蛋白结构：由129个氨基酸组成（Thr322-Arg450），预测分子量为14.2 kDa（融合TrxA后约33.8 kDa）
• 冻干形式：冻干缓冲液为无菌PBS，pH 7.5，便于复溶与保存

应用方向

GDF7属于TGF-β超家族成员，在神经系统发育及软骨、韧带组织修复中具有调控作用。本品适用于：

• 神经细胞分化研究
• 组织工程与再生医学相关体外实验
• 细胞信号通路机制研究

使用说明

• 复溶建议：推荐使用配套复溶缓冲液，复溶浓度不低于100 µg/mL
• 保存条件：冻干粉建议保存于-20℃以下；复溶后可在4℃短期保存（2-7天），长期储存需分装并置于-20℃以下，可添加0.1% HSA或BSA作为稳定剂
• 注意事项：使用前请离心，避免反复冻融

规格与价格

背景信息

GDF7（Growth/Differentiation Factor-7，也称BMP-12）在胚胎中枢神经系统发育中由顶板细胞选择性表达，对神经元身份认定及软骨、韧带组织的形成与修复具有重要作用。其基因ID为151449，UniProt编号为Q7Z4P5。

注意：本品仅限于科研用途，不用于人体或临床诊断。使用前请查阅相关文献并遵守所在机构的生物安全规范。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Growth/Differentiation Factor 7 (GDF7/BMP-12) Protein, His Tag

Product Overview

Human GDF7 Protein, His tag (Catalog No.: PRP1230) is a recombinant expressed human growth/differentiation factor 7 (GDF‑7/BMP‑12) protein fragment with a His‑TrxA fusion tag. This product utilizes an Escherichia coli expression system, and SDS‑PAGE analysis confirms purity > 95%, suitable for cell culture and related in‑vitro studies.

Key Features

• High purity: SDS‑PAGE analysis shows purity > 95%
• Low endotoxin: LAL assay, endotoxin < 0.1 EU per µg protein
• Protein structure: composed of 129 amino acids (Thr322‑Arg450), predicted molecular weight 14.2 kDa (≈33.8 kDa after TrxA fusion)
• Lyophilized form: lyophilization buffer is sterile PBS, pH 7.5, facilitating reconstitution and storage

Application Areas

GDF7 belongs to the TGF‑β superfamily and plays regulatory roles in nervous system development and cartilage/ligament tissue repair. This product is suitable for:

• Neuronal differentiation studies
• In‑vitro experiments related to tissue engineering and regenerative medicine
• Cell signaling pathway mechanistic studies

Usage Instructions

• Reconstitution recommendation: use the supplied reconstitution buffer; final concentration should be ≥100 µg/mL
• Storage conditions: lyophilized powder should be stored at ≤‑20 °C; after reconstitution, short‑term storage at 4 °C is acceptable (2–7 days). For long‑term storage, aliquot and store at ≤‑20 °C, optionally adding 0.1 % HSA or BSA as a stabilizer.
• Precautions: centrifuge before use and avoid repeated freeze‑thaw cycles

Specifications and Pricing

Background Information

GDF7 (Growth/Differentiation Factor‑7, also known as BMP‑12) is selectively expressed by roof plate cells during embryonic central nervous system development and plays critical roles in neuronal identity specification as well as cartilage and ligament formation and repair. Its Gene ID is 151449 and the UniProt accession number is Q7Z4P5.

Note: This product is for research use only and is not intended for human or clinical diagnostic applications. Please consult relevant literature and adhere to your institution’s biosafety regulations before use.

Note: The above information may be subject to change due to product optimization or upgrades; for the most current details, please contact YaKeYin Co.