IL-4 重组小鼠白介素-4 小鼠重组蛋白 细胞因子 PRP

重组小鼠白介素-4 (Mouse IL-4 Protein, His Tag, Animal-Free)

货号: PRP2117
产品类型: 细胞因子
种属来源: 小鼠 (Mus musculus)
表达系统: 人类胚胎肾细胞 (HEK293) 表达，无动物源成分
产品形式: 高纯度冻干粉

产品描述

本品是一种重组表达的小鼠白细胞介素-4 (IL-4) 蛋白。该蛋白序列来源于UniProt登录号P07750 (Met1-Ser140)，并在C末端融合有多聚组氨酸标签(His Tag)，采用无动物源(AF)表达系统制备，最大限度地降低了外源病原体风险。本品经严格纯化，SDS-PAGE检测纯度高于98%，为生命科学研究提供高可靠性保障。

产品特点与优势

• 高纯度: > 98% (SDS-PAGE 检测)
• 低内毒素: 每1μg蛋白质中内毒素(EU)含量 < 0.1 (LAL法检测)
• 无动物源表达: 采用HEK293细胞表达系统，避免外源动物病原体污染
• 明确的分子量: 还原性SDS-PAGE检测分子量约为15-19 kDa
• 多种包装规格: 提供5μg、20μg和100μg三种规格，满足不同实验需求

技术参数

基因ID: 16189
蛋白ID: P07750
蛋白长度: 118个氨基酸
预测分子量: 13.3 kDa
实际分子量: 15-19 kDa (还原性SDS-PAGE)
标签类型: C末端His标签
表达宿主: 人类胚胎肾细胞(HEK293)

生物活性背景

白细胞介素-4(IL-4)是一种多效性细胞因子，主要由活化的T淋巴细胞、嗜碱性粒细胞和肥大细胞产生。它具有典型的四α-螺旋束结构，含有三个链内二硫键。小鼠IL-4在免疫调节中发挥重要作用，包括诱导B淋巴细胞产生IgE、促进Th2细胞分化、调节同种型转换等。此外，IL-4在过敏反应、肿瘤免疫和自身免疫性疾病的研究中也具有重要意义。

使用说明

复溶方法: 推荐使用配套复溶缓冲液进行复溶，浓度不低于100μg/mL。可根据实验需求进一步稀释。
注意事项: 使用前请离心，避免反复冻融。
保存条件: 冻干粉可在-20℃以下长期保存；复溶后可在4℃保存2-7天，长期保存请置于-20℃以下，建议添加载体蛋白(0.1% HSA或BSA)。

运输与存储

运输条件: 冰袋运输(蓝冰)
保存条件: -20℃以下干燥环境保存

规格与价格

规格	价格 (￥)	货期
5 μg	588	现货（次日发货）
20 μg	1498	现货（次日发货）
100 μg	4516	现货（次日发货）

注意事项

警告: 本品仅供研究使用，不适用于人类或临床诊断。使用本产品可能涉及专利问题，请用户自行确认相关知识产权状况。

蛋白序列信息: 详见UniProt数据库(P07750) -

别名: IL-4; Interleukin 4; BCGF1; BSF1; 重组小鼠白介素-4

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Mouse Interleukin-4 (Mouse IL-4 Protein, His Tag, Animal-Free)

Catalog Number: PRP2117
Product Type: Cytokine
Species Source: Mouse (Mus musculus)
Expression System: Expressed in Human Embryonic Kidney (HEK293) cells, Animal-Free
Product Form: High Purity Lyophilized Powder

Product Description

This product is a recombinant Mouse Interleukin-4 (IL-4) protein. The protein sequence is derived from UniProt Accession P07750 (Met1-Ser140), with a polyhistidine tag (His Tag) fused at the C-terminus. It is produced using an Animal-Free (AF) expression system, minimizing the risk of exogenous pathogens. This product undergoes stringent purification and exhibits a purity of >98% as determined by SDS-PAGE, providing high reliability for life science research.

Features & Benefits

• High Purity: > 98% (by SDS-PAGE analysis)
• Low Endotoxin: < 0.1 EU per 1μg of protein (LAL method)
• Animal-Free Expression: Expressed in HEK293 cell system, avoiding contamination from exogenous animal pathogens
• Defined Molecular Weight: Approximately 15-19 kDa observed by reducing SDS-PAGE
• Multiple Packaging Sizes: Available in 5μg, 20μg, and 100μg sizes to meet various experimental needs

Technical Parameters

Gene ID: 16189
Protein ID: P07750
Protein Length: 118 amino acids
Predicted Molecular Weight: 13.3 kDa
Observed Molecular Weight: 15-19 kDa (by reducing SDS-PAGE)
Tag Type: C-terminal His Tag
Expression Host: Human Embryonic Kidney (HEK293) cells

Biological Activity Background

Interleukin-4 (IL-4) is a pleiotropic cytokine primarily produced by activated T lymphocytes, basophils, and mast cells. It features a characteristic four α-helical bundle structure with three intra-chain disulfide bonds. Mouse IL-4 plays a significant role in immune regulation, including inducing IgE production by B lymphocytes, promoting Th2 cell differentiation, and regulating isotype switching. Furthermore, IL-4 is important for research in allergies, tumor immunology, and autoimmune diseases.

Usage Instructions

Reconstitution: Reconstitution with the provided buffer to a concentration not less than 100μg/mL is recommended. Further dilution can be performed according to experimental requirements.
Precautions: Centrifuge briefly before use. Avoid repeated freeze-thaw cycles.
Storage Conditions: Lyophilized powder can be stored long-term at ≤ -20°C. After reconstitution, it can be stored at 4°C for 2-7 days. For long-term storage, store at ≤ -20°C, and addition of a carrier protein (e.g., 0.1% HSA or BSA) is suggested.

Shipping & Storage

Shipping Conditions: Shipped with ice packs (blue ice)
Storage Conditions: Store in a dry environment at ≤ -20°C

Specifications & Pricing

Size	Price (¥)	Availability
5 μg	588	In Stock (Ships Next Day)
20 μg	1498	In Stock (Ships Next Day)
100 μg	4516	In Stock (Ships Next Day)

Precautions

Warning: This product is for research use only and is not intended for human or clinical diagnostic use. Use of this product may involve patent issues; users are responsible for confirming relevant intellectual property status.

Protein Sequence Information: Refer to the UniProt database (P07750) -

Aliases: IL-4; Interleukin 4; BCGF1; BSF1; Recombinant Mouse Interleukin-4

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult AcroBiosystems for specific details.