DCUA, DFNA69, FPH2, FPHH, KL-1

重组人干细胞因子 (SCF) 蛋白，无标签 (Human SCF Protein, Tag Free)

产品货号：PRP1234

产品概述

本产品是一款由大肠杆菌表达系统重组生产的无标签人源干细胞因子 (SCF) 蛋白。其氨基酸序列对应于人SCF (UniProt ID: P21583) 的Glu26-Ala189片段，由164个氨基酸组成，预测分子量为18.6 kDa。产品为高纯度（>98%, SDS-PAGE检测）冻干粉末，每微克内毒素含量低于0.1 EU，严格的质量控制使其成为细胞培养研究的可靠选择。

产品特性

• 基因ID：9965
• 种属来源：人 (Human)
• 表达宿主：大肠杆菌 (E. coli)
• 蛋白长度：164个氨基酸
• 预测分子量：18.6 kDa
• 标签：无标签 (Tag Free)
• 纯度：> 98% (通过SDS-PAGE检测)
• 内毒素水平：< 0.1 EU/μg (LAL法测定)
• 产品形式：冻干粉
• 冻干缓冲液：无菌20 mM Tris, 50 mM NaCl, pH 8.0
• 活性：检测中 (Testing in Progress)

背景信息

干细胞因子 (Stem Cell Factor, SCF)，也被称为KIT配体，是一种由成纤维细胞和内皮细胞产生的关键细胞因子。它通过结合c-Kit受体，在造血功能、精子发生和黑色素生成中扮演重要角色，并能促进肥大细胞的粘附、迁移、增殖和存活。人源SCF与犬、猫、小鼠和大鼠的SCF具有79%-87%的氨基酸序列同源性，但对其小鼠细胞的生物活性较弱。

使用说明

• 复溶：推荐使用产品配套的复溶缓冲液 (Reconstitution Buffer) 进行复溶，复溶浓度建议不低于100 µg/mL。用户可根据后续实验需求进行进一步稀释。
• 注意事项：开盖使用前，请先离心。请注意合理的实验操作以避免蛋白降解。

保存与运输

• 保存建议：冻干粉形式建议保存于-20℃以下的干燥环境中。复溶后，可在4℃下保存2-7天；如需长期保存，请置于-20℃以下，并建议添加载体蛋白（如0.1% HSA或BSA），分装保存以避免反复冻融。
• 运输条件：冰袋运输（蓝冰）。

规格与价格

警告：本产品仅限于研究用途，不适用于人类或临床诊断。使用本产品不得违反任何专利或许可证规定，对于因此可能产生的专利侵权或其他违规行为，我们不承担任何责任。

相关资源：
蛋白序列信息请参考UniProt数据库：P21583

别名：
DCUA, DFNA69, FPH2, FPHH, KL-1, Kitl, MGF, SF, SHEP7, SLF, KIT ligand, SCF, 干细胞因子, 重组人干细胞因子（SCF）

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Stem Cell Factor (SCF) Protein, Tag-Free (Human SCF Protein, Tag Free)

Product Code: PRP1234

Product Overview

This product is a tag‑free recombinant human Stem Cell Factor (SCF) protein expressed in an Escherichia coli system. Its amino acid sequence corresponds to the human SCF (UniProt ID: P21583) segment Glu26‑Ala189, comprising 164 residues with a predicted molecular weight of 18.6 kDa. The product is a high‑purity (>98% by SDS‑PAGE) lyophilized powder, with endotoxin levels below 0.1 EU/µg. Rigorous quality control makes it a reliable choice for cell‑culture research.

Product Characteristics

• Gene ID: 9965
• Species: Human (Human)
• Expression Host: E. coli (E. coli)
• Protein Length: 164 amino acids
• Predicted Molecular Weight: 18.6 kDa
• Tag: Tag‑Free (Tag Free)
• Purity: > 98% (SDS‑PAGE)
• Endotoxin Level: < 0.1 EU/µg (LAL assay)
• Product Form: Lyophilized powder
• Lyophilization Buffer: Sterile 20 mM Tris, 50 mM NaCl, pH 8.0
• Activity: Testing in Progress

Background Information

Stem Cell Factor (SCF), also known as KIT ligand, is a pivotal cytokine produced by fibroblasts and endothelial cells. By binding the c‑Kit receptor, it plays essential roles in hematopoiesis, spermatogenesis, and melanogenesis, and promotes mast cell adhesion, migration, proliferation, and survival. Human SCF shares 79‑87 % amino‑acid sequence identity with canine, feline, murine, and rat SCF, but exhibits relatively weak bioactivity on mouse cells.

Instructions for Use

• Reconstitution: It is recommended to dissolve the product with the supplied Reconstitution Buffer to achieve a concentration of ≥100 µg/mL. Users may further dilute according to downstream experimental requirements.
• Precautions: Prior to opening, centrifuge the vial. Handle appropriately to minimize protein degradation.

Storage and Shipping

• Storage Recommendation: Store the lyophilized powder in a dry environment at ≤ ‑20 °C. After reconstitution, keep at 4 °C for 2–7 days; for long‑term storage, retain at ≤ ‑20 °C, optionally add a carrier protein (e.g., 0.1 % HSA or BSA), and aliquot to avoid repeated freeze‑thaw cycles.
• Shipping Conditions: Shipped on ice packs (blue ice).

Specifications and Pricing

Warning: This product is for research use only and is not intended for human or clinical diagnostic applications. Use of this product must not infringe any patents or licensing agreements; we assume no liability for any patent infringement or other violations that may arise.

Related Resources:
Protein sequence information can be found in the UniProt database: P21583

Aliases:
DCUA, DFNA69, FPH2, FPHH, KL-1, Kitl, MGF, SF, SHEP7, SLF, KIT ligand, SCF, Stem Cell Factor, Recombinant Human Stem Cell Factor (SCF)

Note: The above information is subject to change due to product optimization or upgrades; please contact Yakoin for the most current details.