SCF - Human SCF Protein, His t

产品概述

SCF - Human SCF Protein, His tag (Animal-Free)（货号：PRP1905）是一种重组表达的人源干细胞因子蛋白，采用无动物源表达系统生产，C端带有6×His标签，适用于细胞培养及相关研究应用。

产品详情

• 英文名称：Human SCF Protein, His tag (Animal-Free)
• 种属来源：人
• 表达宿主：大肠杆菌表达系统，无动物源性成分
• 氨基酸序列：来源于人SCF（UniProt: P21583，Thr25-Ala189）
• 蛋白长度与分子量：165个氨基酸，预测分子量为18.6 kDa，实际SDS-PAGE检测约为19.4 kDa
• 纯度：经SDS-PAGE检测，纯度 > 98%
• 活性（比活力）：> 5 × 10⁵ IU/mg，通过TF-1细胞增殖实验测定，ED₅₀ < 5 ng/mL
• 内毒素水平：LAL法检测，< 0.1 EU/μg

产品形式与复溶建议

本品为冻干粉末，由0.2 µm过滤的1X PBS（pH 7.4）溶液冻干制成。建议使用配套复溶缓冲液（Reconstitution Buffer）复溶至浓度不低于100 µg/mL，后续可根据实验需求进一步稀释。

保存与运输条件

• 保存条件：冻干粉-20°C保存可达1年；复溶后2–8°C可保存1周；稀释后（如含0.1% BSA、10% FBS或5% HSA）可分装存于-20°C或-80°C，避免反复冻融。
• 运输方式：冰袋（蓝冰）运输，保障蛋白稳定性。

背景信息

SCF（Stem Cell Factor）是c-kit受体的配体，广泛表达于成纤维细胞、内皮细胞、肥大细胞等多种细胞类型。它在造血干细胞的增殖、分化及与其他因子（如G-CSF、GM-CSF、IL-3）协同作用中具有重要功能。

应用说明

本品适用于细胞培养、信号通路研究、干细胞分化等体外实验，推荐在无血清或低血清培养体系中验证使用效果。

规格与价格

注意：本品仅供研究使用，不适用于人体或临床诊断。使用者应确保不违反相关专利或许可协议。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

SCF - Human SCF Protein, His tag (Animal-Free) (Catalog No.: PRP1905) is a recombinant human stem cell factor protein produced using an animal‑free expression system, featuring a C‑terminal 6×His tag, suitable for cell culture and related research applications.

Product Details

• English Name: Human SCF Protein, His tag (Animal-Free)
• Species Origin: Human
• Expression Host: Escherichia coli expression system, animal‑free components
• Amino Acid Sequence: Derived from human SCF (UniProt: P21583, Thr25‑Ala189)
• Protein Length and Molecular Weight: 165 amino acids, predicted molecular weight 18.6 kDa, observed by SDS‑PAGE ≈ 19.4 kDa
• Purity: > 98% as assessed by SDS‑PAGE
• Activity (Specific Activity): > 5 × 10⁵ IU/mg, determined by TF‑1 cell proliferation assay, ED₅₀ < 5 ng/mL
• Endotoxin Level: < 0.1 EU/μg by LAL assay

Product Format and Reconstitution Recommendation

The product is supplied as a lyophilized powder, prepared from 1 X PBS (pH 7.4) filtered through a 0.2 µm filter. Reconstitute with the provided Reconstitution Buffer to a concentration of at least 100 µg/mL; further dilutions can be made as required for specific experiments.

Storage and Shipping Conditions

• Storage Conditions: Lyophilized powder stable at –20 °C for up to 1 year; after reconstitution store at 2–8 °C for up to 1 week; diluted aliquots (e.g., containing 0.1% BSA, 10% FBS, or 5% HSA) can be stored at –20 °C or –80 °C, avoiding repeated freeze‑thaw cycles.
• Shipping Method: Transport on ice packs (blue ice) to maintain protein stability.

Background Information

Stem cell factor (SCF) is the ligand for the c‑Kit receptor and is widely expressed in fibroblasts, endothelial cells, mast cells, and numerous other cell types. It plays a critical role in the proliferation and differentiation of hematopoietic stem cells and synergizes with other cytokines such as G‑CSF, GM‑CSF, and IL‑3.

Application Notes

This product is suitable for cell culture, signaling pathway studies, and stem cell differentiation assays in vitro; performance is recommended to be validated under serum‑free or low‑serum culture conditions.

Specifications and Pricing

Note: This product is for research use only and is not intended for human or clinical diagnostic applications. Users should ensure compliance with any relevant patents or licensing agreements.

Note: The above information may be subject to change due to product optimization or upgrades; please contact Yakeyin Co. for the most current details.