MVCD1, VEGF, VEGF165, VPF, Vas

重组人血管内皮生长因子165 (VEGFA) - 人源细胞表达，高纯度

货号：PRP100240

产品名称：Human VEGF165 protein

产品概述

重组人血管内皮生长因子165 (VEGFA) 是通过人源细胞表达系统生产的无标签重组蛋白。该蛋白的氨基酸序列来源于人源VEGF165 isoform (P15692-4)，由移除信号肽后的165个氨基酸组成，理论分子量为19.2 kDa。本产品在还原性SDS-PAGE中呈现出约20和22 kDa的双条带，这反映了其天然的糖基化修饰状态，纯度经SDS-PAGE检测大于95%。

产品特性

• 种属来源：人 (Homo sapiens)
• 表达宿主：人源细胞表达系统，更接近天然蛋白的翻译后修饰
• 蛋白序列：Met1-Arg191，与人源及猕猴序列相同
• 活性：经功能活性检测，能够特异性刺激人脐静脉内皮细胞(HUVEC)增殖，ED₅₀为4-16 ng/mL
• 产品形式：冻干粉，冻干缓冲液为无菌的100 mM Glycine, 10 mM NaCl, pH 7.0溶液

质量控制

本品在生产过程中经过严格的质量控制，确保其高纯度和生物活性。通过SDS-PAGE分析验证其纯度高于95%，并通过细胞增殖活性实验确认其功能可靠性。

使用说明

复溶建议：开盖前请先离心。为获得最佳效果，建议使用产品配套的复溶缓冲液进行复溶，初始浓度不低于100 µg/mL，用户可根据具体实验需求进一步稀释。

保存条件：冻干粉形式的产品应保存于-20℃以下的干燥环境中。复溶后的溶液可在4℃稳定保存2-7天，长期保存请置于-20℃以下，并建议添加载体蛋白（如0.1% HSA或BSA）以增强稳定性，避免反复冻融。

运输条件：常温运输。

背景信息

血管内皮生长因子(VEGF)，亦称为血管通透因子(VPF)，是血管内皮细胞特异性的肝素结合生长因子。它在体内能够有效诱导血管新生过程，在心血管研究、肿瘤生物学及组织工程等领域具有重要的研究价值。

产品规格与价格

注意：本产品仅供研究使用，不适用于人类或临床诊断。使用前请仔细阅读产品说明书及相关文献。

基因ID：7422 | UniProt ID：P15692

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Vascular Endothelial Growth Factor 165 (VEGFA) - Expressed in Human Cells, High Purity

Catalog Number: PRP100240

Product Name: Human VEGF165 protein

Product Overview

Recombinant Human Vascular Endothelial Growth Factor 165 (VEGFA) is a tag-free recombinant protein produced in a human cell expression system. The amino acid sequence of this protein is derived from the human VEGF165 isoform (P15692-4), consisting of 165 amino acids after signal peptide removal, with a theoretical molecular mass of 19.2 kDa. This product displays a double band of approximately 20 and 22 kDa in reducing SDS-PAGE, reflecting its natural glycosylation status, and has a purity of >95% as determined by SDS-PAGE analysis.

Product Features

• Species: Human (Homo sapiens)
• Expression Host: Human cell expression system, providing post-translational modifications closer to the native protein
• Protein Sequence: Met1-Arg191, identical to human and cynomolgus monkey sequences
• Activity: Functionally tested for its ability to specifically stimulate the proliferation of Human Umbilical Vein Endothelial Cells (HUVEC), with an ED₅₀ of 4-16 ng/mL
• Form: Lyophilized powder, lyophilized from a sterile solution containing 100 mM Glycine, 10 mM NaCl, pH 7.0

Quality Control

This product undergoes stringent quality control during manufacturing to ensure high purity and biological activity. Purity is verified to be >95% by SDS-PAGE analysis, and functional reliability is confirmed by cell proliferation activity assays.

Usage Instructions

Reconstitution Recommendation: Centrifuge the vial briefly before opening. For optimal results, it is recommended to reconstitute the protein using the provided reconstitution buffer to an initial concentration of no less than 100 µg/mL. Users can further dilute the product according to specific experimental requirements.

Storage Conditions: The lyophilized product should be stored dry at below -20°C. The reconstituted solution can be stored at 4°C for 2-7 days. For long-term storage, aliquot and store at below -20°C. Adding a carrier protein (e.g., 0.1% HSA or BSA) is recommended to enhance stability. Avoid repeated freeze-thaw cycles.

Shipping Conditions: Shipped at ambient temperature.

Background Information

Vascular Endothelial Growth Factor (VEGF), also known as Vascular Permeability Factor (VPF), is a heparin-binding growth factor specific for vascular endothelial cells. It potently induces angiogenesis in vivo and holds significant research value in cardiovascular research, tumor biology, and tissue engineering.

Specifications and Pricing

Note: This product is for research use only. It is not intended for human or clinical diagnostic use. Please read the product manual and relevant literature carefully before use.

Gene ID: 7422 | UniProt ID: P15692

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.