LIF细胞因子,重组人白血病抑制因子,重组蛋白,E.coli

Human LIF Protein, His‑tag (Animal‑Free) – PRP1099

产品名称： LIF细胞因子（重组人白血病抑制因子）
英文名称： Human LIF Protein, His tag (Animal‑Free)
货号： PRP1099
分类： 细胞因子 – 其它产品 → 细胞因子及蛋白 → 细胞因子

产品概述

本产品为来源于大肠杆菌（E.coli）的重组人白血病抑制因子（LIF），在N‑端带有6×His标签（His‑tag），采用无动物源表达体系。氨基酸序列对应人LIF (Ser24‑Phe220)（UniProt ID: P15018），分子量计算为20.52 kDa，SDS‑PAGE 还原条件下迁移至约18 kDa。

关键参数

参数	内容
种属	人
蛋白长度	20.52 kDa（计算），18 kDa（SDS‑PAGE）
标签	6×His（N‑端）
纯度	> 98 %（SDS‑PAGE检测）
活性测定	TF‑1细胞增殖，ED₅₀ < 0.2 ng/mL
内毒素	≤ 0.1 EU/µg（LAL法）
表达宿主	大肠杆菌（E.coli）
产品形态	0.2 µm过滤冻干粉（含1×PBS，pH 7.4）
保存条件	冻干：‑20 °C ≤ 1 年；复溶后2‑8 °C ≤ 1 周；稀释后‑20 °C／‑80 °C 3‑6 月
运输方式	冰袋（蓝冰）

应用领域

本LIF蛋白适用于细胞培养中的多种研究需求，尤其是：

• 维持和诱导多能干细胞（ESC、iPSC）的自我更新
• 促进造血系细胞（如TF‑1、DA1）增殖
• 调控成骨细胞、胶质细胞及M1型白血病细胞的分化
• 探索LIF在神经保护、免疫调节及肿瘤微环境中的功能

使用指南

1. 使用前请先离心去除可能的微粒。
2. 推荐使用本公司配套的Reconstitution Buffer（浓度≥100 µg/mL）复溶冻干粉。
3. 复溶后可根据实验需求进行适当稀释；建议在含0.1 % BSA、10 % FBS或5 % HSA的缓冲体系中保存，以提高蛋白稳定性。
4. 避免重复冻融循环，以免影响活性。

包装与规格

规格	价格（人民币）	货期
5 µg	588	现货（次日发货）
20 µg	1498	现货（次日发货）
100 µg	3216	请咨询
1 mg	请咨询	请咨询

安全与合规

警告：本品仅限科研使用，不得用于临床诊断或治疗。用户应确保使用过程不侵犯任何专利或许可协议，因违规使用导致的法律责任由使用者自行承担。

技术支持

如有产品性能、配方或实验方案的特殊需求，欢迎随时联系我们的技术服务团队。我们提供快速响应的专业咨询，以帮助您实现实验目标。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human LIF Protein, His‑tag (Animal‑Free) – PRP1099

Product Name: LIF Cytokine (Recombinant Human Leukemia Inhibitory Factor)
English Name: Human LIF Protein, His tag (Animal‑Free)
Catalog No.: PRP1099
Category: Cytokines – Other Products → Cytokines & Proteins → Cytokines

Product Overview

This product is a recombinant human leukemia inhibitory factor (LIF) expressed in E.coli, bearing an N‑terminal 6×His tag, produced using an animal‑free expression system. The amino‑acid sequence corresponds to human LIF (Ser24‑Phe220) (UniProt ID: P15018), with a calculated molecular weight of 20.52 kDa and migration at ~18 kDa under reducing SDS‑PAGE conditions.

Key Parameters

Parameter	Details
Species	Human
Protein Length	20.52 kDa (calculated), 18 kDa (SDS‑PAGE)
Tag	6×His (N‑terminal)
Purity	> 98 % (SDS‑PAGE analysis)
Activity Assay	TF‑1 cell proliferation, ED₅₀ < 0.2 ng/mL
Endotoxin	≤ 0.1 EU/µg (LAL assay)
Expression Host	Escherichia coli
Product Form	0.2 µm filtered lyophilized powder (contains 1× PBS, pH 7.4)
Storage Conditions	Lyophilized: ‑20 °C ≤ 1 year; Reconstituted 2‑8 °C ≤ 1 week; Diluted ‑20 °C/‑80 °C 3‑6 months
Shipping Method	Ice pack (blue ice)

Application Areas

This LIF protein is suitable for a variety of cell‑culture research needs, especially:

• Maintaining and inducing self‑renewal of pluripotent stem cells (ESCs, iPSCs)
• Promoting proliferation of hematopoietic cells (e.g., TF‑1, DA1)
• Modulating differentiation of osteoblasts, glial cells, and M1‑type leukemia cells
• Exploring LIF functions in neuroprotection, immune regulation, and tumor microenvironment

Usage Guidelines

1. Prior to use, centrifuge to remove any particulates.
2. Reconstitute the lyophilized powder with the company‑provided Reconstitution Buffer (concentration ≥100 µg/mL).
3. After reconstitution, dilute as needed for your assay; it is recommended to store the solution in buffers containing 0.1 % BSA, 10 % FBS, or 5 % HSA to enhance protein stability.
4. Avoid repeated freeze‑thaw cycles to preserve activity.

Packaging and Specifications

Specification	Price (CNY)	Lead Time
5 µg	588	In stock (next‑day shipment)
20 µg	1498	In stock (next‑day shipment)
100 µg	3216	Please inquire
1 mg	Please inquire	Please inquire

Safety and Compliance

Warning: This product is for research use only and must not be used for clinical diagnosis or therapy. Users are responsible for ensuring that their use does not infringe any patents or licensing agreements; any legal liability arising from non‑compliant use shall be borne by the user.

Technical Support

If you have special requirements regarding product performance, formulation, or experimental protocols, please contact our technical service team. We provide rapid, professional assistance to help you achieve your experimental goals.

Note: The information above may change due to product optimization or upgrades. For the most current details, please contact YaKoin Company.