β-NGF细胞因子,重组小鼠神经生长因子-β,重组蛋白,E.

产品概述

Mouse β-NGF protein, His tag (Animal-Free)（货号：PRP1143）是一种通过大肠杆菌表达系统重组表达的高纯度小鼠源神经生长因子-β蛋白片段。该产品C末端带有6×His标签，适用于细胞培养等相关研究应用，具备高生物活性与严格的质控标准。

产品详情

• 英文名称：Mouse β-NGF protein, His tag (Animal-Free)
• 货号：PRP1143
• 来源：小鼠 β-NGF（UniProt: P01139），氨基酸序列 Met121-Gly241
• 表达系统：大肠杆菌（E.coli）
• 纯度：＞98%（SDS-PAGE 检测）
• 分子量：14.41 kDa（理论值），SDS-PAGE 迁移范围约为 11–17 kDa
• 标签：C末端 6×His
• 生物活性：ED₅₀ < 1 ng/mL，基于TF-1细胞增殖实验，比活性 > 1×10⁶ IU/mg
• 内毒素水平：＜0.1 EU/μg（LAL法检测）

产品形式与处理建议

本品为冻干形式，由经0.2 µm过滤的20 mM柠檬酸钠与0.2 M NaCl（pH 4.5）缓冲液中制备而成。

• 复溶建议：使用无菌ddH₂O复溶至浓度不低于100 μg/mL，室温静置≥20分钟，避免振荡。
• 保存条件：冻干粉可于-20℃保存一年；复溶后于2–8℃可保存一周。进一步稀释于含载体蛋白（如0.1% BSA）后可于-20℃或-80℃保存3–6个月，建议避免反复冻融。
• 运输条件：冰袋（蓝冰）运输。

背景信息

β-神经生长因子（β-Nerve Growth Factor, β-NGF）是神经营养素家族成员，是一个与其生物活性直接相关的亚单位。它通过结合p75神经营养素受体和Trk受体，参与调控神经细胞的存活与凋亡过程。本品为动物源游离（Animal-Free）表达，适用于多种细胞生物学研究场景。

规格与价格

注意事项

本品仅用于科学研究，不适用于人类或临床诊断。用户须自行确认使用方式符合相关法规或知识产权要求。

更多产品信息或需要其他规格，请联系我们获取详细资料。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Mouse β-NGF protein, His tag (Animal-Free) (Catalog No.: PRP1143) is a recombinant, high‑purity mouse‑derived β‑nerve growth factor protein fragment expressed in an Escherichia coli system. The product carries a C‑terminal 6×His tag and is suitable for cell culture and related research applications, offering high biological activity and stringent quality control standards.

Product Details

• English Name: Mouse β-NGF protein, His tag (Animal-Free)
• Catalog No.: PRP1143
• Source: mouse β-NGF (UniProt: P01139), amino‑acid sequence Met121‑Gly241
• Expression System: Escherichia coli (E. coli)
• Purity: >98% (SDS‑PAGE analysis)
• Molecular Weight: 14.41 kDa (theoretical), SDS‑PAGE migration range approximately 11–17 kDa
• Tag: C‑terminal 6×His
• Biological Activity: ED₅₀ < 1 ng/mL, based on TF‑1 cell proliferation assay, specific activity > 1×10⁶ IU/mg
• Endotoxin Level: <0.1 EU/μg (LAL assay)

Product Formulation and Handling Recommendations

The product is supplied as a lyophilized powder prepared in 20 mM sodium citrate / 0.2 M NaCl buffer (pH 4.5) that has been filtered through a 0.2 µm membrane.

• Reconstitution Recommendation: Reconstitute with sterile ddH₂O to a concentration of at least 100 µg/mL, incubate at room temperature for ≥20 min, and avoid vortexing.
• Storage Conditions: Lyophilized powder can be stored at –20 °C for up to one year; after reconstitution, it is stable at 2–8 °C for one week. When further diluted in a carrier protein solution (e.g., 0.1 % BSA), the product can be stored at –20 °C or –80 °C for 3–6 months; repeated freeze‑thaw cycles should be avoided.
• Shipping Condition: Transport on ice packs (blue ice).

Background Information

β‑Nerve Growth Factor (β‑NGF) is a member of the neurotrophin family and represents the bioactive subunit directly responsible for its physiological effects. It mediates neuronal survival and apoptosis by binding to the p75 neurotrophin receptor and Trk receptors. This product is expressed in an animal‑free system and is suitable for a variety of cell‑biology research applications.

Specifications and Pricing

Precautions

This product is for scientific research use only and is not intended for human or clinical diagnostic applications. Users are responsible for ensuring that their use complies with applicable regulations and intellectual‑property requirements.

For additional product information or to request alternative specifications, please contact us for details.

Note: The above information may change due to product optimisation, upgrades, or other factors; please consult Yakeyin Co. for the most current details.