IL-1α细胞因子,重组人白介素1-α,重组蛋白,E.col

重组人白介素1-α (IL-1α) 蛋白，His标签 (无动物源)

货号：PRP1003

产品类型：细胞因子

产品中心：细胞因子及蛋白

产品分类：核心产品

产品概述

重组人IL-1α蛋白是一种通过大肠杆菌表达系统制备的高纯度、高活性细胞因子。该蛋白采用无动物源生产工艺，并带有His标签，便于后续的纯化与检测。其在细胞培养与免疫学研究中具有重要应用价值，能够有效诱导细胞增殖与炎症因子产生。

关键特性

• 来源：人源IL-1α (Ser113-Ala271)，UniProt编号：P01583
• 表达宿主：大肠杆菌（E.coli）
• 分子量：19.0 kDa（预测值）
• 纯度： > 98%（SDS-PAGE检测）
• 生物活性：ED₅₀ < 10 pg/mL，诱导D10.G4.1细胞增殖；比活性 > 1×10⁸ IU/mg
• 内毒素水平： < 0.1 EU/μg（LAL法检测）
• 产品形式：冻干粉，缓冲体系为无菌PBS（pH 8.0）

应用领域

• 细胞增殖与分化研究
• 免疫应答机制研究
• 炎症模型构建
• 细胞因子网络调控研究

使用说明

复溶建议：推荐使用配套复溶缓冲液，复溶浓度不低于100 µg/mL。可根据实验需求进一步稀释。

保存条件：

• 冻干粉可在-20°C稳定保存1年。
• 复溶后置于2~8°C可保存1周。
• 稀释后建议分装并添加载体蛋白（如0.1% BSA、10% FBS、5% HSA），于-20°C或-80°C可保存3~6个月，避免反复冻融。

注意事项：使用前请离心，避免产品损失。

背景信息

白细胞介素1α（IL-1α）是IL-1细胞因子家族成员，广泛表达于胃肠道上皮、肺、肝、肾、内皮细胞和星形胶质细胞中。其前体形式经蛋白酶切割后形成具有生物活性的成熟形式，通过与IL-1受体结合，诱导IL-6、IL-8、TNF等炎症因子产生，在免疫监视和炎症反应中发挥核心作用。

产品别名

IL-1α, IL 1α, Hematopoietin-1, Lymphocyte-Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte, IL-1, IL 1, IL1

规格与价格

规格	价格（元）	货期
5 μg	588	现货（次日发货）
20 μg	1498	现货（次日发货）
100 μg	3969	请咨询
1 mg	请咨询	请咨询

运输条件：冰袋运输（蓝冰）

警告

本品仅供研究使用，不适用于人类或临床诊断。使用前请查阅相关文献并遵守所有适用的法律法规和知识产权限制。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Interleukin-1α (IL-1α) Protein, His‑tag (Animal‑origin Free)

Product No.: PRP1003

Product Type: Cytokine

Product Category: Cytokines and Proteins

Product Classification: Core Product

Product Overview

The recombinant human IL-1α protein is a high‑purity, high‑activity cytokine produced via an Escherichia coli expression system. The protein is manufactured without any animal‑derived components and carries a His‑tag to facilitate downstream purification and detection. It has important applications in cell culture and immunological research, efficiently inducing cell proliferation and inflammatory cytokine production.

Key Features

• Source: Human IL-1α (Ser113‑Ala271), UniProt ID: P01583
• Expression Host: Escherichia coli (E.coli)
• Molecular Weight: 19.0 kDa (theoretical)
• Purity: > 98% (SDS‑PAGE analysis)
• Biological Activity: ED₅₀ < 10 pg/mL, induces proliferation of D10.G4.1 cells; specific activity > 1 × 10⁸ IU/mg
• Endotoxin Level: < 0.1 EU/µg (LAL assay)
• Product Form: Lyophilized powder, reconstituted in sterile PBS (pH 8.0)

Application Areas

• Cell proliferation and differentiation studies
• Immune response mechanism research
• Inflammation model construction
• Cytokine network regulation studies

Usage Instructions

Reconstitution Recommendation: Use the provided reconstitution buffer; reconstitute to a concentration of ≥100 µg/mL. Further dilutions can be made as required for experiments.

Storage Conditions:

• Lyophilized powder stable at –20 °C for up to 1 year.
• After reconstitution, store at 2–8 °C for up to 1 week.
• Aliquot diluted samples and add a carrier protein (e.g., 0.1% BSA, 10% FBS, 5% HSA); store at –20 °C or –80 °C for 3–6 months, avoiding repeated freeze‑thaw cycles.

Precautions: Centrifuge prior to use to prevent product loss.

Background Information

Interleukin‑1α (IL‑1α) is a member of the IL‑1 cytokine family and is widely expressed in gastrointestinal epithelium, lung, liver, kidney, endothelial cells, and astrocytes. Its precursor is proteolytically cleaved to generate a biologically active mature form that binds the IL‑1 receptor, inducing the production of inflammatory mediators such as IL‑6, IL‑8, and TNF. IL‑1α plays a central role in immune surveillance and inflammatory responses.

Product Aliases

IL-1α, IL 1α, Hematopoietin-1, Lymphocyte-Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte, IL-1, IL 1, IL1

Specifications and Pricing

Specification	Price (CNY)	Lead Time
5 μg	588	In stock (next‑day shipment)
20 μg	1498	In stock (next‑day shipment)
100 μg	3969	Please inquire
1 mg	Please inquire	Please inquire

Shipping Conditions: Transport with ice packs (blue ice)

Warning

This product is for research use only and is not intended for human or clinical diagnostic applications. Please consult the relevant literature before use and comply with all applicable laws, regulations, and intellectual‑property restrictions.

Note: The above information may change due to product optimization or upgrades. For the most current details, please contact Yakeyin Company.