CNTF细胞因子,重组大鼠睫状神经营养因子,重组蛋白,E.c

重组大鼠睫状神经营养因子 (Rat CNTF Protein)

货号：PRP1222
产品类型：重组细胞因子蛋白
来源：大肠杆菌表达系统，无标签，高纯度>97%

产品描述

重组大鼠睫状神经营养因子（Recombinant Rat Ciliary Neurotrophic Factor, CNTF）是通过大肠杆菌表达系统制备的一种无标签蛋白。该产品氨基酸序列来源于大鼠源 CNTF（UniProt ID: P20294，Met 1-Met 200），由200个氨基酸组成，计算分子量为22.9 kDa，在还原性SDS-PAGE中呈约24 kDa的单一条带，显示其良好的纯度与一致性。

生物活性

经TF-1细胞增殖实验验证，该CNTF蛋白具有优异的生物活性，其半数有效浓度（ED₅₀）为0.02748 ng/mL，体现了其在极低浓度下仍可有效促进细胞增殖反应的特性。

纯度与品质

本品经SDS-PAGE检测纯度超过97%，且每1 µg蛋白质中内毒素（EU）含量低于1.0（LAL法测定），符合细胞培养级蛋白应用标准。

制剂与保存

产品为液态制剂，缓冲体系为20 mM Tris, 50 mM NaCl, pH 8.0。建议在-20℃以下干燥环境中保存，避免反复冻融。4℃可短期保存2–7天，长期贮存建议添加载体蛋白（如0.1% HSA或BSA）并分装。

应用建议

本品广泛用于神经系统相关细胞培养研究，包括神经元与少突胶质细胞的存活、分化实验，也可用于探索CNTF在多发性硬化、神经退行性疾病模型中的潜在作用机制。使用者可根据具体实验体系进行梯度稀释优化。

规格与价格

• 5 μg：¥588（现货，次日发货）
• 20 μg：¥1498（现货，次日发货）
• 100 μg：¥3998（现货，次日发货）
• 1 mg：请咨询（请联系获取定制报价）

背景信息

睫状神经营养因子（CNTF）属于神经营养因子家族，在神经系统中发挥关键作用，可促进特定神经元群的存活、生长与神经递质合成。其通过结合CNTF受体复合物（CNTFRα–gp130–LIFR）激活下游信号通路，近年来在神经修复和炎症调节研究中备受关注。

注意：本产品仅限于科研用途，不用于人体或临床诊断。使用前请阅读产品说明书并遵循相关实验室安全规范。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Rat Ciliary Neurotrophic Factor (Rat CNTF Protein)

Catalog Number: PRP1222
Product Type: Recombinant Cytokine Protein
Source: E. coli Expression System, Tag-free, High Purity >97%

Product Description

Recombinant Rat Ciliary Neurotrophic Factor (CNTF) is a tag-free protein produced using an E. coli expression system. The amino acid sequence of this product is derived from rat CNTF (UniProt ID: P20294, Met 1-Met 200), consisting of 200 amino acids with a calculated molecular weight of 22.9 kDa. It migrates as a single band of approximately 24 kDa on reducing SDS-PAGE, demonstrating high purity and consistency.

Biological Activity

Validated by TF-1 cell proliferation assay, this CNTF protein exhibits excellent biological activity, with a half maximal effective concentration (ED₅₀) of 0.02748 ng/mL, indicating its potent ability to promote cellular proliferation responses even at very low concentrations.

Purity & Quality

This product shows >97% purity as determined by SDS-PAGE analysis. The endotoxin level is less than 1.0 EU per 1 µg of protein (determined by LAL method), meeting the standards for cell culture-grade protein applications.

Formulation & Storage

The product is supplied as a liquid formulation in a buffer containing 20 mM Tris, 50 mM NaCl, pH 8.0. It is recommended to store in a dry environment at -20°C or below, avoiding repeated freeze-thaw cycles. Short-term storage at 4°C for 2–7 days is acceptable. For long-term storage, adding a carrier protein (e.g., 0.1% HSA or BSA) and aliquoting is advised.

Application Notes

This product is widely used in neurological cell culture research, including studies on neuronal and oligodendrocyte survival and differentiation. It can also be utilized to explore the potential mechanisms of CNTF in models of multiple sclerosis and neurodegenerative diseases. Users should optimize gradient dilutions according to their specific experimental systems.

Specifications & Pricing

• 5 μg: ¥588 (In stock, ships next day)
• 20 μg: ¥1498 (In stock, ships next day)
• 100 μg: ¥3998 (In stock, ships next day)
• 1 mg: Please inquire (Contact for custom quotation)

Background Information

Ciliary Neurotrophic Factor (CNTF) belongs to the neurotrophic factor family and plays a critical role in the nervous system by promoting the survival, growth, and neurotransmitter synthesis of specific neuronal populations. It activates downstream signaling pathways by binding to the CNTF receptor complex (CNTFRα–gp130–LIFR) and has garnered significant attention in recent research on neural repair and inflammation modulation.

Note: This product is intended for research use only, not for human or clinical diagnostic use. Please read the product manual and follow relevant laboratory safety protocols before use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult ArKayn for specific details.