IFN-γ细胞因子,重组大鼠干扰素-γ,重组蛋白,E.col

重组大鼠干扰素-γ (Rat IFN-γ Protein)

产品概述

本品为高纯度、无标签的重组大鼠干扰素-γ (IFN-γ) 蛋白，通过E.coli表达系统重组表达，经严格纯化工艺处理，SDS-PAGE检测纯度高达95%以上。该产品以冻干粉形式提供，复溶后具有良好的生物活性，适用于各类细胞培养及免疫学研究，是探索天然免疫与获得性免疫机制的重要工具。

产品信息

• 产品名称：重组大鼠干扰素-γ
• 英文名称：Rat IFN-γ protein
• 货号：PRP1214
• 表达宿主：大肠杆菌（E.coli）
• 蛋白长度：134个氨基酸
• 预测分子量：14.8 kDa
• 表观分子量（SDS-PAGE还原条件）：约14.8 kDa
• 标签类型：无标签（Native）
• 种属来源：大鼠（Rattus norvegicus）

质量控制

• 纯度：≥ 95%（SDS-PAGE检测）
• 内毒素水平：＜1.0 EU/μg（LAL法检测）
• 产品形式：冻干粉
• 冻干缓冲液：150mM NaCl，20mM Tris，pH 7.0

使用说明

使用前请短暂离心，避免粉末损失。推荐使用原配复溶缓冲液（Reconstitution Buffer）进行复溶，复溶浓度建议不低于100 µg/mL。可根据实验需求进一步稀释。复溶后可在4℃短期保存（2-7天），长期保存需置于-20℃以下，并建议添加0.1% HSA或BSA作为稳定剂，避免反复冻融。

保存与运输

• 保存条件：冻干粉应保存在-20℃以下干燥环境中
• 运输条件：常温运输

背景信息

干扰素-γ（IFN-γ）属于II型干扰素家族，是一种重要的免疫调节细胞因子，主要由自然杀伤细胞（NK）和活化的T淋巴细胞产生。其在激活巨噬细胞、增强抗原呈递、调节Th1/Th2平衡以及抑制病毒复制和肿瘤细胞增殖等方面发挥关键作用。大鼠IFN-γ基因ID为25712，UniProt登录号为P01581。

产品规格与价格

规格	价格（元）	货期
20 μg	588	现货（次日发货）
100 μg	1498	现货（次日发货）
500 μg	4698	现货（次日发货）
1 mg	请咨询	请咨询

注意事项

本产品仅限于科研使用，不适用于人类或临床诊断。使用前请查阅相关文献，确保符合实验设计和法规要求。对于因使用本产品可能涉及的专利或许可问题，使用者需自行承担责任。

别名：Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Rat Interferon-gamma (Rat IFN-γ Protein)

Product Overview

This product is a high-purity, tag-free recombinant rat interferon-gamma (IFN-γ) protein. It is recombinantly expressed in an E. coli expression system and undergoes stringent purification processes, with a purity exceeding 95% as determined by SDS-PAGE. Provided as a lyophilized powder, the reconstituted product exhibits excellent biological activity. It is suitable for various cell culture applications and immunological research, serving as a vital tool for investigating innate and adaptive immune mechanisms.

Product Information

• Product Name: Recombinant Rat Interferon-gamma
• English Name: Rat IFN-γ protein
• Catalog Number: PRP1214
• Expression Host: Escherichia coli (E. coli)
• Protein Length: 134 amino acids
• Predicted Molecular Weight: 14.8 kDa
• Apparent Molecular Weight (Reducing SDS-PAGE): Approximately 14.8 kDa
• Tag Type: Tag-free (Native)
• Species Source: Rat (Rattus norvegicus)

Quality Control

• Purity: ≥ 95% (analyzed by SDS-PAGE)
• Endotoxin Level: <1.0 EU/μg (determined by LAL assay)
• Form: Lyophilized powder
• Lyophilization Buffer: 150 mM NaCl, 20 mM Tris, pH 7.0

Usage Instructions

Briefly centrifuge the vial before use to prevent powder loss. Reconstitution using the provided Reconstitution Buffer is recommended to a final concentration not less than 100 µg/mL. Further dilution can be performed according to experimental needs. After reconstitution, the product can be stored short-term at 4°C (2-7 days). For long-term storage, aliquot and store at -20°C or below, and it is advisable to add 0.1% HSA or BSA as a stabilizer. Avoid repeated freeze-thaw cycles.

Storage and Shipping

• Storage Conditions: Lyophilized powder should be stored dry at -20°C or below.
• Shipping Conditions: Shipped at ambient temperature.

Background

Interferon-gamma (IFN-γ), a member of the type II interferon family, is a crucial immunomodulatory cytokine primarily produced by natural killer (NK) cells and activated T lymphocytes. It plays key roles in activating macrophages, enhancing antigen presentation, regulating Th1/Th2 balance, and inhibiting viral replication and tumor cell proliferation. The rat IFN-γ gene ID is 25712, and its UniProt accession number is P01581.

Specifications and Pricing

Specification	Price (CNY)	Availability
20 μg	588	In Stock (Ships Next Day)
100 μg	1498	In Stock (Ships Next Day)
500 μg	4698	In Stock (Ships Next Day)
1 mg	Please Inquire	Please Inquire

Precautions

This product is intended for research use only and is not suitable for human or clinical diagnostic use. Please review relevant literature before use to ensure compliance with experimental design and regulatory requirements. The user assumes full responsibility for any patent or licensing issues that may arise from the use of this product.

Aliases: Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakyin Company.