Ifa, Ifa8, 重组小鼠干扰素-α1a, His-标签

重组小鼠干扰素-α1a (IFN-α1a), His标签 (无动物源)

货号: PRP1161 | 高纯度研究级细胞因子蛋白

本品为一种高纯度、重组表达的小鼠干扰素-α1a (Interferon alpha 1a) 蛋白，专为严谨的细胞培养及免疫学研究设计。该蛋白通过无动物源的大肠杆菌表达系统生产，并带有6×His纯化标签，有效降低了外源病原体风险，为您的实验提供更高的可靠性和一致性。

产品详情

• 产品名称: 重组小鼠干扰素-α1a, His-标签（无动物源）
• 英文名称: Mouse IFN alpha 1a Protein, His tag (Animal-Free)
• 种属来源: 小鼠 (Mus musculus)
• 表达宿主: 大肠杆菌 (Escherichia coli)
• 蛋白序列: 覆盖UniProt登录号P01572 (Cys24-Lys189)的氨基酸序列，N端融合6×His标签。
• 分子量: 计算分子量为19.93 kDa。在还原性SDS-PAGE分析中，观察到的分子量范围约为17-25 kDa。

关键特性与质量控制

• 高纯度: 经SDS-PAGE凝胶电泳检测，纯度 > 98% 。
• 高生物活性: 通过保护L929细胞免受脑心肌炎病毒(EMC)感染的生物学活性测定，其半数有效剂量(ED₅₀) < 10 pg/mL。特异性活性经测定 > 4 x 10⁷ IU/mg。
• 低内毒素: 通过LAL（鲎试剂）法测定，内毒素含量 < 0.1 EU/μg。
• 产品形式: 本品为经0.2 µm过滤器除菌并冻干后的白色粉末，溶于1X PBS (pH 7.4)缓冲体系中。

应用建议

本品可用于多种基于小鼠模型的细胞水平研究，特别是在涉及先天免疫应答、病毒学研究、以及Jak/STAT信号通路激活机制的实验中。

使用注意事项:

• 开盖前请先离心，以避免粉末损失。
• 推荐使用本公司提供的复溶缓冲液(Reconstitution Buffer)进行复溶，复溶浓度建议不低于100 µg/mL。用户可根据具体实验需求，使用含载体蛋白（如0.1% BSA, 10% FBS, 5% HSA）的缓冲液进行进一步稀释。
• 应避免反复冻融，建议复溶后分装保存。

保存与运输

• 保存条件: 冻干粉可在-20°C条件下稳定保存1年。复溶后，在2-8°C下可保存1周。在含载体蛋白的缓冲液中稀释后，分装液可在-20°C或-80°C下保存3-6个月。
• 运输条件: 所有规格均通过冰袋（蓝冰）低温运输，以确保产品稳定性。

规格与价格

规格	价格 (人民币)	货期
5 μg	588	现货，次日发货
20 μg	1498	现货，次日发货
100 μg	3998	现货，次日发货
1 mg	请咨询	请咨询

警告: 本产品仅限于科研用途，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定。

背景信息: 干扰素α1a (IFN-α1a) 是一种重要的I型干扰素，由白细胞产生。它通过结合细胞表面的干扰素α/β受体(IFNAR)，激活Jak/STAT信号通路，从而诱导多种干扰素刺激基因(ISGs)的表达，在抗病毒免疫和细胞生长调控中发挥核心作用。

别名: Ifa; Ifa8; IFN; IFN α1a; IFN-α1a

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Mouse Interferon-α1a (IFN-α1a), His Tag (Animal‑Free)

Item No: PRP1161 | High‑Purity Research‑Grade Cytokine Protein

This product is a high‑purity, recombinant mouse interferon‑α1a (Interferon alpha 1a) protein, specifically designed for rigorous cell‑culture and immunology studies. The protein is produced in an animal‑free Escherichia coli expression system and carries a 6×His purification tag, effectively reducing the risk of exogenous pathogens and providing greater reliability and consistency for your experiments.

Product Details

• Product Name: Recombinant Mouse Interferon‑α1a, His‑Tag (Animal‑Free)
• English Name: Mouse IFN alpha 1a Protein, His tag (Animal-Free)
• Species Source: Mouse (Mus musculus)
• Expression Host: Escherichia coli
• Protein Sequence: Amino‑acid sequence covering UniProt accession P01572 (Cys24‑Lys189) with an N‑terminal fusion 6×His tag.
• Molecular Weight: Calculated molecular weight is 19.93 kDa. Under reducing SDS‑PAGE, the observed molecular‑weight range is approximately 17‑25 kDa.

Key Characteristics and Quality Control

• High Purity: SDS‑PAGE analysis shows purity > 98%.
• High Biological Activity: Biological activity assay based on protection of L929 cells from encephalomyocarditis virus (EMC) infection yields a half‑effective dose (ED₅₀) of < 10 pg/mL. Specific activity measured at > 4 × 10⁷ IU/mg.
• Low Endotoxin: Measured by LAL (Limulus amebocyte lysate) assay, endotoxin content < 0.1 EU/µg.
• Product Form: The product is a sterile, lyophilized white powder filtered through a 0.2 µm membrane and reconstituted in 1× PBS (pH 7.4) buffer.

Suggested Applications

The product is suitable for a range of mouse‑model‑based cellular studies, especially those involving innate immune responses, virology, and Jak/STAT signaling pathway activation mechanisms.

Usage Precautions:

• Centrifuge before opening to avoid loss of powder.
• We recommend reconstitution with the company‑provided Reconstitution Buffer at a concentration not lower than 100 µg/mL. Users may further dilute with carrier‑protein‑containing buffers (e.g., 0.1 % BSA, 10 % FBS, 5 % HSA) as required.
• Avoid repeated freeze‑thaw cycles; aliquot after reconstitution for storage.

Storage and Shipping

• Storage Conditions: Lyophilized powder is stable at –20 °C for up to 1 year. After reconstitution, it can be stored at 2‑8 °C for up to 1 week. When diluted in carrier‑protein buffer, aliquots can be kept at –20 °C or –80 °C for 3‑6 months.
• Shipping Conditions: All formats are shipped on ice packs (blue ice) under refrigerated conditions to ensure product stability.

Specifications and Pricing

Specification	Price (CNY)	Lead Time
5 µg	588	In stock, next‑day shipment
20 µg	1498	In stock, next‑day shipment
100 µg	3998	In stock, next‑day shipment
1 mg	Please inquire	Please inquire

Warning: This product is for research use only and is not intended for human or clinical diagnostic applications. Users must ensure that their use does not infringe any patents or licensing agreements.

Background: Interferon‑α1a (IFN‑α1a) is a key type‑I interferon produced by leukocytes. It binds the interferon‑α/β receptor (IFNAR) on the cell surface, activates the Jak/STAT signaling cascade, and induces expression of numerous interferon‑stimulated genes (ISGs), playing a central role in antiviral immunity and regulation of cell growth.

Synonyms: Ifa; Ifa8; IFN; IFN α1a; IFN‑α1a

Note: The above information may change due to product optimization or upgrades; please contact Yakoin Inc. for the most current details.