IL-11细胞因子,重组小鼠白介素-11,重组蛋白,E.co

重组小鼠白介素-11 (IL-11) 蛋白，N端His标签 (无动物源)

产品编号：PRP1165
英文全称：Mouse IL-11 Protein, His tag (Animal-Free)
运输条件：蓝冰运输

产品概述

本品为采用大肠杆菌表达系统重组表达的高纯度、无动物源的小鼠白细胞介素-11 (IL-11) 蛋白。其氨基酸序列来源于小鼠IL-11 (Met 22-Asn199, Uniprot ID: P47873)，并在N端融合了6×His标签，便于纯化与检测。该产品经过严格质控，确保其高生物活性与低内毒素水平，是进行细胞培养、信号通路研究及造血相关功能研究的理想工具。

产品特性

• 种属来源：小鼠 (Mouse)
• 表达宿主：大肠杆菌 (E. coli)
• 蛋白标签：N-His
• 分子量：计算分子量为19.96 kDa。在还原性SDS-PAGE中，其表观分子量范围为17-25 kDa。
• 纯度：经SDS-PAGE分析，纯度 > 98%
• 内毒素：通过LAL法测定，每微克蛋白中内毒素含量 < 0.1 EU。
• 生物活性：通过测定其诱导T11细胞增殖的能力进行验证。该效应的半数有效浓度(ED₅₀) < 0.5 ng/mL。
• 产品形式：经0.2 µm过滤后冻干于1X PBS (pH 8.0)缓冲液中。

使用说明

复溶建议：开盖前请先离心。推荐使用我司提供的专用复溶缓冲液(Reconstitution Buffer)进行复溶，复溶浓度建议不低于100 µg/mL。用户可根据后续实验需求，使用含载体蛋白（如0.1% BSA, 10% FBS, 5% HSA）的缓冲液进行进一步稀释。

保存条件：冻干粉可在-20°C下稳定保存1年。复溶后，在2°C至8°C下可保存最多1周。稀释分装后，在-20°C或-80°C下可保存3-6个月。请避免反复冻融。

背景信息

白细胞介素11 (IL-11) 是gp130细胞因子家族的一员，最初因其在造血过程中，特别是在促进巨核细胞成熟和血小板生成中的关键作用而被广泛认知。其功能通过形成gp130/IL-11/IL-11RA复合物二聚体的高阶结构来介导。此外，它也被称为脂肪生成抑制因子(AGIF)，在多种生理过程中扮演重要角色。

规格与价格

警告：本品仅供研究使用，不适用于人类或临床诊断。用户有责任确保其应用不违反任何专利或许可证规定。

更多信息：如需查阅详细蛋白序列，请访问 UniProt (Q9H293)。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Mouse Interleukin-11 (IL-11) Protein, N-terminal His-tag (Animal-Free)

Catalog Number: PRP1165
Full Name: Mouse IL-11 Protein, His tag (Animal-Free)
Shipping Condition: Shipped on blue ice.

Product Overview

This product is a high-purity, animal-free recombinant mouse Interleukin-11 (IL-11) protein expressed in an E. coli expression system. The amino acid sequence is derived from mouse IL-11 (Met 22-Asn199, Uniprot ID: P47873) and includes an N-terminal 6×His tag for purification and detection. This product undergoes rigorous quality control to ensure high biological activity and low endotoxin levels, making it an ideal tool for cell culture, signaling pathway research, and hematopoietic function studies.

Product Features

• Source: Mouse
• Expression Host: E. coli
• Tag: N-His
• Molecular Weight: Calculated molecular mass is 19.96 kDa. The apparent molecular mass ranges from 17-25 kDa as determined by reducing SDS-PAGE.
• Purity: > 98% as determined by SDS-PAGE analysis.
• Endotoxin: < 0.1 EU per μg protein as determined by the LAL method.
• Biological Activity: Verified by its ability to induce T11 cell proliferation. The half-maximal effective concentration (ED₅₀) for this effect is < 0.5 ng/mL.
• Formulation: Lyophilized from 1X PBS (pH 8.0) after 0.2 μm filtration.

Usage Instructions

Reconstitution Recommendation: Centrifuge the vial before opening. It is recommended to reconstitute using the provided Reconstitution Buffer to a concentration not less than 100 μg/mL. For subsequent experiments, further dilution can be performed using buffers containing carrier proteins (e.g., 0.1% BSA, 10% FBS, 5% HSA).

Storage Conditions: The lyophilized powder is stable for 1 year when stored at -20°C. After reconstitution, it can be stored at 2°C to 8°C for up to 1 week. Aliquot and store diluted aliquots at -20°C or -80°C for 3-6 months. Avoid repeated freeze-thaw cycles.

Background

Interleukin-11 (IL-11) is a member of the gp130 cytokine family, initially recognized for its crucial role in hematopoiesis, particularly in promoting megakaryocyte maturation and platelet production. Its function is mediated through the formation of a higher-order complex involving gp130/IL-11/IL-11RA dimerization. Also known as Adipogenesis Inhibitory Factor (AGIF), it plays important roles in various physiological processes.

Specifications & Pricing

Warning: This product is for research use only. Not intended for human or diagnostic use. The user is responsible for ensuring that its application does not infringe any patents or license agreements.

More Information: For detailed protein sequence information, please visit UniProt (Q9H293).

Note: The above information is subject to change due to product optimization and updates. For specific details, please contact AcroBiosystems Co.