Leptin细胞因子,重组人瘦素蛋白,重组蛋白,E.coli

重组人瘦素蛋白 (Human Leptin Protein)

货号: PRP1038

产品形式: 高纯度冻干粉

规格与价格:

• 100 µg - ¥588 (现货，次日发货)
• 1 mg - ¥1588 (货期请咨询)

产品概述

重组人瘦素蛋白是一种由大肠杆菌表达系统重组表达的无标签蛋白，其氨基酸序列来源于人源Leptin (Val22-Cys167)片段。该产品具有高纯度与良好的稳定性，适用于细胞培养类实验中的瘦素信号通路及相关代谢机制研究。

关键特性

• 高纯度: 经SDS-PAGE检测，纯度 > 98%
• 低内毒素: 每微克蛋白中内毒素含量 < 1.0 EU (LAL法测定)
• 无动物源成分: 采用大肠杆菌表达，避免外源病毒污染风险
• 冻干粉形态: 冻干缓冲液为20 mM Tris，150 mM NaCl，pH 7.4，便于复溶与后续实验操作

技术参数

• 蛋白长度: 146个氨基酸
• 预测分子量: 16 kDa
• 种属来源: 人
• 表达系统: 大肠杆菌
• 复溶建议: 推荐使用配套复溶缓冲液，浓度不低于100 µg/mL，后续可根据实验体系稀释

背景信息

瘦素（Leptin）是一种由脂肪细胞分泌的蛋白激素，其血清浓度与机体脂肪含量呈正相关。它通过结合下丘脑中的瘦素受体，参与调控能量平衡、食欲及代谢行为。在能量匮乏状态下（如饥饿），瘦素水平下降，从而促进摄食行为并降低能量消耗。

基因ID: 3952 | UniProt ID: P41159

保存与运输

• 保存条件: 冻干粉应置于-20℃以下干燥环境；复溶后可在4℃短期保存（2-7天），长期储存需分装存于-20℃以下，建议添加0.1% HSA或BSA作为稳定剂
• 运输条件: 冰袋（蓝冰）运输，保障产品稳定性

使用注意事项

• 使用前请离心，确保冻干粉完全沉降
• 复溶时请使用推荐缓冲液，避免蛋白聚集或降解
• 本产品仅限于科研用途，不适用于临床诊断、治疗或其他人体应用

警告: 使用本品前应查阅相关文献并了解所在国家/地区对生物材料的使用规定。对于因使用本产品可能涉及的专利侵权或违规行为，本公司不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Leptin Protein

Catalog Number: PRP1038

Form: High Purity Lyophilized Powder

Size & Price:

• 100 µg - ¥588 (In Stock, Ships Next Day)
• 1 mg - ¥1588 (Please Inquire for Availability)

Product Overview

Recombinant Human Leptin Protein is a tag-free protein recombinantly expressed in an E. coli expression system, with an amino acid sequence derived from the human Leptin (Val22-Cys167) fragment. This product features high purity and excellent stability, making it suitable for research on leptin signaling pathways and related metabolic mechanisms in cell culture experiments.

Key Features

• High Purity: > 98% as determined by SDS-PAGE analysis
• Low Endotoxin: < 1.0 EU per µg of protein (determined by LAL assay)
• Animal-Free: Expressed in E. coli, avoiding the risk of contamination by exogenous viruses
• Lyophilized Form: Lyophilized from a buffer containing 20 mM Tris, 150 mM NaCl, pH 7.4, facilitating reconstitution and subsequent experimental procedures

Technical Parameters

• Protein Length: 146 amino acids
• Predicted Molecular Weight: 16 kDa
• Species: Human
• Expression System: E. coli
• Reconstitution Recommendation: Reconstitute using the provided buffer to a concentration not less than 100 µg/mL; it can be diluted further according to the experimental system.

Background

Leptin is a protein hormone secreted by adipocytes, and its serum concentration is positively correlated with body fat mass. It binds to leptin receptors in the hypothalamus to regulate energy balance, appetite, and metabolic behavior. Under energy-deficient conditions (e.g., starvation), leptin levels decrease, thereby promoting feeding behavior and reducing energy expenditure.

Gene ID: 3952 | UniProt ID: P41159

Storage & Shipping

• Storage Conditions: Lyophilized powder should be stored in a dry environment at -20°C or below. After reconstitution, it can be stored short-term at 4°C (2-7 days); for long-term storage, aliquot and store at -20°C or below, with the addition of 0.1% HSA or BSA as a stabilizer recommended.
• Shipping Conditions: Shipped with ice packs (blue ice) to ensure product stability.

Important Notes

• Centrifuge the vial prior to use to ensure the lyophilized powder is fully settled.
• Use the recommended buffer for reconstitution to avoid protein aggregation or degradation.
• This product is intended for research use only and is not suitable for clinical diagnosis, therapy, or other human applications.

Warning: Users should consult relevant literature and be aware of the regulations governing the use of biological materials in their country/region before using this product. The company assumes no liability for any potential patent infringement or regulatory violations that may arise from the use of this product.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact YaKeyin Company for specific details.