CXCL3 重组小鼠CXC趋化因子配体3 小鼠重组蛋白 细胞

Mouse CXCL3 Protein, His-tag (Animal-Free) — 炎症微环境研究可靠来源

产品概览

本品为大肠杆菌系统表达的重组小鼠 CXC 趋化因子配体 3，含 N 端 His-tag，适用于体外细胞趋化、黏附及 CXCR2 信号通路机制研究。蛋白覆盖天然 Ala28-Ser100 功能域，预测质量 8.72 kDa，SDS-PAGE 检测纯度＞98%，内毒素水平 LAL 法实测＜0.1 EU/µg。

关键性能

• 生物活性：经 BaF3-CXCR2 细胞趋化实验验证，ED₅₀＜80 ng/mL，可线性响应 20–150 ng/mL 区间。
• 低内毒素：Animal-Free 工艺，每批放行均附 LAL 报告，减少细胞背景激活风险。
• 灵活包装：预充无菌 PBS 冻干，5 µg 起订，最小包装支持 384 孔微趋化实验，避免一次性过量复溶。

推荐实验场景

使用与保存

1. 开盖前瞬时离心，将粉末收集至管底。
2. 按 ≥100 µg/mL 用配套复溶缓冲液重悬，轻柔吹打避免起泡。
3. 实验级稀释液可自行补充 0.1% BSA 或 5% HSA，分装后 −20 °C 或 −80 °C 保存。
4. 避免反复冻融；复溶后 2–8 °C 仅稳定 1 周，建议按需分装。

订购信息

技术档案速览

表达系统：E. coli • 标签：N-His • 分子量：8.72 kDa • 纯度：≥98% (SDS-PAGE) • 内毒素：<0.1 EU/µg (LAL) • 运输：冰袋(蓝冰) • 保存：冻干粉 −20 °C 1 年

本产品仅限科研用途，不可用于临床诊疗、人体或动物治疗。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse CXCL3 Protein, His-tag (Animal-Free) — Reliable source for inflammatory microenvironment research

Product Overview

This product is a recombinant mouse CXC chemokine ligand 3 expressed in an Escherichia coli system, containing an N‑terminal His‑tag, suitable for in vitro cell chemotaxis, adhesion, and CXCR2 signaling pathway mechanism studies. The protein encompasses the native Ala28‑Ser100 functional domain, predicted molecular weight 8.72 kDa, SDS‑PAGE‑determined purity >98%, endotoxin level measured by LAL <0.1 EU/µg.

Key Performance

• Bioactivity: validated by BaF3‑CXCR2 chemotaxis assay, ED₅₀ < 80 ng/mL, with linear response in the 20–150 ng/mL range.
• Low endotoxin: Animal‑Free process; each batch release includes an LAL report, minimizing the risk of background cellular activation.
• Flexible packaging: lyophilized in pre‑filled sterile PBS, minimum order 5 µg, smallest package compatible with 384‑well micro‑chemotaxis assays, avoiding excess reconstitution.

Recommended Experimental Scenarios

Use and Storage

1. Perform a brief centrifugation before opening and collect the powder at the bottom of the tube.
2. Resuspend in the provided reconstitution buffer to a concentration ≥100 µg/mL; mix gently to avoid foaming.
3. Experimental diluents may be supplemented with 0.1 % BSA or 5 % HSA; aliquot and store at −20 °C or −80 °C.
4. Avoid repeated freeze‑thaw cycles; the reconstituted solution is stable for only 1 week at 2–8 °C, so aliquot as needed.

Ordering Information

Technical Data Sheet Overview

Expression system: E. coli • Tag: N‑His • Molecular weight: 8.72 kDa • Purity: ≥98 % (SDS‑PAGE) • Endotoxin: <0.1 EU/µg (LAL) • Shipping: Ice pack (blue ice) • Storage: Lyophilized powder at −20 °C for 1 year

This product is for research use only and is not intended for clinical diagnosis, human or animal therapy.

Note: The above information may change due to product optimization or upgrades; please contact Yakoin Co. for the latest details.