HBGF-6, HST-2, 重组蛋白,细胞因子

重组人成纤维细胞生长因子-6 (FGF-6) 蛋白，His 标签 (无动物源)

产品名称 (中文): 重组人成纤维细胞生长因子-6，His-标签（无动物源）
产品名称 (英文): Human FGF-6 Protein, His tag (Animal-Free)
货号: PRP1026

产品概述

本产品是一种高纯度、高生物活性的重组人源成纤维细胞生长因子-6 (FGF-6) 蛋白。该蛋白通过大肠杆菌表达系统重组表达，携带N-末端His标签，并在生产过程中完全无动物源成分，为细胞培养及相关生物医学研究提供了可靠的工具。FGF-6在细胞增殖、分化、血管生成以及肌肉生成等关键生理过程中扮演着重要角色。

产品特性

• 高纯度: 经SDS-PAGE检测，纯度 > 98%。
• 高比活性: 特异性活性 > 1 × 10⁷ IU/mg。其诱导3T3细胞增殖的半数有效浓度 (ED₅₀) < 0.1 ng/mL。
• 低内毒素: 通过LAL法测定，内毒素含量 < 0.1 EU/µg，适用于对内毒素敏感的细胞实验。
• 无动物源 (Animal-Free): 整个生产工艺过程避免了动物源性成分的引入，降低了外源因子污染的风险。
• 明确的序列与分子量: 蛋白序列对应人FGF-6 (Gly41-Ile208, UniProt ID: P10767)，由168个氨基酸组成，预测分子量为19.66 kDa。

技术参数

属性	详情
种属来源	人 (Human)
表达宿主	大肠杆菌 (E. coli)
标签	His Tag
蛋白长度	168个氨基酸
预测分子量	19.66 kDa
产品形式	冻干粉
冻干缓冲液	无菌 20mM sodium citrate, 0.2 M NaCl, pH 3.5

生物活性验证

产品的生物活性通过其对3T3细胞增殖能力的促进作用进行测定，确保每一批次产品都具有稳定可靠的高活性。

使用说明

• 复溶: 开盖前请先离心。建议使用产品推荐的复溶缓冲液（详见产品说明书）进行复溶，初始浓度不低于100 µg/mL，以获得最佳效果。复溶后可根据实验需求进一步稀释。
• 保存:
  • 冻干粉: 建议在-20°C条件下保存，有效期1年。
  • 复溶后: 在2-8°C下可保存1周。
  • 稀释后: 若在含有载体蛋白（如0.1% BSA）或稳定剂的缓冲液中稀释，分装后于-20°C或-80°C下可保存3-6个月。请避免反复冻融。

应用领域

本品是细胞培养领域的核心试剂，特别适用于：

• 干细胞研究与培养
• 肌肉再生机制研究
• 血管生成（Angiogenesis）相关实验
• 细胞增殖与分化研究

规格与价格（人民币）

规格	价格	货期
5 μg	¥588	现货（次日发货）
20 μg	¥1498	现货（次日发货）
100 μg	¥3969	请咨询
1 mg	请咨询	请咨询

运输条件

全程冰袋（蓝冰）运输，以确保产品稳定性。

注意事项

警告: 本产品仅限于研究用途，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定。对于因使用本产品可能导致的专利侵权或其他违规行为，本公司不承担任何责任。

背景信息

成纤维细胞生长因子-6 (FGF-6/HBGF-6/HST-2) 是FGF家族的重要成员，基因ID为2251。它在肌肉的正常再生过程中是必需的，对调控细胞命运具有重要作用。

如需了解更多详细信息（如蛋白序列），请参考UniProt数据库： P10767 (FGF6_HUMAN)

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Fibroblast Growth Factor-6 (FGF-6) Protein, His Tag (Animal-Free)

Product Name (Chinese): Recombinant Human Fibroblast Growth Factor-6, His Tag (Animal-Free)
Product Name (English): Human FGF-6 Protein, His tag (Animal-Free)
Catalog Number: PRP1026

Product Overview

This product is a high-purity, highly bioactive recombinant human Fibroblast Growth Factor-6 (FGF-6) protein. It is recombinantly expressed in an E. coli system, carries an N-terminal His tag, and is produced entirely free of animal-derived components, providing a reliable tool for cell culture and related biomedical research. FGF-6 plays a crucial role in key physiological processes such as cell proliferation, differentiation, angiogenesis, and myogenesis.

Product Features

• High Purity: Purity > 98% as determined by SDS-PAGE.
• High Specific Activity: Specific activity > 1 × 10⁷ IU/mg. The ED₅₀ for inducing 3T3 cell proliferation is < 0.1 ng/mL.
• Low Endotoxin: Endotoxin level < 0.1 EU/µg as determined by LAL assay, suitable for experiments sensitive to endotoxins.
• Animal-Free: The entire production process avoids the introduction of animal-derived components, reducing the risk of contamination by adventitious agents.
• Defined Sequence & Molecular Weight: The protein sequence corresponds to human FGF-6 (Gly41-Ile208, UniProt ID: P10767), consisting of 168 amino acids with a predicted molecular weight of 19.66 kDa.

Technical Parameters

Attribute	Details
Species	Human
Expression Host	E. coli
Tag	His Tag
Protein Length	168 amino acids
Predicted Molecular Weight	19.66 kDa
Form	Lyophilized powder
Lyophilization Buffer	Sterile 20mM sodium citrate, 0.2 M NaCl, pH 3.5

Bioactivity Validation

The biological activity of the product is determined by its ability to stimulate the proliferation of 3T3 cells, ensuring that each batch possesses stable and reliable high activity.

Usage Instructions

• Reconstitution: Centrifuge the vial before opening. It is recommended to reconstitute the lyophilized powder using the recommended reconstitution buffer (see product manual) to an initial concentration of not less than 100 µg/mL for optimal results. After reconstitution, it can be further diluted according to experimental needs.
• Storage:
  • Lyophilized Powder: Store at -20°C. Stable for 1 year.
  • After Reconstitution: Store at 2-8°C for up to 1 week.
  • After Dilution: When diluted in a buffer containing a carrier protein (e.g., 0.1% BSA) or stabilizer, aliquot and store at -20°C or -80°C for 3-6 months. Avoid repeated freeze-thaw cycles.

Applications

This product is a core reagent in the field of cell culture, particularly suitable for:

• Stem cell research and culture
• Mechanisms of muscle regeneration
• Angiogenesis-related experiments
• Cell proliferation and differentiation studies

Specifications & Pricing (RMB)

Specification	Price	Availability
5 μg	¥588	In stock (ships next day)
20 μg	¥1498	In stock (ships next day)
100 μg	¥3967	Please inquire
1 mg	Please inquire	Please inquire

Shipping Conditions

Shipped with ice packs (blue ice) throughout to ensure product stability.

Precautions

Warning: This product is for research use only and is not intended for human or clinical diagnostic use. The user should ensure that its application does not infringe upon any patents or license regulations. The company assumes no responsibility for patent infringement or other violations that may result from the use of this product.

Background Information

Fibroblast Growth Factor-6 (FGF-6/HBGF-6/HST-2) is an important member of the FGF family, with Gene ID 2251. It is essential for normal muscle regeneration and plays a significant role in regulating cell fate.

For more detailed information (e.g., protein sequence), please refer to the UniProt database: P10767 (FGF6_HUMAN)

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please contact Yakeyin Company.