Long R3 IGF-I, Insulin-like gr

产品概览

产品名称：Long R3 IGF‑I（重组人胰岛素样生长因子 1）
英文名称：Human IGF‑1 Protein
货号：PRP1955
别名：Long R3 IGF‑I, Insulin‑like growth factor I, MGF, Somatomedin‑C, IBP1
适用领域：细胞培养（细胞因子及蛋白）
应用层级：细胞类 → 细胞培养 → 核心产品 → 细胞因子

产品简介

本产品为来源于重组人胰岛素样生长因子 1（IGF‑1，UniProt P05019‑1）的71 氨基酸片段（Gly49‑Ala118），在大肠杆菌体系中表达并经SDS‑PAGE检测确认纯度>99%。IGF‑1 属于胰岛素家族，具备调节细胞增殖、代谢及骨基质合成等多重生物学活性，常用于研究细胞增殖信号、骨代谢及生长因子机制。

关键特性 & 优势

• 高纯度：≥99 %，经SDS‑PAGE验证。
• 低内毒素：采用LAL法检测，<1 EU/µg蛋白。
• 活性评估：在MCF‑7细胞中诱导增殖，ED₅₀ = 76.41 ng/mL。
• 稳定性：冻干粉形式，配合无菌20 mM Tris / 150 mM NaCl（pH 7.4）缓冲液复溶。
• 灵活浓度：推荐复溶浓度≥100 µg/mL，后续可依据实验需求稀释。

技术规格

规格与价格

使用指南

1. 取出冻干粉后，先在4 °C离心去除可能的颗粒。
2. 使用配套的Reconstitution Buffer（20 mM Tris, 150 mM NaCl, pH 7.4）复溶，浓度不低于100 µg/mL。
3. 如需更低工作浓度，请在无菌条件下用适当培养基或缓冲液稀释。
4. 建议在使用前进行活性验证（如MCF‑7细胞增殖实验），确保实验 reproducibility。

保存与稳定性

• 冻干粉：‑20 °C 保存，可维持 1 年。
• 复溶后：2 °C–8 °C 保存 ≤ 1 周；加入 0.1 % HSA 或 BSA 后，可在‑20 °C/‑80 °C 保存 3‑6 个月。
• 避免反复冻融循环，以免影响活性。

运输及包装

产品采用蓝冰（冰袋）冷链包装，确保运输全程维持低温，防止蛋白降解。

法规与安全声明

警告：本产品仅限于科研使用，禁止用于人类临床诊断或治疗。使用本品不应侵犯任何专利或许可证。若因使用本品产生专利侵权或其他违规行为，供应方不承担任何法律责任。

背景信息

IGF‑1（亦称Mechano Growth Factor、Somatomedin‑C）是胰岛素家族成员之一，参与调节青少年生长、骨代谢及葡萄糖转运等关键生理过程。其在体内的表达受生长激素调控，缺陷可导致生长迟缓、感音性聋及智力障碍等表型。IGF‑1在体外实验中表现出对骨细胞（如PyMS）葡萄糖摄取与糖原合成的显著促进作用，活性浓度常低于胰岛素。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Product Name: Long R3 IGF‑I (Recombinant Human Insulin-like Growth Factor 1)
English Name: Human IGF‑1 Protein
Catalog Number: PRP1955
Aliases: Long R3 IGF‑I, Insulin-like growth factor I, MGF, Somatomedin-C, IBP1
Field of Application: Cell Culture (Cytokines & Proteins)
Application Hierarchy: Cell Products → Cell Culture → Core Products → Cytokines

Product Description

This product is a 71-amino acid fragment (Gly49-Ala118) derived from recombinant human Insulin-like Growth Factor 1 (IGF‑1, UniProt P05019-1), expressed in an E. coli system and confirmed by SDS-PAGE to have a purity >99%. IGF-1 belongs to the insulin family and possesses multiple biological activities including regulating cell proliferation, metabolism, and bone matrix synthesis. It is commonly used in research on cell proliferation signaling, bone metabolism, and growth factor mechanisms.

Key Features & Advantages

• High Purity: ≥99%, verified by SDS-PAGE.
• Low Endotoxin: <1 EU/µg protein, tested by LAL assay.
• Activity Assessment: Induces proliferation in MCF-7 cells, ED₅₀ = 76.41 ng/mL.
• Stability: Lyophilized powder format, reconstituted with sterile 20 mM Tris / 150 mM NaCl (pH 7.4) buffer.
• Flexible Concentration: Recommended reconstitution concentration ≥100 µg/mL, can be diluted according to experimental needs.

Technical Specifications

Specifications & Pricing

Usage Instructions

1. After removing the lyophilized powder, centrifuge briefly at 4°C to remove any potential particles.
2. Reconstitute using the supplied Reconstitution Buffer (20 mM Tris, 150 mM NaCl, pH 7.4) to a concentration not less than 100 µg/mL.
3. For lower working concentrations, dilute aseptically with appropriate culture medium or buffer.
4. Activity verification (e.g., MCF-7 cell proliferation assay) is recommended prior to use to ensure experimental reproducibility.

Storage & Stability

• Lyophilized powder: Store at -20 °C, stable for 1 year.
• Reconstituted: Store at 2 °C–8 °C for ≤ 1 week; after adding 0.1% HSA or BSA, store at -20 °C/-80 °C for 3-6 months.
• Avoid repeated freeze-thaw cycles to prevent loss of activity.

Shipping & Packaging

The product is shipped via cold chain packaging with blue ice (ice packs) to maintain low temperature throughout transit and prevent protein degradation.

Regulatory & Safety Statements

Warning: This product is intended for research use only. It is not for use in human clinical diagnosis or therapy. Use of this product should not infringe any patents or licenses. The supplier assumes no legal liability for any patent infringement or other violations resulting from the use of this product.

Background Information

IGF-1 (also known as Mechano Growth Factor, Somatomedin-C) is a member of the insulin family involved in key physiological processes such as adolescent growth, bone metabolism, and glucose transport. Its expression in vivo is regulated by growth hormone, and deficiency can lead to phenotypes such as growth retardation, sensorineural deafness, and intellectual disability. In vitro experiments demonstrate that IGF-1 significantly promotes glucose uptake and glycogen synthesis in bone cells (e.g., PyMS), often exhibiting activity at lower concentrations than insulin.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Yakeyin Company for specific details.