Activin B 重组人激活素B 人源重组蛋白 细胞因子

重组人激活素B蛋白 (Activin B Protein, His Tag, Animal-Free)

产品概述

重组人Activin B蛋白（货号：PRP1209）是一种高纯度、动物源游离的细胞因子蛋白，采用大肠杆菌表达系统生产，带His标签，适用于细胞培养及相关生物医学研究。该产品经严格质控，具备优异的生物活性与稳定性，可满足干细胞分化、炎症研究、骨骼重塑及神经发育等多个前沿领域的应用需求。

产品信息

• 产品名称：重组人激活素B蛋白
• 英文名称：Human Activin B Protein, His tag (Animal-Free)
• 货号：PRP1209
• 来源种属：人源
• 表达宿主：大肠杆菌
• 蛋白长度：115个氨基酸（Gly293-Ala407），预测分子量13.5 kDa（实测约13.75 kDa）
• 纯度：> 98%（SDS-PAGE检测）
• 活性（ED₅₀）：< 0.7 ng/mL（诱导K562细胞血红蛋白表达）
• 比活性：> 1.5 × 10⁶ IU/mg
• 内毒素：< 0.1 EU/μg（LAL法测定）

产品形式与保存

• 形态：冻干粉（冻干缓冲液为无菌PBS，pH 8.0）
• 复溶建议：推荐使用配套复溶缓冲液，浓度不低于100 µg/mL
• 保存条件：冻干粉-20°C可保存1年；复溶后2-8°C可保存1周；稀释后分装-20°C或-80°C可保存3-6个月
• 运输条件：蓝冰运输

生物学背景

Activin B属于TGF-β超家族细胞因子，由INHBB基因编码（UniProt ID: P09529），在干细胞分化、炎症反应、骨重塑及神经发育等多种生理过程中发挥关键调控作用。该蛋白可通过激活Smad信号通路，调控FSH分泌并参与多种细胞应答过程。

应用提示

• 适用于细胞培养实验，如K562细胞血红蛋白诱导实验
• 建议在复溶后避免反复冻融，以保持蛋白活性
• 可根据实验需求在含载体蛋白（如BSA、FBS、HSA）的缓冲液中进一步稀释使用

规格与价格

注意：本品仅供科研使用，不适用于人体或临床诊断。使用前请查阅相关文献并遵守所在机构的生物安全规范。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Activin B Protein (Activin B Protein, His Tag, Animal-Free)

Product Overview

Recombinant Human Activin B Protein (Catalog Number: PRP1209) is a high-purity, animal-free cytokine protein produced using an E. coli expression system, featuring a His tag. It is suitable for cell culture and related biomedical research. This product undergoes stringent quality control, exhibits excellent biological activity and stability, and meets the application requirements for various cutting-edge fields, including stem cell differentiation, inflammation research, bone remodeling, and neural development.

Product Information

• Product Name: Recombinant Human Activin B Protein
• English Name: Human Activin B Protein, His tag (Animal-Free)
• Catalog Number: PRP1209
• Species: Human
• Expression Host: E. coli
• Protein Length: 115 amino acids (Gly293-Ala407), Predicted Molecular Weight: 13.5 kDa (Observed approximately 13.75 kDa)
• Purity: > 98% (by SDS-PAGE)
• Activity (ED₅₀): < 0.7 ng/mL (induction of hemoglobin expression in K562 cells)
• Specific Activity: > 1.5 × 10⁶ IU/mg
• Endotoxin Level: < 0.1 EU/μg (determined by LAL assay)

Form and Storage

• Form: Lyophilized powder (lyophilized from sterile PBS, pH 8.0)
• Reconstitution Recommendation: Recommended to reconstitute using the provided reconstitution buffer to a concentration not less than 100 µg/mL
• Storage Conditions: Lyophilized powder can be stored at -20°C for 1 year; Reconstituted product can be stored at 2-8°C for 1 week; Aliquot diluted product and store at -20°C or -80°C for 3-6 months
• Shipping Conditions: Shipped on blue ice

Biological Background

Activin B belongs to the TGF-β superfamily of cytokines, encoded by the INHBB gene (UniProt ID: P09529). It plays a key regulatory role in various physiological processes, including stem cell differentiation, inflammatory responses, bone remodeling, and neural development. This protein activates the Smad signaling pathway, regulates FSH secretion, and is involved in multiple cellular response processes.

Application Notes

• Suitable for cell culture experiments, such as hemoglobin induction assays in K562 cells
• Avoid repeated freeze-thaw cycles after reconstitution to maintain protein activity
• Can be further diluted in buffers containing carrier proteins (e.g., BSA, FBS, HSA) according to experimental needs

Specifications and Pricing

Note: This product is for research use only, not intended for human use or clinical diagnosis. Please review relevant literature and adhere to your institution's biosafety regulations before use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult AcroBiosystems for specific details.