Activin B 人源重组蛋白 细胞因子

Human Activin B Protein, His tag (Animal-Free)

货号：PRP1209

产品概述

本品为高纯度、高活性人源Activin B重组蛋白，采用无动物源体系表达，携带His标签，适用于多种细胞培养与分化研究应用。作为转化生长因子β（TGF-β）超家族成员，Activin B在干细胞分化、炎症反应、骨骼重塑及神经发育等关键生物学过程中发挥重要作用。

产品特性

• 来源种属：人源（Human）
• 表达宿主：大肠杆菌表达系统（Animal-Free）
• 蛋白序列：人Activin B (Gly293-Ala407) (UniProt: P09529)
• 分子量：13.75 kDa（SDS-PAGE检测）
• 蛋白长度：115个氨基酸
• 纯度：> 98%（SDS-PAGE验证）
• 内毒素：< 0.1 EU/μg（LAL法测定）

生物学活性

经K562细胞血红蛋白表达诱导实验验证，其半数有效浓度（ED₅₀）< 0.7 ng/mL，比活性 > 1.5 × 10⁶ IU/mg。

产品形式与复溶建议

本品为冻干粉形式，缓冲体系为无菌PBS（pH 8.0）。建议使用配套复溶缓冲液（Reconstitution Buffer）复溶至浓度不低于100 µg/mL，后续可根据实验需求进行稀释。

保存与运输

• 保存条件：冻干粉可在-20°C稳定保存1年；复溶后于2-8°C可保存1周；稀释后分装保存于-20°C或-80°C可维持3-6个月稳定性。请避免反复冻融。
• 运输条件：蓝冰低温运输。

应用备注

本品可广泛应用于细胞诱导分化、信号通路研究（如Smad磷酸化检测）、FSH调节研究等相关领域。

规格与价格

注意事项

使用前请离心。本品仅供科研使用，不适用于人体或临床诊断。使用前请查阅相关文献并了解所在地区的专利许可要求。

基因ID：3625
别名：Activin Beta B, INHBB, inhibin beta B chain

更多序列信息请参考：UniProt: P09529

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human Activin B Protein, His tag (Animal-Free)

Product No.: PRP1209

Product Overview

This product is a high‑purity, high‑activity recombinant human Activin B protein expressed in an animal‑free system and carries a His tag. It is suitable for a variety of cell culture and differentiation studies. As a member of the transforming growth factor‑beta (TGF‑β) superfamily, Activin B plays critical roles in stem cell differentiation, inflammatory responses, bone remodeling, and neurodevelopment.

Product Characteristics

• Source species: Human
• Expression host: Escherichia coli expression system (Animal-Free)
• Protein sequence: Human Activin B (Gly293‑Ala407) (UniProt: P09529)
• Molecular weight: 13.75 kDa (SDS‑PAGE confirmed)
• Protein length: 115 amino acids
• Purity: > 98% (SDS‑PAGE verification)
• Endotoxin: < 0.1 EU/µg (LAL assay)

Biological Activity

Validated by K562 cell hemoglobin induction assay; the half‑effective dose (ED₅₀) is < 0.7 ng/mL, and the specific activity is > 1.5 × 10⁶ IU/mg.

Product Form and Reconstitution Recommendation

The product is supplied as a lyophilized powder in sterile PBS (pH 8.0). Reconstitute with the provided Reconstitution Buffer to a concentration of ≥ 100 µg/mL; subsequent dilutions can be made according to experimental requirements.

Storage and Shipping

• Storage conditions: Lyophilized powder is stable at –20 °C for 1 year; after reconstitution, it can be stored at 2–8 °C for 1 week; aliquoted and diluted samples stored at –20 °C or –80 °C remain stable for 3–6 months. Avoid repeated freeze‑thaw cycles.
• Shipping condition: Dry ice (cryogenic) temperature‑controlled shipping.

Application Notes

The product can be widely applied in cellular induction and differentiation, signaling pathway studies (e.g., Smad phosphorylation assays), FSH regulation studies, and related fields.

Specifications and Pricing

Precautions

Centrifuge before use. This product is for research purposes only and is not intended for human or clinical diagnostic use. Prior to use, consult relevant literature and be aware of any regional patent licensing requirements.

Gene ID: 3625
Synonyms: Activin Beta B, INHBB, inhibin beta B chain

For additional sequence information, please refer to: UniProt: P09529

Note: The above information may change due to product optimization or upgrades; for the most current details, please contact Yakoin Co.