NRG1/NRG1-β1 - Human NRG1 Beta

Human NRG1 β1 Protein (NRG1/NRG1‑β1) – PRP1954

产品概述

本品为重组人调蛋白‑1 β1（Neuregulin‑1 β1）蛋白，来源于UniProt Q02297（S20‑K241）片段。采用大肠杆菌表达系统，经过高效层析纯化，满足科研级高纯度需求。

核心应用

• 细胞培养：用于活化EGFR/ErbB受体家族，尤其在乳腺癌细胞系（如MCF‑7）中诱导增殖。
• 神经科学：研究神经发育、突触可塑性及神经递质调控机制。
• 信号通路探索：EGFR、ErbB2/3/4 介导的下游磷酸化及基因表达分析。

关键优势

• 活性验证：ED₅₀ = 0.6730 ng/mL（通过诱导 MCF‑7 细胞增殖测定）。
• 高纯度：SDS‑PAGE 检测显示≥99% 纯度。
• 低内毒素：每 µg 蛋白的 LAL 检测值 < 1.0 EU。
• 易于复溶：采用无菌 20 mM Tris / 150 mM NaCl (pH 7.4) 缓冲液，推荐最终浓度 ≥100 µg/mL。

技术规格

项目	内容
产品编号	PRP1954
基因 ID	142445
蛋白 ID	Q02297
氨基酸长度	220 aa
预测分子量	24.04 kDa
表达宿主	大肠杆菌
纯度	> 99%（SDS‑PAGE）
内毒素水平	<1.0 EU/µg（LAL 法）
产品形态	冻干粉
复溶缓冲液	20 mM Tris, 150 mM NaCl, pH 7.4（无菌）
储存条件	-20 °C（冻干）/ 2‑8 °C（复溶后 ≤1 周）
运输方式	冰袋（蓝冰）

使用指南

• 开盖前请先离心去除可能的微颗粒。
• 使用随包装提供的复溶缓冲液，确保最终浓度不低于 100 µg/mL。
• 根据实验需求，可在含 0.1% HSA 或 BSA 的缓冲体系中进一步稀释，最终储存于 -20 °C 或 -80 °C，避免反复冻融。
• 在细胞培养中，建议在培养基中加入 10‑100 ng/mL（具体浓度依据实验设计）进行处理。

包装与规格

规格	含量	单价 (¥)	货期
规格 1	10 µg	588	现货（次日发货）
规格 2	50 µg	1588	现货（次日发货）
规格 3	500 µg	8998	请咨询
规格 4	1 mg	请咨询	请咨询

储存与稳定性

• 冻干产品：-20 °C，建议保存期限 1 年。
• 复溶后：2‑8 °C，最多保存 1 周。
• 添加载体蛋白或稳定剂后：-20 °C 或 -80 °C，保存 3‑6 个月。
• 请避免多次冻融循环，以维持活性。

运输信息

本产品采用蓝冰包装，确保全程冷链运输，抵达后请在收货后尽快移至-20 °C 冷冻保存。

法规声明与使用限制

仅限科研使用，不适用于任何临床诊断或治疗。使用本产品的任何专利或许可证问题均由用户自行负责，我们不承担相关法律责任。

常用别名检索

ARIA, NRG1, GGF, HGL, HRG, HRGA, NDF, SMDF, Acetylcholine receptor‑inducing activity, Glial growth factor, Heregulin, Neu differentiation factor, Sensory and motor neuron‑derived factor

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human NRG1 β1 Protein (NRG1/NRG1-β1) – PRP1954

Product Overview

This product is a recombinant human Neuregulin-1 β1 protein, derived from the UniProt Q02297 (S20-K241) fragment. It is expressed using an E. coli expression system and purified via high-performance chromatography to meet high-purity research-grade requirements.

Core Applications

• Cell Culture: Used to activate the EGFR/ErbB receptor family, particularly for inducing proliferation in breast cancer cell lines (e.g., MCF-7).
• Neuroscience: Research on neural development, synaptic plasticity, and neurotransmitter regulation mechanisms.
• Signaling Pathway Investigation: Analysis of EGFR, ErbB2/3/4-mediated downstream phosphorylation and gene expression.

Key Advantages

• Activity Verified: ED₅₀ = 0.6730 ng/mL (determined by induction of MCF-7 cell proliferation).
• High Purity: ≥99% purity as determined by SDS-PAGE.
• Low Endotoxin: < 1.0 EU per µg protein by LAL assay.
• Easy Reconstitution: Supplied in sterile 20 mM Tris / 150 mM NaCl (pH 7.4) buffer; recommended final concentration ≥100 µg/mL.

Technical Specifications

Item	Specification
Product Number	PRP1954
Gene ID	142445
Protein ID	Q02297
Amino Acid Length	220 aa
Predicted Molecular Weight	24.04 kDa
Expression Host	E. coli
Purity	> 99% (SDS-PAGE)
Endotoxin Level	<1.0 EU/µg (LAL method)
Form	Lyophilized powder
Reconstitution Buffer	20 mM Tris, 150 mM NaCl, pH 7.4 (sterile)
Storage Conditions	-20 °C (lyophilized) / 2-8 °C (after reconstitution, ≤1 week)
Shipping Condition	Ice pack (blue ice)

Usage Guide

• Centrifuge the vial briefly before opening to remove any potential particulates.
• Use the provided reconstitution buffer to achieve a final concentration not less than 100 µg/mL.
• For experimental needs, further dilution can be performed in a buffer system containing 0.1% HSA or BSA. Store the final aliquots at -20 °C or -80 °C, avoiding repeated freeze-thaw cycles.
• For cell culture applications, a treatment concentration of 10-100 ng/mL (specific concentration depends on experimental design) is recommended.

Packaging & Specifications

Specification	Content	Unit Price (¥)	Availability
Size 1	10 µg	588	In Stock (Ships Next Day)
Size 2	50 µg	1588	In Stock (Ships Next Day)
Size 3	500 µg	8998	Please Inquire
Size 4	1 mg	Please Inquire	Please Inquire

Storage & Stability

• Lyophilized product: -20 °C, recommended shelf life 1 year.
• After reconstitution: 2-8 °C, stable for up to 1 week.
• After adding carrier protein or stabilizer: -20 °C or -80 °C, stable for 3-6 months.
• Avoid multiple freeze-thaw cycles to maintain activity.

Shipping Information

This product is shipped with blue ice to ensure cold chain transport. Upon arrival, please transfer it to -20 °C freezer storage as soon as possible.

Regulatory Statement & Use Limitations

For Research Use Only. Not intended for any clinical diagnosis or therapeutic use. The user is solely responsible for any patent or licensing issues related to the use of this product; we assume no related legal liability.

Common Aliases for Retrieval

ARIA, NRG1, GGF, HGL, HRG, HRGA, NDF, SMDF, Acetylcholine receptor-inducing activity, Glial growth factor, Heregulin, Neu differentiation factor, Sensory and motor neuron-derived factor

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.