CCL2细胞因子,重组小鼠趋化因子配体2,重组蛋白,E.co

Mouse CCL2 Protein, His Tag (Animal-Free)

货号: PRP1148
产品名称: 重组小鼠趋化因子配体2 (CCL2) 蛋白，His标签（无动物源）
英文名称: Mouse CCL2 protein , His tag (Animal-Free)

本品为采用大肠杆菌表达系统重组表达的小鼠源CCL2蛋白，带有N端6×His标签。该蛋白经过严格的质量控制，纯度经SDS-PAGE检测大于98%，具有优异的生物活性，是进行趋化因子相关研究的可靠工具。

产品特性

• 高纯度: SDS-PAGE检测纯度 >98%。
• 高活性: 通过其对转染CCR2A的BaF3细胞的趋化吸引实验测定，ED50 < 8 ng/mL。
• 低内毒素: 采用LAL法检测，每1μg蛋白质中的内毒素（EU）含量小于0.1，有效降低对细胞实验的背景干扰。
• 无动物源: 整个生产过程（包括表达宿主和纯化工艺）均无动物源成分，降低了病毒和朊病毒污染的风险，适用于对安全性要求高的研究。

产品描述

重组小鼠CCL2蛋白（Monocyte Chemotactic Protein-1, MCP-1）是一种重要的CC趋化因子，其氨基酸序列来源于小鼠源CCL2（UniProt ID: P10148）的Gln24-Asn148片段。该蛋白的计算分子量为14.65 kDa，在还原性SDS-PAGE中迁移位置约为17-25 kDa。CCL2主要由单核细胞、巨噬细胞和树突状细胞分泌，在免疫调节和炎症反应中发挥核心作用，包括招募单核细胞、记忆T细胞和树突状细胞，参与辅助T细胞的外渗，以及调节血管生成等过程。

技术参数

种属	小鼠 (Mouse)
表达宿主	大肠杆菌 (E.coli)
标签	N端 6×His
蛋白长度	全长片段 (Gln24-Asn148)
分子量	14.65 kDa (计算值)
产品形式	冻干粉，溶于1X PBS (pH 7.4)，并经过0.2 µm过滤

使用说明

复溶建议: 建议使用无菌ddH2O复溶冻干蛋白，浓度不低于100 μg/mL。复溶时，请在室温下静置孵育至少20分钟，以确保蛋白充分溶解。请勿涡旋振荡，以免影响蛋白活性。

保存条件: 冻干粉可在-20°C下稳定保存1年。复溶后，于2-8°C可保存最多1周。若在含有载体蛋白（如0.1% BSA、10% FBS、5% HSA）的缓冲液中进一步稀释分装，可在-20°C或-80°C下保存3-6个月。请避免反复冻融。

运输条件: 本品采用冰袋（蓝冰）运输。

规格与价格

规格	价格 (人民币)	货期
5 μg	588	现货（次日发货）
20 μg	1498	现货（次日发货）
100 μg	3998	请咨询
1 mg	请咨询	请咨询

注意事项

本品仅供研究使用，不适用于人类或临床诊断。使用前，请务必以3000 rpm离心5分钟，收集全部粉末于管底再进行复溶操作。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse CCL2 Protein, His Tag (Animal-Free)

Catalog Number: PRP1148
Product Name: Recombinant Mouse Chemokine (C-C motif) Ligand 2 (CCL2) Protein, His Tag (Animal-Free)
English Name: Mouse CCL2 protein , His tag (Animal-Free)

This product is a recombinant Mouse CCL2 protein with an N-terminal 6×His tag, expressed in an E. coli expression system. It undergoes rigorous quality control, with a purity of >98% as determined by SDS-PAGE, and exhibits excellent biological activity, making it a reliable tool for chemokine-related research.

Features

• High Purity: Purity >98% as determined by SDS-PAGE.
• High Activity: ED50 < 8 ng/mL, measured by its chemotactic effect on BaF3 cells transfected with CCR2A.
• Low Endotoxin: Endotoxin level is <0.1 EU per 1 μg of protein, as detected by the LAL method, effectively minimizing background interference in cell-based assays.
• Animal-Free: The entire production process, including the expression host and purification procedures, is free of animal-derived components, reducing the risk of viral and prion contamination, making it suitable for research requiring high safety standards.

Description

Recombinant Mouse CCL2 Protein (Monocyte Chemotactic Protein-1, MCP-1) is a key CC chemokine. Its amino acid sequence is derived from the Gln24-Asn148 fragment of Mouse CCL2 (UniProt ID: P10148). The calculated molecular weight of the protein is 14.65 kDa, and it migrates at approximately 17-25 kDa under reducing SDS-PAGE conditions. CCL2 is primarily secreted by monocytes, macrophages, and dendritic cells. It plays a central role in immune regulation and inflammatory responses, including recruiting monocytes, memory T cells, and dendritic cells, participating in the extravasation of helper T cells, and regulating angiogenesis.

Technical Parameters

Species	Mouse
Expression Host	E. coli
Tag	N-terminal 6×His
Protein Length	Full-length fragment (Gln24-Asn148)
Molecular Weight	14.65 kDa (Theoretical)
Formulation	Lyophilized powder, solubilized in 1X PBS (pH 7.4) and filtered through a 0.2 µm membrane

Usage

Reconstitution Recommendation: It is recommended to reconstitute the lyophilized protein using sterile ddH₂O to a concentration not less than 100 μg/mL. After adding the solvent, allow the vial to stand at room temperature for at least 20 minutes to ensure complete dissolution. Do not vortex, as this may affect protein activity.

Storage Conditions: The lyophilized powder is stable for 1 year when stored at -20°C. After reconstitution, it can be stored at 2-8°C for up to 1 week. For long-term storage, aliquot the reconstituted solution in a buffer containing a carrier protein (e.g., 0.1% BSA, 10% FBS, 5% HSA) and store at -20°C or -80°C for 3-6 months. Avoid repeated freeze-thaw cycles.

Shipping Conditions: This product is shipped with ice packs (blue ice).

Specifications & Pricing

Specification	Price (RMB)	Availability
5 μg	588	In Stock (Ships Next Day)
20 μg	1498	In Stock (Ships Next Day)
100 μg	3998	Please Inquire
1 mg	Please Inquire	Please Inquire

Note

This product is for research use only and is not intended for human or diagnostic use. Before reconstitution, centrifuge the vial at 3000 rpm for 5 minutes to collect the entire powder at the bottom of the tube.

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please contact Acogen.