FGF21 - Human FGF-21 protein

Human FGF‑21 Protein (FGF21) – Recombinant Cell Factor

产品概览

本品为 重组人成纤维细胞生长因子 21 (FGF‑21)，源自人源序列 FGF21(Q9NSA1)（His29‑Ser209），在大肠杆菌宿主中表达并经高效纯化。产品定位为细胞因子核心产品，专为细胞培养及相关信号通路研究提供可靠的原料。

核心优势

• 纯度 ≥ 98 %（SDS‑PAGE 检测），分子量与理论值 19.4 kDa 完全匹配。
• 采用 LAL 法 检测，每 1 µg 蛋白质中的内毒素含量 ≤ 1.0 EU，适合对内毒素敏感的细胞系。
• 冻干粉形式，配套无菌重组缓冲液（150 mM NaCl, 20 mM Tris, pH 8.0），保证复溶后浓度 ≥ 100 µg/mL。
• 多规格可选，满足从小规模预实验到大批量生产的需求。

技术规格

属性	内容
产品名称	FGF21 - Human FGF‑21 protein / Human FGF‑21 protein
货号	PRP1030
基因/蛋白 ID	基因ID：26291  蛋白ID：Q9NSA1
别名	Fibroblast Growth Factor 21; FGF‑21; FGF21
表达宿主	大肠杆菌
氨基酸长度	181 (His29‑Ser209)
理论分子量	19.4 kDa
纯度	≥ 98 %（SDS‑PAGE）
活性	Null（用于研究）
内毒素水平	≤ 1.0 EU/µg（LAL法）
产品形态	冻干粉（配套 150 mM NaCl / 20 mM Tris, pH 8.0）
存储条件（冻干）	-20 ℃以下干燥环境
溶解后保存	4 ℃保存 2‑7 天；长期保存 -20 ℃以下，建议加入 0.1 % HSA 或 BSA 防止吸附。
运输方式	冰袋（蓝冰）冷链运输

规格与价格

规格	价格 (人民币)	货期
5 µg	588	现货（次日发货）
50 µg	1498	现货（次日发货）
500 µg	7296	现货（次日发货）
1 mg	请咨询	请咨询

使用说明

• 开盖使用前请先离心除去可能的微粒。
• 建议使用本产品配套的 Reconstitution Buffer 复溶，确保最终浓度 ≥ 100 µg/mL。
• 复溶后如需进一步稀释，请根据实验体系（培养基、缓冲体系等）自行配制合适的稀释液。
• 为避免反复冻融对活性产生潜在影响，建议分装后立即使用或加入 0.1 % HSA/BSA 进行保护。

研究背景

FGF21 属于成纤维细胞生长因子（FGF）家族，具有内分泌特性，缺乏肝素结合域，可在血液中循环。它通过 FGF21 受体结合 β‑klotho 共受体，激活多条代谢调控通路，涉及胚胎发育、细胞增殖、形态发生、组织修复以及代谢疾病模型的研究。该因子在细胞培养体系中常用于探讨代谢调节、胰岛素敏感性以及应激响应等生物学问题。

安全与合规

仅限科研使用。本产品不适用于临床或人类诊断。使用本品所涉及的任何专利、许可证问题，均需用户自行负责。对于可能的专利侵权或法规违规，供应方不承担责任。

参考链接

• UniProt 入口: Q9NSA1 - Human FGF21

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human FGF‑21 Protein (FGF21) – Recombinant Cell Factor

Product Overview

This product is recombinant human Fibroblast Growth Factor 21 (FGF‑21), derived from the human sequence FGF21(Q9NSA1) (His29‑Ser209), expressed in an E. coli host system and highly purified. It is positioned as a core cytokine product, specifically designed to provide reliable material for cell culture and related signaling pathway research.

Key Advantages

• Purity ≥ 98 % (as determined by SDS‑PAGE), with a molecular weight matching the theoretical value of 19.4 kDa.
• Tested by the LAL method, endotoxin levels are ≤ 1.0 EU per 1 µg of protein, making it suitable for endotoxin-sensitive cell lines.
• Lyophilized powder format, supplied with sterile reconstitution buffer (150 mM NaCl, 20 mM Tris, pH 8.0), ensuring a post-reconstitution concentration of ≥ 100 µg/mL.
• Multiple package sizes available to meet needs ranging from small-scale pre-experiments to large-scale production.

Technical Specifications

Attribute	Content
Product Name	FGF21 - Human FGF‑21 protein / Human FGF‑21 protein
Catalog Number	PRP1030
Gene/Protein ID	Gene ID: 26291  Protein ID: Q9NSA1
Aliases	Fibroblast Growth Factor 21; FGF‑21; FGF21
Expression Host	E. coli
Amino Acid Length	181 (His29‑Ser209)
Theoretical Molecular Weight	19.4 kDa
Purity	≥ 98 % (SDS‑PAGE)
Activity	Null (For research use)
Endotoxin Level	≤ 1.0 EU/µg (LAL method)
Form	Lyophilized powder (supplied with 150 mM NaCl / 20 mM Tris, pH 8.0)
Storage Conditions (Lyophilized)	Dry environment below -20 °C
Storage After Reconstitution	Store at 4 °C for 2‑7 days; for long-term storage below -20 °C, addition of 0.1 % HSA or BSA is recommended to prevent adsorption.
Shipping Method	Cold chain shipping with ice packs (blue ice)

Packaging & Pricing

Package Size	Price (CNY)	Lead Time
5 µg	588	In Stock (Ships Next Day)
50 µg	1498	In Stock (Ships Next Day)
500 µg	7296	In Stock (Ships Next Day)
1 mg	Please Inquire	Please Inquire

Usage Instructions

• Briefly centrifuge the vial before opening to remove any potential particulates.
• It is recommended to reconstitute using the supplied Reconstitution Buffer to ensure a final concentration of ≥ 100 µg/mL.
• If further dilution is required after reconstitution, prepare an appropriate diluent according to your experimental system (culture medium, buffer system, etc.).
• To avoid potential impact on activity from repeated freeze-thaw cycles, immediate use after reconstitution or addition of 0.1 % HSA/BSA for protection is recommended.

Research Background

FGF21 belongs to the fibroblast growth factor (FGF) family, exhibits endocrine characteristics, lacks a heparin-binding domain, and circulates in the blood. It binds to the FGF21 receptor complexed with the β-klotho co-receptor, activating multiple metabolic regulatory pathways involved in embryonic development, cell proliferation, morphogenesis, tissue repair, and research on metabolic disease models. This factor is commonly used in cell culture systems to investigate biological questions such as metabolic regulation, insulin sensitivity, and stress responses.

Safety & Compliance

For Research Use Only. This product is not intended for clinical use or human diagnostics. The user is solely responsible for addressing any issues related to patents, licenses, or other legal requirements associated with the use of this product. The supplier assumes no liability for potential patent infringement or regulatory non-compliance.

Reference Links

• UniProt Entry: Q9NSA1 - Human FGF21

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.