G-CSF, Colony Stimulating Fact

产品概述

重组小鼠粒细胞集落刺激因子（G-CSF）是一种高纯度、无标签的重组蛋白，专为细胞培养及相关研究应用设计。该产品采用大肠杆菌表达系统制备，具有高生物活性和优异的批间一致性，适用于对细胞因子功能要求严格的实验场景。

产品特性

• 货号: PRP1308
• 种属来源: 小鼠
• 表达宿主: 大肠杆菌表达系统
• 蛋白长度: 178个氨基酸
• 预测分子量: 18.95 KD
• 纯度: > 99% (SDS-PAGE检测)
• 生物活性: ED₅₀ = 2.038 pg/mL (M-NFS-60细胞增殖试验)
• 内毒素水平: < 1.0 EU/μg (LAL法测定)
• 产品形式: 冻干粉 (冻干缓冲液: 20 mM Tris, 150 mM NaCl, pH 7.4)

质量控制

本品经过严格的质量控制检测，包括SDS-PAGE纯度分析、LAL法内毒素检测以及基于M-NFS-60细胞系的生物活性测定，确保产品符合细胞实验要求。

应用说明

重组小鼠G-CSF在造血干细胞增殖、分化和动员研究中具有重要应用价值。研究表明，该细胞因子通过激活多种信号通路（包括Jak2-STAT3和Akt通路）发挥生物学功能，在心肌梗死模型中也显示出改善心脏功能的潜力。

使用建议

• 复溶方法: 推荐使用配套复溶缓冲液，浓度不低于100 μg/mL
• 实验浓度: 请根据具体实验体系进行梯度稀释优化
• 保存条件: 冻干粉可长期保存于-20℃以下；复溶后可在4℃保存2-7天，长期保存请分装置于-20℃以下
• 稳定性: 为保持长期稳定性，建议添加载体蛋白(0.1% HSA或BSA)

规格与价格

背景信息

粒细胞集落刺激因子(G-CSF)是CSF家族中重要的激素样糖蛋白细胞因子，由免疫细胞和内皮细胞等多种细胞类型产生。通过与G-CSF受体相互作用，激活多种信号通路，在造血干细胞的存活、增殖、分化和动员过程中发挥关键作用。

注意事项

• 使用前请先离心
• 避免反复冻融
• 本产品仅供研究使用，不适用于人类或临床诊断

运输条件

冰袋运输（蓝冰）

相关资源

蛋白序列信息: UniProt P09920

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Recombinant Mouse Granulocyte Colony-Stimulating Factor (G-CSF) is a high-purity, tag-free recombinant protein specifically designed for cell culture and related research applications. This product is produced using an E. coli expression system, offering high biological activity and excellent batch-to-batch consistency, making it suitable for experimental scenarios requiring stringent cytokine functionality.

Product Features

• Catalog Number: PRP1308
• Species Source: Mouse
• Expression Host: E. coli Expression System
• Protein Length: 178 amino acids
• Predicted Molecular Weight: 18.95 KD
• Purity: > 99% (as determined by SDS-PAGE)
• Biological Activity: ED₅₀ = 2.038 pg/mL (M-NFS-60 cell proliferation assay)
• Endotoxin Level: < 1.0 EU/μg (determined by LAL method)
• Product Form: Lyophilized powder (Lyophilized from a solution in 20 mM Tris, 150 mM NaCl, pH 7.4)

Quality Control

This product undergoes rigorous quality control testing, including SDS-PAGE purity analysis, LAL endotoxin testing, and bioactivity assays based on the M-NFS-60 cell line, ensuring it meets the requirements for cellular experiments.

Application Notes

Recombinant Mouse G-CSF holds significant application value in studies involving hematopoietic stem cell proliferation, differentiation, and mobilization. Research indicates that this cytokine exerts its biological functions by activating multiple signaling pathways (including the Jak2-STAT3 and Akt pathways) and shows potential for improving cardiac function in myocardial infarction models.

Usage Recommendations

• Reconstitution Method: Recommended to use the provided reconstitution buffer at a concentration not less than 100 μg/mL
• Experimental Concentration: Please perform gradient dilution optimization based on your specific experimental system
• Storage Conditions: Lyophilized powder can be stored long-term at -20°C or below; after reconstitution, it can be stored at 4°C for 2-7 days; for long-term storage, aliquot and store at -20°C or below
• Stability: To maintain long-term stability, it is recommended to add a carrier protein (0.1% HSA or BSA)

Specifications & Pricing

Background Information

Granulocyte Colony-Stimulating Factor (G-CSF) is an important hormone-like glycoprotein cytokine within the CSF family, produced by various cell types including immune cells and endothelial cells. By interacting with the G-CSF receptor and activating multiple signaling pathways, it plays a key role in the survival, proliferation, differentiation, and mobilization of hematopoietic stem cells.

Precautions

• Centrifuge the product briefly before use
• Avoid repeated freeze-thaw cycles
• This product is intended for research use only and is not suitable for human use or clinical diagnosis

Shipping Conditions

Shipped on ice packs (blue ice)

Related Resources

Protein Sequence Information: UniProt P09920

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Yakeyin Company for specific details.