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文献和实验Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
Reagents Buffered phenol red solution DNA plasmid of interest (e.g., GFP expression plasmid) Store DNA plasmid stocks in endotoxin-free H2 O. DNA purification kit (endotoxin-free) (e.g., Qiagen EndoFree Plasmid Maxi Kit, 12362
in a 1.5 ml microcentrifuge tube, invert to mix, and incubate in an ice-water bath for at least 10 minutes. It is possible to place the sample at -20degC overnight at this stage. 2. Centrifuge at 12,000 rpm in a microcentrifuge (Fisher) for 15
resuspended in 3XSSC, and printed onto a poly-L-lysine coated slide. Materials, Reagents & Solutions 96 well alkaline lysis miniprep kit (Edge BioSystems, Gaithersburg, MD) LB Broth (Biofluids, Rockville, MD) Superbroth (Biofluids, Rockville
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