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文献和实验Recipes for Tissue Culture Selective Drug Stocks
, conical 50 ml tube, weigh an amount of G418 equal to the quantity required to prepare either 20 or 40 ml of 100X solution. Example: You need to prepare 20 ml of 100X G418. The stock G418 powder is 495 mg/mg active ingredient. Perform
AMNIOTIC FLUID CULTURES ON COVERSLIPS
Glacial Acetic Acid: FISHER cat # A38-500. Acetic acid, Glacial ACS (Aldehyde free), 500 ml bottle. L-Glutamine: GIBCO cat# 320-5030. L-Glutamine solution 100X (200 mM), 20 ml bottle, store frozen. Methanol
, without CaCl2 , MgCl2 , MgSO4 , 500ml bottle, Store at 4° C. Glacial Acetic Acid: FISHER SCIENTIFIC cat# A38-500. Acetic acid, Glacial ACS (Aldehyde free), 500ml bottle. L-Glutamine: GIBCO cat# 25030-032. L-Glutamine solution 100X (200mM), 20ml bottle
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