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文献和实验by shaking with hand. 12 mM stachyose (E. coli) or 17% w/v Dextran T-20 (P. aeruginosa) 4 mM sodium NAFD ( add NAOH to NAD sol. to pH 6.0) 1 mM imidazole-NAD (pH imidazole to 6.0 with dry NAD Filter suspension through 8 um Millipore membrane filter
recorder. The rate is estimated from the initial slope. To do this, as quickly as possible, add 10 to 20 µl of the liposome suspension to a cuvette containing 0.6 ml of the sugar solution and turning on the chart recorder. An important control
Purification of Myosin Light Chain Kinase
8 mg 8 ml H2O store frozen -20°F 14.DTT 4.63 g 1 M 30 ml H2) store frozen -20°F 15.TLCK 320 mg 20 mg/ml 16 ml H2O pH 16.TPCK 180 mg 20 mg/ml 9 ml EtOH 17.K-PO4buffer 0.3M KH2PO4 10.21g in 250 ml H2O~0.5 L 0.3M K2HPO4 10.21g in 250 ml H2
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