50084350赛默飞生物安全柜配件Thermo Fishe

Beta-galactosidase Reporter Gene Assay (Liquid Form)

of alpha-factor).  Discard any that fail to change color after substrate addition. 3. Grow at 30 C shaking (250 rpm) until the OD600 nm ~ 0.8 (this is usually done overnight). *** This is the trickiest part of the assay, since it is difficult

Cool growth competent E . coli

2O; add 10 ml 250 mM KCl; pH to 7.0 with NaOH Adjust vol to 1 liter; Autoclave. Just before use, add 5 ml sterile 2M MgCl2 per liter TB per liter 10 mM PIPES Na salt 3.35 g 15 mM CaCl2 (Fisher) 2.2 g 250 mM KCl (Aldrich) 18.64g 55

蛋白组学文章1篇：胰腺癌患者门静脉血清的Maldi-TOF分析 希望斑竹加我第一分啦

血糖浓度高于7.0mmol/L，或根据口服糖耐量试验诊断。 2. 细胞系和细胞培养 MIA PaCa2和PANC-1（来源于人原发性胰腺腺癌）、CAPAN-1（来源于人胰腺转移癌）和正常人纤维母细胞（来源于接受隐静脉切除术者的被切除的隐静脉）在培养基中进行培养，培养基中含低糖（5.5 mM）DMEM、10％FCS、2％谷酰胺和0.1％庆大霉素。细胞（约100,00/每烧瓶）接种于含10ml DMEM的75cm2烧瓶内，培养6天后，取出培养基并以200×g离心10分钟，收集上清液（条件培养液）作质普分析备用