50076162赛默飞生物安全柜配件Thermo Fishe

Cool growth competent E . coli

2O; add 10 ml 250 mM KCl; pH to 7.0 with NaOH Adjust vol to 1 liter; Autoclave. Just before use, add 5 ml sterile 2M MgCl2 per liter TB per liter 10 mM PIPES Na salt 3.35 g 15 mM CaCl2 (Fisher) 2.2 g 250 mM KCl (Aldrich) 18.64g 55

Expression Library Screening (Procaryotic) Using AP-Fusion Proteins

% (w/v) maltose + 10 mM MgSO4 with a single colony. Grow at 37°C, shaking for 4-6 hours (do not grow beyond an OD600 of 1.0). Alternatively, grow overnight at 30°C, shaking at 200 rpm. Phage can adhere to dead cells as well as to live ones

Procedures for In Vitro Production of Bovine Embryos

(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15